B007-2 过氧化氢酶(CAT)测定试剂盒(紫外法) Catalase (CAT) assay kit (Ultraviolet) 100管/96样
氢氧自由基(OH-)是化学性质最活泼的活性氧,它几乎与细胞内的每一类有机物如糖、氨基酸、磷脂、核苷酸和有机酸等都能反应,并且有非常高的速度常数,因此它的破坏性极强,但它可以被过氧化氢酶分解,因而测定过氧化氢酶的高低就有其重要意义。红细胞或组织中过氧化氢酶(CAT)在一定条件下能直接分解其底物过氧化氢(H2O2),使H2O2在反应液中的浓度逐渐降低,相应的吸光度也逐渐下降。
所需仪器及自备试剂:
1、紫外分光光度计(比色测定波长为240nm)
2、恒温水浴箱或气浴箱 (孵育温度为25℃)
3、漩涡混匀器
4、过氧化氢酶(CAT)测定试剂盒(紫外法)微量移液器
样本收集与前处理:
血清(浆)可直接测定。
红细胞:需按照试剂盒中说明书上红细胞的测定方法进行提取后测定。
动物组织样本:可用生理盐水按重量体积比制备成组织匀浆液,离心取上清测定。
培养细胞、细菌、植物组织:常用PBS作为匀浆介质破碎后离心取上清测定。
具体的样本前处理:参考我们随货发送的《实验方法学》以及试剂盒中详细说明书。
操作流程:
1、20μl样本加底物溶液3ml,240nm处测OD1
2、1分钟后240nm处测OD2
3、根据说明书计算公式得出CAT活力
注:最佳取样量(具体摸索的方法按照试剂盒中说明书要求)
批间CV 批内CV 回收率 最底检出线 检测范围
4.94 1.9 96 0.2U/ml 0.2-24.8U/ml
本试剂盒保存期:6个月。贮存温度:2~8℃
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