B040 总铁结合力(TIBC)测定试剂盒(比色法) total iron binding capacity assay kit 50管/48样 盒
血清内加入过量的铁,使血清中运铁蛋白全部与铁结合。再加入铁的吸附剂将多余的铁吸附掉。然后用测血清铁的方法测定铁的含量,此量称为总铁结合力(TIBC),由TIBC减去血清铁值,则称为未饱和铁结合力(UIBC)。
产品优点如下:
1、操作简便:单试剂操作,全程约20分钟,可测50例左右样本。
2、再现性好:批内CV=1.3%,批间CV=4.11%。
3、回收试验: X =102%。
4、受外界影响因素小:干扰因素少,重复性强。
所需仪器及自备试剂:
1、总铁结合力(TIBC)测定试剂盒(比色法)分光光度计/酶标仪/半自动生化分析仪(比色测定波长为530nm)
2、恒温水浴箱或气浴箱 (孵育温度为37℃)
3、漩涡混匀器
4、微量移液器
操作流程:
1、按步骤加入样本
2、混匀,室温放置5min
3、混匀,520nm波长,0.5cm光径比色
批间CV 批内CV 回收率 最底检出线 检测范围
6.32 3.8 102 0.05mg/L 0.05-30mg/L
本试剂盒保存期:6个月。贮存温度:2~8℃。特别提醒R1、R2需在-20℃以下冷冻保存。
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