γ-谷氨酰半胱氨酸合成酶(γ-GCS)测定试剂盒(速率法)

B091 γ-谷氨酰半胱氨酸合成酶(γ-GCS)测定试剂盒(速率法) Gamma-glutamylcysteine synthetase 20管 盒

谷氨酰半胱氨酸合成酶(γ-glutamylcysteine synthetase，γ-GCS)，是谷胱甘肽(GSH)合成过程中的限速酶。细胞内γ-GCS活性升高使GSH合成加速，从而提高细胞内外GSH的浓度。GSH是机体一种重要的抗氧化剂，在保持气道上皮细胞完整性、抵御肺部损伤与炎症和抗氧化损伤等方面起到重要的作用， 肺脏具有较高浓度的γ-GCS酶活及GSH浓度 (6. 1～17. 5nmol /mg 肺组织) ，呼吸道上皮内衬液中GSH 的浓度(200～400μmol/ l) 是血浆中的100 倍。所以GSH在呼吸道炎症及过敏反应中起了非常重要的作用，并且对心血管疾病及肿瘤的治疗、予后有很大研究价值。   

γ-谷氨酰半胱氨酸合成酶(γ-GCS)测定试剂盒(速率法)产品优点如下：

1、快速简便：全程约2小时，可测50例左右样本。

2、稳定性好：试剂盒2～8℃存放6个月有效。

3、再现性好：变异系数CV=1.7%。

4、回收试验： X =103%。

5、受外界影响因素小：干扰因素少，重复性强。

6、测试面广：可测动物血清（浆）、组织等。

所需仪器及自备试剂：

1、紫外分光光度计（比色测定波长为340nm）

2、恒温水浴箱或气浴箱 （孵育温度为37℃）

3、漩涡混匀器

4、微量移液器

批间CV   批内CV   回收率   最底检出线       检测范围

7.85     4.1      103      0.02 U/ml        0.02-10U/ml

本试剂盒2～8℃密封保存，有效期为 6个月。

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γ-谷氨酰半胱氨酸合成酶(γ-GCS)测定试剂盒(速率法)Z(06)-0409 β-Catenin (Phospho-Ser37) 抗体 β-Catenin (Phospho-Ser37) Antibody 50ug或100ug 支

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γ-谷氨酰半胱氨酸合成酶(γ-GCS)测定试剂盒(速率法)Z(06)-0491 TrkB (Phospho-Tyr515) 抗体 TrkB (Phospho-Tyr515) Antibody 50ug或100ug 支

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