人去甲肾上腺素（NA）ELISA试剂盒

Human noradrenaline(NA) Assay range：6 ng/L -200 ng/L 96 determinations Purpose This kit allows for the determination of NA concentrations in Human serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Human NA level in the sample, use Purified Human NA antibody to coat microtiter plate wells, make solid-phase antibody, then add NA to wells, Combined NA antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human NA in the samples is then determined by comparing the O.D. of the samples to the standard curve.