B037 糖化血清蛋白(GSP)测定试剂盒(果糖胺法)(带标准) Glycosylated serum protein assay kit 25管/24样、50管/48样 盒
血清蛋白半衰期较短,本试剂有效的反映机体过去1-2周内平均血糖水平.本试剂不受临时血糖浓度波动的影响,故为糖尿病的研究和较长时间血糖控制水平 的研究提供了一个很好的指标。血清葡萄糖能与白蛋白及其它血清蛋白分子N末端的氨基发生非酶促糖化反应,形成高分子酮胺结构。此酮胺结构能在碱性环境中与 硝基四氮唑蓝NBT发生还原反应,生成甲月替,并以果糖胺DMF为标准参照物进行比色反应
产品优点如下:
1、操作简便:单试剂操作,全程约20分钟,可测50例左右样本。
2、再现性好:批内CV=1.3%,批间CV=4.11%。
3、回收试验: X =103%。
4、受外界影响因素小:干扰因素少,重复性强。
所需仪器及自备试剂:
1、糖化血清蛋白(GSP)测定试剂盒(果糖胺法)(带标准)分光光度计/酶标仪/半自动生化分析仪(比色测定波长为530nm)
2、恒温水浴箱或气浴箱 (孵育温度为37℃)
3、漩涡混匀器
4、微量移液器
操作流程:
1、按步骤加入样本和NBT显色剂
2、混匀,37℃水浴15分钟
3、加入稳定剂
4、混匀,530nm波长,1cm光径比色
批间CV 批内CV 回收率 最底检出线 检测范围
3.52 1.7 103 5.0U/ml 5.0-122.1 U/ml
本试剂盒保存期:6个月。贮存温度:2~8℃。特别提醒R1、R2需在-20℃以下冷冻保存。
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