B091 γ-谷氨酰半胱氨酸合成酶(γ-GCS)测定试剂盒(速率法) Gamma-glutamylcysteine synthetase 20管 盒
谷氨酰半胱氨酸合成酶(γ-glutamylcysteine synthetase,γ-GCS),是谷胱甘肽(GSH)合成过程中的限速酶。细胞内γ-GCS活性升高使GSH合成加速,从而提高细胞内外GSH的浓度。GSH是机体一种重要的抗氧化剂,在保持气道上皮细胞完整性、抵御肺部损伤与炎症和抗氧化损伤等方面起到重要的作用, 肺脏具有较高浓度的γ-GCS酶活及GSH浓度 (6. 1~17. 5nmol /mg 肺组织) ,呼吸道上皮内衬液中GSH 的浓度(200~400μmol/ l) 是血浆中的100 倍。所以GSH在呼吸道炎症及过敏反应中起了非常重要的作用,并且对心血管疾病及肿瘤的治疗、予后有很大研究价值。
γ-谷氨酰半胱氨酸合成酶(γ-GCS)测定试剂盒(速率法)产品优点如下:
1、快速简便:全程约2小时,可测50例左右样本。
2、稳定性好:试剂盒2~8℃存放6个月有效。
3、再现性好:变异系数CV=1.7%。
4、回收试验: X =103%。
5、受外界影响因素小:干扰因素少,重复性强。
6、测试面广:可测动物血清(浆)、组织等。
所需仪器及自备试剂:
1、紫外分光光度计(比色测定波长为340nm)
2、恒温水浴箱或气浴箱 (孵育温度为37℃)
3、漩涡混匀器
4、微量移液器
批间CV 批内CV 回收率 最底检出线 检测范围
7.85 4.1 103 0.02 U/ml 0.02-10U/ml
本试剂盒2~8℃密封保存,有效期为 6个月。
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γ-谷氨酰半胱氨酸合成酶(γ-GCS)测定试剂盒(速率法)Z(06)-0409 β-Catenin (Phospho-Ser37) 抗体 β-Catenin (Phospho-Ser37) Antibody 50ug或100ug 支
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γ-谷氨酰半胱氨酸合成酶(γ-GCS)测定试剂盒(速率法)Z(06)-0491 TrkB (Phospho-Tyr515) 抗体 TrkB (Phospho-Tyr515) Antibody 50ug或100ug 支
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