大鼠脑钠素（BNP）ELISA试剂盒

Rat BNP FOR RESEARCH USE ONLY Assay range：3μg/L -80μg/L 96 determinations Purpose This kit allows for the determination of BNP concentrations in Rat serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Rat BNP level in the sample, use Purified Rat BNP antibody to coat microtiter plate wells, make solid-phase antibody, then add BNP to wells, Combined BNP antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat BNP in the samples is then determined by comparing the O.D. of the samples to the standard curve.