B041 5'-核苷酸酶(5'-NT)测定试剂盒 5’-nucleotidase assay kit 100管/48样 盒
5'-NT广泛分布于肝脏、胆道系统及各种器官和组织中。血清中此酶活力增高(2~6)主要见于胆道系统疾病,如阻塞性黄疸,原发性及继发性肝癌等,且通常与ALP的活力变化相平行。其增高机理是因高浓度的胆盐促使5'-NT从细胞膜上溶解出来,或膜通透性改变所致,在骨骼系统的疾患如肿瘤转移、畸形性骨炎、甲状旁腺机能亢进、佝偻病等,ALP活力通常增高,而5'-NT仍为正常或为临界值。所以对ALP活力增高患者,测定此酶有助于判断ALP活力增高是胆道系统疾病还是骨骼系统疾病引起。此外,妊娠妇女及儿童期5'-NT不增高,但ALP均升高。
产品优点如下:
1、5'-核苷酸酶(5'-NT)测定试剂盒操作简便:单试剂操作,全程约20分钟,可测50例左右样本。
2、再现性好:批内CV=1.3%,批间CV=4.11%。
3、回收试验: X =103%。
4、受外界影响因素小:干扰因素少,重复性强。
所需仪器及自备试剂:
1、分光光度计/酶标仪/半自动生化分析仪(比色测定波长为530nm)
2、恒温水浴箱或气浴箱 (孵育温度为37℃)
3、漩涡混匀器
4、微量移液器
操作流程:
1、按步骤加入样本
2、混匀,37℃水浴30分钟
3、680nm处1cm
4、680nm处,1cm光径
批间CV 批内CV 回收率 最底检出线 检测范围
5.63 2.8 98 0.1U/ml 0.1-50U/ml
本试剂盒保存期:6个月。贮存温度:2~8℃。
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