GN增菌液Gram Negative Enrichment Broth250g主要用于志贺氏菌的增菌培养,亦可用于沙门氏菌增菌培养(GB标准)
GN增菌液
Gram Negative Enrichment Broth
用途:主要用于志贺氏菌的增菌培养,亦可用于沙门氏菌增菌培养(GB标准)
GN增菌液配方:(g/L)
胰蛋白胨 20.0
葡萄糖 1.0
KH2PO4 1.5
磷酸氢二钾 4.0
甘露醇 2.0
枸橼酸钠 5.0
去氧胆酸钠 0.5
NaCl 5.0
pH值7.0 ± 0.1 25℃
原理:
胰蛋白胨提供碳源、氮源、维生素和矿物质;葡萄糖和甘露醇提供糖类;枸缘酸钠和去氧胆酸钠抑制革兰氏阳性细菌,但不影响沙门氏菌和志贺氏菌的生长;KH2PO4和磷酸氢二钾是缓冲剂;NaCl维持均衡的渗透压。
用法:
称取本品39.0g,加热溶解于1000ml 蒸馏水中,分装,121℃高压灭菌 15 分钟,备用。
GN增菌液质量控制:
在36±1℃培养18-24小时。
质控菌株菌株编号生长情况其它特征
福氏志贺氏菌ATCC12022+++浑浊
金黄色葡萄球菌ATCC25923- /
大肠埃希氏菌ATCC25922-/+ /
沙门氏菌ATCC14028+++浑浊
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