大鼠抗酒石酸酸性磷酸酶5b(TRACP5b)ELISA试剂盒
2015-09-10 16:05
浏览:61次
分享:
资料摘要:
Rat tartrate-resistant acid phosphatase 5b(TRACP5b)
FOR RESEARCH USE ONLY
Assay range:60pg/mL - 1600pg/mL 96 determinations
Purpose
This kit allows for the determination of TRACP5b concentrations in Rat serum, cell culture supernates and other biological fluids
Principle of the assay
The kit assay Rat TRACP5b level in the sample, use Purified Rat TRACP5b antibody to coat microtiter plate wells, make solid-phase antibody, then add TRACP5b to wells, Combined TRACP5b antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat TRACP5b in the samples is then determined by comparing the O.D. of the samples to the standard curve.
下载本篇资料:
大鼠抗酒石酸酸性磷酸酶5b TRACP5b 英文说明书.doc
52KB
相关资料
大鼠三磷酸肌醇(IP3)酶联免疫分析试剂盒用于测定大鼠血清、血浆及相关液体样本中三磷酸肌醇(IP3)含量。
大鼠乳酸脱氢酶(LDH)酶联免疫分析试剂盒用于测定大鼠血清,血浆及相关液体样本中乳酸脱氢酶(LDH)含量。
大鼠乳酸(Lactate)酶联免疫分析试剂盒用于测定大鼠血清,血浆及相关液体样本中乳酸(Lactate)含量。
大鼠葡萄糖(GLU)酶联免疫分析试剂盒用于测定大鼠血清、血浆及相关液体样本中葡萄糖(GLU)含量。本试剂盒仅供研究使用。