过氧化氢酶(CAT)测定试剂盒(紫外法)

B007-2 过氧化氢酶(CAT)测定试剂盒(紫外法) Catalase (CAT) assay kit (Ultraviolet) 100管/96样

氢氧自由基（OH-）是化学性质最活泼的活性氧，它几乎与细胞内的每一类有机物如糖、氨基酸、磷脂、核苷酸和有机酸等都能反应，并且有非常高的速度常数，因此它的破坏性极强，但它可以被过氧化氢酶分解，因而测定过氧化氢酶的高低就有其重要意义。红细胞或组织中过氧化氢酶（CAT）在一定条件下能直接分解其底物过氧化氢（H2O2），使H2O2在反应液中的浓度逐渐降低，相应的吸光度也逐渐下降。

所需仪器及自备试剂：

1、紫外分光光度计（比色测定波长为240nm）

2、恒温水浴箱或气浴箱 （孵育温度为25℃）

3、漩涡混匀器

4、过氧化氢酶(CAT)测定试剂盒(紫外法)微量移液器

样本收集与前处理：

血清（浆）可直接测定。

红细胞：需按照试剂盒中说明书上红细胞的测定方法进行提取后测定。

动物组织样本：可用生理盐水按重量体积比制备成组织匀浆液，离心取上清测定。

培养细胞、细菌、植物组织：常用PBS作为匀浆介质破碎后离心取上清测定。

具体的样本前处理：参考我们随货发送的《实验方法学》以及试剂盒中详细说明书。

操作流程：

1、20μl样本加底物溶液3ml，240nm处测OD1

2、1分钟后240nm处测OD2

3、根据说明书计算公式得出CAT活力

注：最佳取样量（具体摸索的方法按照试剂盒中说明书要求）

批间CV   批内CV   回收率   最底检出线       检测范围

4.94     1.9      96       0.2U/ml          0.2-24.8U/ml

本试剂盒保存期：6个月。贮存温度：2～8℃

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