干扰素alpha 7蛋白抗体
『别名』IFN alpha 7; IFN alpha J1; IFN alphaJ; IFN-alpha-7; IFN-alpha-J1; IFNA 7; IFNA J; IFNA7; IFNA7_HUMAN; Interferon alpha 7; Interferon alpha family, gene 7; Interferon alpha J; Interferon alpha J1; Interferon alpha-7; Interferon alpha-J; Interferon alpha-J1; LeIF J; OTTHUMP00000021136; OTTMUSP00000012102; RP23-139P14.26. 『浓度』干扰素alpha 7蛋白抗体1mg/1ml
『规格』0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
干扰素alpha 7蛋白抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
干扰素alpha 7蛋白抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
ELB0826c 兔皮肤反应因子/炎性因子(SRF/IF)酶联免疫吸附测定试剂盒 Rabbit SRF/IF (Skin Reactive Factor/Inflammatory Factor) ELISA Kit
ELB1620c 兔胆固醇调节元件结合蛋白(SREBP)酶联免疫吸附测定试剂盒 Rabbit SREBP (Sterol Regulatory Element Binding Proteins) ELISA Kit
ELB0398c 兔非红细胞血影蛋白β4 (SPTβN4)酶联免疫吸附测定试剂盒 Rabbit SPTβN4 (Spectrin Beta, Non Erythrocytic 4) ELISA Kit
ELB0714c 兔α-胞衬蛋白(SPTAN1)酶联免疫吸附测定试剂盒 Rabbit SPTAN1 (Alpha-Fodrin) ELISA Kit
ELB0187c 兔斑型POZ蛋白(SPOP)酶联免疫吸附测定试剂盒 Rabbit SPOP (Speckle Type POZ Protein) ELISA Kit
ELB1032c 兔表面活性物质关联蛋白J(SPJ)酶联免疫吸附测定试剂盒 Rabbit SPJ (Surfactant Associated Protein J) ELISA Kit
ELB1274c 兔肺表面活性物质相关蛋白D(SPD)酶联免疫吸附测定试剂盒 Rabbit SPD (Pulmonary Surfactant Associated Protein D) ELISA Kit
ELB1985c 兔肺表面活性物质相关蛋白C(SPC)酶联免疫吸附测定试剂盒 Rabbit SPC (Pulmonary Surfactant Associated Protein C) ELISA Kit
ELB0857c 兔肺表面活性物质相关蛋白B(SPB)酶联免疫吸附测定试剂盒 Rabbit SPB (Pulmonary Surfactant Associated Protein B) ELISA Kit
ELB1660c 兔SPARC样蛋白1(SPARCL1)酶联免疫吸附测定试剂盒 Rabbit SPARCL1 (SPARC Like Protein 1) ELISA Kit
ELB0947c 兔富含半胱氨酸型酸性蛋白(SPARC)酶联免疫吸附测定试剂盒 Rabbit SPARC (Secreted Protein Acidic And Rich In Cysteine) ELISA Kit
ELB0507c 兔肺表面活性物质相关蛋白A(SPA)酶联免疫吸附测定试剂盒 Rabbit SPA (Pulmonary Surfactant Associated Protein A) ELISA Kit
ELB0743c 兔精子蛋白17(Sp17)酶联免疫吸附测定试剂盒 Rabbit Sp17 (Sperm Protein 17) ELISA Kit
ELB0378c 兔Sp100核抗原(Sp100)酶联免疫吸附测定试剂盒 Rabbit Sp100 (Sp100 Nuclear Antigen) ELISA Kit
ELB0445c 兔性别决定区Y框蛋白9(SOX9)酶联免疫吸附测定试剂盒 Rabbit SOX9 (Sex Determining Region Y Box Protein 9) ELISA Kit
ELB1469c 兔性别决定区Y框蛋白3(SOX3)酶联免疫吸附测定试剂盒 Rabbit SOX3 (Sex Determining Region Y Box Protein 3) ELISA Kit
ELB0289c 兔性别决定区Y框蛋白2(SOX2)酶联免疫吸附测定试剂盒 Rabbit SOX2 (Sex Determining Region Y Box Protein 2) ELISA Kit
ELB1417c 兔性别决定区Y框蛋白18(SOX18)酶联免疫吸附测定试剂盒 Rabbit SOX18 (Sex Determining Region Y Box Protein 18) ELISA Kit
ELB0351c 兔性别决定区Y框蛋白1(SOX1)酶联免疫吸附测定试剂盒 Rabbit SOX1 (Sex Determining Region Y Box Protein 1) ELISA Kit
ELB2140c 兔胞外超氧化物歧化酶(SOD3)酶联免疫吸附测定试剂盒 Rabbit SOD3 (Superoxide Dismutase 3, Extracellular) ELISA Kit
ELB1507c 兔线粒体超氧化物歧化酶2(SOD2)酶联免疫吸附测定试剂盒 Rabbit SOD2 (Superoxide Dismutase 2, Mitochondrial) ELISA Kit
ELB1695c 兔超氧化物歧化酶1(SOD1)酶联免疫吸附测定试剂盒 Rabbit SOD1 (Superoxide Dismutase 1, Soluble) ELISA Kit
ELB0744c 兔细胞因子信号转导抑制因子3(SOCS3)酶联免疫吸附测定试剂盒 Rabbit SOCS3 (Suppressors of Cytokine Signaling 3) ELISA Kit
ELB0610c 兔小核糖核蛋白多肽D1(SNRPD1)酶联免疫吸附测定试剂盒 Rabbit SNRPD1 (Small Nuclear Ribonucleoprotein Polypeptide D1) ELISA Kit
ELB2113c 兔小核糖核蛋白多肽C(SNRPC)酶联免疫吸附测定试剂盒 Rabbit SNRPC (Small Nuclear Ribonucleoprotein Polypeptide C) ELISA Kit
ELB0908c 兔抗小核糖核蛋白/Sm抗体(snRNP/Sm)酶联免疫吸附测定试剂盒 Rabbit snRNP/Sm (Small Nuclear Ribonucleoprotein) ELISA Kit
ELB0449c 兔突触核蛋白γ(SNCγ)酶联免疫吸附测定试剂盒 Rabbit SNCγ (Synuclein Gamma) ELISA Kit
ELB0882c 兔突触核蛋白α(SNCα)酶联免疫吸附测定试剂盒 Rabbit SNCα (Synuclein Alpha) ELISA Kit
ELB0504c 兔末端运动神经元生存蛋白1(SMN1)酶联免疫吸附测定试剂盒 Rabbit SMN1(Survival of Motor Neuron 1, Telomeric) ELISA Kit
ELB1440c 兔平滑肌肌球蛋白(SMM)酶联免疫吸附测定试剂盒 Rabbit SMM (Smooth Muscle Myosin) ELISA Kit
ELB2084c 兔特异性巨噬细胞武装因子(SMAF)酶联免疫吸附测定试剂盒 Rabbit SMAF (Specific Macrophage Arming Factor) ELISA Kit
ELB1714c 兔Smad同源物7 (Smad7/MADH7)酶联免疫吸附测定试剂盒 Rabbit Smad7/MADH7 (Mothers Against Decapentaplegic Homolog 7) ELISA Kit
ELB1033c 兔Smad同源物4 (Smad4/MADH4)酶联免疫吸附测定试剂盒 Rabbit Smad4/MADH4 (Mothers Against Decapentaplegic Homolog 4 )ELISA Kit
ELB0308c 兔Smad同源物3 (Smad3/MADH3)酶联免疫吸附测定试剂盒 Rabbit Smad3/MADH3 (Mothers Against Decapentaplegic Homolog 3) ELISA Kit
ELB0675c 兔Smad同源物1 (Smad1/MADH1)酶联免疫吸附测定试剂盒 Rabbit Smad1/MADH1 (Mothers Against Decapentaplegic Homolog 1) ELISA Kit
ELB1246c 兔鞘磷脂(SM/SPH)酶联免疫吸附测定试剂盒 Rabbit SM/SPH (Sphingomyelin) ELISA Kit
ELB1794c 兔分泌性白细胞蛋白酶抑制因子(SLPI)酶联免疫吸附测定试剂盒 Rabbit SLPI (Secretory Leukocyte Protease Inhibitor) ELISA Kit
ELB0082c 兔Slit同源物2(Slit2)酶联免疫吸附测定试剂盒 Rabbit Slit2 (Slit Homolog 2) ELISA Kit
ELB0519c 兔二级淋巴组织趋化因子(SLC/CCL21)酶联免疫吸附测定试剂盒 Rabbit SLC/CCL21 (Secondary Lymphoid Tissue Chemokine) ELISA Kit
ELB2155c 兔唾液酸结合免疫球蛋白样凝集素8(SIGLEC8)酶联免疫吸附测定试剂盒 Rabbit SIGLEC8 (Sialic Acid Binding Ig Like Lectin 8) ELISA Kit
ELB0870c 兔唾液酸结合免疫球蛋白样凝集素3(SIGLEC3)酶联免疫吸附测定试剂盒 Rabbit SIGLEC3 (Sialic Acid Binding Ig Like Lectin 3) ELISA Kit
ELB0359c 兔唾液酸结合免疫球蛋白样凝集素2(SIGLEC2)酶联免疫吸附测定试剂盒 Rabbit SIGLEC2 (Sialic Acid Binding Ig Like Lectin 2) ELISA Kit
ELB0467c 兔唾液酸结合免疫球蛋白样凝集素12(SIGLEC12)酶联免疫吸附测定试剂盒 Rabbit SIGLEC12 (Sialic Acid Binding Ig Like Lectin 12) ELISA Kit
ELB1342c 兔唾液酸结合免疫球蛋白样凝集素10(SIGLEC10)酶联免疫吸附测定试剂盒 Rabbit SIGLEC10 (Sialic Acid Binding Ig Like Lectin 10) ELISA Kit
ELB0462c 兔唾液酸结合免疫球蛋白样凝集素1(SIGLEC1)酶联免疫吸附测定试剂盒 Rabbit SIGLEC1 (Sialic Acid Binding Ig Like Lectin 1) ELISA Kit
ELB2214c 兔分泌型免疫球蛋白A(sIgA)酶联免疫吸附测定试剂盒 Rabbit sIgA (Secretory Immunoglobulin A) ELISA Kit
ELB0889c 兔可溶性细胞间粘附分子1(sICAM-1)酶联免疫吸附测定试剂盒 Rabbit sICAM-1 (Soluble Intercellular Adhesion Molecule-1) ELISA Kit
ELB2041c 兔唾液酸乙酰酯酶(SIAE)酶联免疫吸附测定试剂盒 Rabbit SIAE (Sialic Acid Acetylesterase) ELISA Kit
ELB0342c 兔音猬因子(SHH )酶联免疫吸附测定试剂盒 Rabbit SHH (Hedgehog Homolog, Sonic) ELISA Kit
ELB1931c 兔SH2携带蛋白(SHC/SLP-76)酶联免疫吸附测定试剂盒 Rabbit SHC/SLP-76 (SH2-Containing Protein) ELISA Kit
ELB1101c 兔性激素结合球蛋白(SHBG)酶联免疫吸附测定试剂盒 Rabbit SHBG (Sex Hormone Binding Globulin) ELISA Kit
ELB1611c 兔可溶性糖蛋白130(sgp130)酶联免疫吸附测定试剂盒 Rabbit sgp130 (soluble Glucoprotein 130) ELISA Kit
ELB0665c 兔血清/糖皮质激素调节激酶2(SGK2)酶联免疫吸附测定试剂盒 Rabbit SGK2 (Serum/Glucocorticoid Regulated Kinase 2) ELISA Kit
ELB0120c 兔分泌型卷曲相关蛋白2 (SFRP2)酶联免疫吸附测定试剂盒 Rabbit SFRP2 (Secreted Frizzled Related Protein 2) ELISA Kit
ELB0463c 兔可溶性血纤蛋白单体复合物(sFMC)酶联免疫吸附测定试剂盒 Rabbit sFMC (Soluble Fibrin Monomer Complex) ELISA Kit
ELB1995c 兔固醇类生成因子1(SF1)酶联免疫吸附测定试剂盒 Rabbit SF1 (Steroidogenic Factor 1) ELISA Kit
ELB0108c 兔血清素转运体(SERT)酶联免疫吸附测定试剂盒 Rabbit SERT (Serotonin Transporter ) ELISA Kit
ELB1724c 干扰素alpha 7蛋白抗体兔血浆硒蛋白P(SEPP1)酶联免疫吸附测定试剂盒 Rabbit SEPP1 (Selenoprotein P) ELISA Kit
ELB0683c 兔可溶性血管内皮细胞蛋白C受体(sEPCR)酶联免疫吸附测定试剂盒 Rabbit SEPCR/PROCR (Soluble Endothelial Protein C Receptor) ELISA Kit
ELB1191c 兔硒蛋白1(SEP1)酶联免疫吸附测定试剂盒 Rabbit SEP1 (Selenoprotein 1) ELISA Kit
ELB1783c 兔信号素7A(SEMA7A)酶联免疫吸附测定试剂盒 Rabbit SEMA7A (Semaphorin 7A) ELISA Kit