B026 总氨基酸(T-AA)测定试剂盒(比色法) Total Amino Acid assay kit 80管/78样 盒
肝脏、肾脏是氨基酸代谢的主要器管,故尿中氨基酸的变化最能能反应肝、肾的病理、生理状态.另外,氨基酸还能反应灼伤、糖尿病、伤寒等方面情况。
铜离子(Cu++)能与各种氨基酸络合产生蓝绿色的络合物,在一定波长下颜色的深浅与总氨基酸的含量成正比,故可以用可见光分光光度计测其吸光度,通过换算得到总氨基酸含量。
产品优点如下:
1、快速简便:全程约20分钟,可测50例左右样本。
2、稳定性好:试剂盒2~8℃存放6个月有效。
3、再现性好:变异系数CV=1.2%。
4、回收试验: X =96%。
5、总氨基酸(T-AA)测定试剂盒(比色法)受外界影响因素小:干扰因素少,重复性强。
6、测试面广:可测血清(浆)、尿液、药物、食物、饮料、口服液、水产品以及动植物组织中的氨基酸。
所需仪器及自备试剂:
1、分光光度计/酶标仪/半自动生化分析仪(比色测定波长为650nm)
2、台式离心机
3、漩涡混匀器
4、微量移液器
样本收集与前处理:
血清(浆)、尿液样本:可直接测定。
动物组织样本:可用生理盐水按重量体积比制备成组织匀浆液,离心取上清测定。
培养细胞、植物组织:常用PBS作为匀浆介质破碎后离心取上清测定。
具体的样本前处理:参考我们随货发送的《实验方法学》以及试剂盒中详细说明书。
操作流程:
1、按步骤加入样本和反应液
2、漩涡混匀
3、加入显色剂显色,3500转/分离心10分钟
4、取上清, 650nm波长,1cm光径,比色
批间CV 批内CV 回收率 最底检出线 检测范围
5.39 2.3 96 0.5μmol/ml 0-200μmol/ml
本试剂盒保存期:6个月。贮存温度:2~8℃。
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