人热休克蛋白-70（HSP-70）ELISA试剂盒

Human heat shock protein -70(HSP-70) FOR RESEARCH USE ONLY Assay range：20pg/ml-480pg/ml 96 determinations Purpose This kit allows for the determination of HSP-70 concentrations in Human serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Human HSP-70 level in the sample, use Purified Human HSP-70 antibody to coat microtiter plate wells, make solid-phase antibody, then add HSP-70 to wells, Combined HSP-70 antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human HSP-70 in the samples is then determined by comparing the O.D. of the samples to the standard curve.