人α干扰素(IFN-α)检测试剂盒实验操作常见问题分析
异常 | 结果描述 | 原因分析 | 对策 |
白板 | 显色步骤结束后,酶标板所有孔均无颜色。阳性对照不显色。 | 试剂已过效期,或不同试剂盒组分混用 | 检查试剂的组分及批号,确认未过期以及所有组分均属对应的试剂盒。不同试剂盒或不同批号的试剂不能混用。 |
错加、漏加试剂底物、显色剂 A或B | 严格按说明书的人α干扰素(IFN-α)检测试剂盒步骤操作,加完试剂后可观察一下液面高度是否一致,以减少漏加现象。 |
洗板及加样过程中,酶标受污染失活失去催化显色剂显色的能力 | 确认盛酶标的容器不含酶抑制剂如叠氮钠等,确认配制洗液的容器已洗干净。 |
终止液误作洗涤液稀释或当底物缓冲液配制 | 每次配制时都应看清标签标明物质 |
蒸馏水有问题 | 确认配制洗液的纯化水达到要求且未污染,与好蒸馏水比较。 |
标本及采集、贮运因素:严重溶血 ,以 HRP 为标记的ELISA 测定中,残留在孔内的血红蛋白具有过氧化物酶样活性,催化底物显色造成假阳性; 混有红细胞的血清 易沉淀或附着在聚乙烯孔内不易洗净;如有 细菌污染 ,菌体中可能含有内源性HRP,也会产生假阳性反应; 标本凝固不全 ,有时为了争取时间快速检测,常在血液还未开始凝固时即强行离心分离血清,使血清中仍残留部分纤维蛋白原,在ELISA测定过程中可以形成肉眼可见的纤维蛋白块,易造成假阳性结果; 采血试管洗涤不彻底 、反复使用易交叉污染; 塑料试管能吸附抗原物质 ,样本久置在塑料管内会使样本内抗原含量下降造成假阴性。
人α干扰素(IFN-α)检测试剂盒其它相关试剂盒:
xyR152Hu人含卷曲螺旋域蛋白3(CCDC3)ELISA试剂盒ELISA Kit for Coiled Coil Domain Containing Protein 3 (CCDC3)
xyF417Hu人脂肪酸去饱和酶2(FADS2)ELISA试剂盒ELISA Kit for Fatty Acid Desaturase 2 (FADS2)
xyC468Hu人脂肪酸去饱和酶1(FADS1)ELISA试剂盒ELISA Kit for Fatty Acid Desaturase 1 (FADS1)
xyB402Hu人IgA-Fc断片受体(FcaR)ELISA试剂盒ELISA Kit for Fc Fragment Of IgA ReHZptor (FcaR)
xyR751Hu人谷氨酸脱羧酶样蛋白1(GADL1)ELISA试剂盒ELISA Kit for Glutamate Decarboxylase Like Protein 1 (GADL1)
xyF116Hu人肾母细胞瘤蛋白1(WT1)ELISA试剂盒ELISA Kit for Wilms Tumor Protein (WT1)
xyF418Hu人脂肪酸去饱和酶3(FADS3)ELISA试剂盒ELISA Kit for Fatty Acid Desaturase 3 (FADS3)
xyH274Hu人谷氨酰胺酰肽环转移酶(QPCT)ELISA试剂盒ELISA Kit for Glutaminyl Peptide Cyclotransferase (QPCT)
xyP358Hu人无翅型MMTV整合位点家族成员10A(WNT10A)ELISA试剂盒ELISA Kit for Wingless Type MMTV Integration Site Family, Member 10A (WNT10A)
xyP546Hu人无翅型MMTV整合位点家族成员7B(WNT7B)ELISA试剂盒ELISA Kit for Wingless Type MMTV Integration Site Family, Member 7B (WNT7B)
xyP547Hu人无翅型MMTV整合位点家族成员7A(WNT7A)ELISA试剂盒ELISA Kit for Wingless Type MMTV Integration Site Family, Member 7A (WNT7A)
xyP553Hu人无翅型MMTV整合位点家族成员10B(WNT10B)ELISA试剂盒ELISA Kit for Wingless Type MMTV Integration Site Family, Member 10B (WNT10B)
xyB014Hu人整合素关联蛋白(IAP)ELISA试剂盒ELISA Kit for Integrin Associated Protein (IAP)
xyC257Hu人62kDa核孔蛋白(NUP62)ELISA试剂盒ELISA Kit for Nucleoporin 62kDa (NUP62)
xyD115Hu人沙漠刺猬因子(DHH)ELISA试剂盒ELISA Kit for Hedgehog Homolog, Desert (DHH)
xyL534Hu人生长停滞DNA损伤可诱导蛋白α(GADD45α)ELISA试剂盒ELISA Kit for Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a)
xyC775Hu人络丝蛋白(RL)ELISA试剂盒ELISA Kit for Reelin (RL)
xyD372Hu人激肽释放酶12(KLK12)EL人α干扰素(IFN-α)检测试剂盒ISA试剂盒ELISA Kit for Kallikrein 12 (KLK12)
xyD374Hu人激肽释放酶14(KLK14)ELISA试剂盒ELISA Kit for Kallikrein 14 (KLK14)
xyD796Hu人精氨酸酶Ⅱ(Arg2)ELISA试剂盒ELISA Kit for Arginase II (Arg2)
xyD937Hu人肽聚糖识别蛋白1(PGLYRP1)ELISA试剂盒ELISA Kit for Peptidoglycan Recognition Protein 1 (PGLYRP1)
xyF859Hu人分泌粒蛋白Ⅱ(SCG2)ELISA试剂盒ELISA Kit for Secretogranin II (SCG2)
xyJ410Hu人钙调宁蛋白2(CNN2)ELISA试剂盒ELISA Kit for Calponin 2 (CNN2)
xyM347Hu人T-细胞免疫调节因子1(TCIRG1)ELISA试剂盒ELISA Kit for T-HZll, Immune Regulator 1 (TCIRG1)
xyQ201Hu人醛糖还原酶相似蛋白1(ARL1)ELISA试剂盒ELISA Kit for Aldose Reductase Like Protein 1 (ARL1)
xyB250Hu人胞外5'-核苷酸酶(NT5E)ELISA试剂盒ELISA Kit for 5'-Nucleotidase, Ecto (NT5E)
xyB659Hu人胰岛素样生长因子1受体(IGF1R)ELISA试剂盒ELISA Kit for Insulin Like Growth Factor 1 ReHZptor (IGF1R)
xyC021Hu人S100钙结合蛋白A5(S100A5)ELISA试剂盒ELISA Kit for S100 Calcium Binding Protein A5 (S100A5)
xyC477Hu人纤维蛋白原γ(FGγ)ELISA试剂盒ELISA Kit for Fibrinogen Gamma (FGg)