B009 维生素C(抗坏血酸)(Vitamin C)测定试剂盒(比色法) Vitamin C assay kit (Colorimetric method) 50管/48样 盒
维生素C在体内参与胶原物质合成,是脯氨酸羟化酶和赖氨酸羟化酶的辅因子,是催化多巴胺转变成去甲肾上腺素的铜酶多巴β羟化酶所必需,当维生素C缺乏时,合成的胶原得不到充分的羟化,不能很好的形成纤维。
维生素C最明显的化学活性是作为还原剂,它可以将Fe3+还原成Fe2+,促使铁在肠道吸收,促进铁的贮存和利用。
维生素C(抗坏血酸)(Vitamin C)测定试剂盒(比色法)抗坏血酸作为自由基清除剂,可以很快地与O-2、H2O2反应,更快地与OH-反应生成抗坏血酸自由基,它也可以清除O-2,因此,抗坏血酸可以保护机体免受内源性氧自由基的损伤。
维生素C能与-生育酚自由基还原成维生素E,间接地起着链阻断剂作用。
维生素C是血浆中最有效的抗氧化剂,是细胞外液抗氧化防御系统的第一道防线,Vc对血浆中正在进行着的脂质过氧化作用也有阻断作用。
产品优点如下:
1、快速简便:全程约50分钟,可测100例左右样本。
2、取样量微:本法取样本量约为150l。
3、稳定性好:试剂盒2~8℃存放6个月有效。
4、再现性好:变异系数CV=1.7%。
5、回收试验: X =103.3%。
6、受外界影响因素小:干扰因素少,重复性强。
7、测试面广:可测动物血清(浆)、组织等,效果均佳。
所需仪器及自备试剂:
1、分光光度计/酶标仪/半自动生化分析仪(比色测定波长为536nm)
2、恒温水浴箱或气浴箱 (孵育温度为37℃)
3、台式离心机
4、漩涡混匀器
5、微量移液器
样本收集与前处理:
血清(浆)可直接测定。
动物组织样本:可用生理盐水按重量体积比制备成组织匀浆液,离心取上清测定。
具体的样本前处理:参考我们随货发送的《实验方法学》以及试剂盒中详细说明书。
操作流程:
1、按说明书操作取样150l加R1,3500~4000转/分,离心10分钟,上层清亮液体为上清液
2、加入R2、R3、R4漩涡混匀,37℃孵育30分钟
3、加入R2、R3、R4漩涡混匀,37℃孵育30分钟
4、536nm波长,1cm光径,比色
批间CV 批内CV 回收率 最底检出线 检测范围
4.99 1.5 99 0.2μg/ml 0.2-89.3μg/ml
本试剂盒保存期:6个月。贮存温度:2~8℃。
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