B063-1总巯基(-SH)测定试剂盒Total mercapto (-SH) measurement kit100管/48样(分光光度法)、48T(微板法)盒
活性巯基(SH)主要存在于半胱氨酸,是蛋白质结构和生物体内某些氧化还原反应重要基团。在蛋白质结构中2个巯基脱氢而形成的双硫键,使相邻多肽得以连接.对于维持蛋白质完整结构十分重要。所有动物组织都含有不同量的活性巯基,机体内起重要作用的谷胱甘肽,其生理作用主要是靠所含半胱氨酸中活性巯基起还原作用。巯基还是很多酶活性基团,一些重金属盐如Hg2+等与酶巯基结合,影响酶活性。巯基化合物与机体许多机能活动,药物及毒物的作用以及某些疾病的发生发展有着密切关系。测定组织和血液中巯基含量已日益为人们所重视,以期探索对某些疾病的发生机理、治疗、预防以及预后判断等方面的研究意义。
产品优点如下:
1、快速简便:全程约30分钟,可测90例左右样本。
2、稳定性好:试剂盒2~8℃存放6个月有效。
3、总巯基(-SH)测定试剂盒再现性好:变异系数CV=1.9%
4、回收试验: X =100.3%。
所需仪器及自备试剂:
1、酶标仪(比色测定波长为405nm)
2、恒温水浴箱或气浴箱 (孵育温度为37℃)
3、漩涡混匀器
4、微量移液器
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