B059-1 碱性磷酸酶(ALP)测定试剂盒(可见光比色法) Alkaline phosphatase assay kit 50管/48样 盒
碱性磷酸酶分解磷酸苯二钠,产生游离酚和磷酸,酚在碱性溶液中与4-氨基安替吡啉作用生成红色醌衍生物,根据红色深浅可以测定酶活力的高低。
产品优点如下:
1、快速简便:全程约40分钟,可测80例左右样本;
2、取样量微:常规操作取样量50ul, 酶标仪操作仅需5ull即可测样本中的ALP活力;
3、稳定性好:试剂盒2~8℃存放6个月有效;
4、再现性好:变异系数CV=1.2%,20倍稀释线性仍然良好;
5、回收试验: X =99%;
6、受外界影响因素小:干扰因素少,重复性强;
7、碱性磷酸酶(ALP)测定试剂盒(可见光比色法)测试面广:可测动物血清(浆)、组织、各种体液、灌流液、各种培养细胞以及细胞培养上清液等。
所需仪器及自备试剂:
1、分光光度计/酶标仪/半自动生化分析仪(比色测定波长为520nm)
2、恒温水浴箱或气浴箱 (孵育温度为37℃)
3、漩涡混匀器
4、微量移液器
批间CV 批内CV 回收率 最底检出线 检测范围
3.9 1.2 99 0.05 0.05-50.0
本试剂盒保存期:6个月。贮存温度:2~8℃
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