磷酸化白介素-1受体相关激酶4抗体

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英文名称  Anti-phospho-IRAK4(Thr345)

中文名称  磷酸化白介素-1受体相关激酶4抗体

别    名  IL-1 Receptor-associated Kinase 4; 8430405M07Rik; 9330209D03Rik; IPD1; IRAK4; NY-REN-64; REN64; INTERLEUKIN RECEPTOR-ASSOCIATED KINASE 4.

浓    度  1mg/1ml

规 格  0.1ml/100μg    

抗体来源  Rabbit  

克隆类型  polyclonal

交叉反应  Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep

磷酸化白介素-1受体相关激酶4抗体的多样性：

抗体的异质性。抗体的组成极为复杂，是由成千上万、多种多样的免疫球蛋白（Ig）分子所组成。这些Ig分子在形状、大小、结构以及氨基酸的组成和排列上，既相似，又有差别。由于有差别，它们的电泳活性就有很大的变化。

因为抗体具有与抗原决定簇相对应的结合部位（抗原结合簇），所以抗体与抗原的结合具有特异性。另一方面，抗体本身是一种蛋白质，具有本身的氨基酸组成、排列和立体结构，对异种动物来说，它又是抗原。各类Ig都具有可用血清学方法检出的抗原特异性，它们表现出不同的血清学类型。

磷酸化白介素-1受体相关激酶4抗体的相关产品:

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anti-TCF7L2   转录因子7类似物2抗体 0.2ml

产品类型  一抗  磷酸化抗体  

研究领域  细胞生物 免疫学 激酶和磷酸酶  

蛋白分子量  predicted molecular weight: 51kDa

性    状  Lyophilized or Liquid

免 疫 原  KLH conjugated Synthesised phosphopeptide derived from human IRAK4 around the phosphorylation site of Thr345

亚    型  IgG

纯化方法  affinity purified by Protein A

储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500

（石蜡切片需做抗原修复）

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品介绍 Interleukin 1 (IL1) and lipopolysaccharide (LPS) induces cellular responses through IL1 receptor (IL1R) and Toll like receptors (TLR). IL1R associated kinases (IRAK, IRAK2, and IRAKM) regulate the activation of NF-kB and MAP kinase (MAPK) by IL1R/TLR. A novel member in the IRAK/Pelle family was recently identified and designated IRAK4. Overexpression of IRAK4 activates NF-kB and MAPK pathways. IRAK4 interacts with and phosphorylates IRAK1. IRAK4 deficient animals are completely resistant to the challenge with LPS. Animals and humans lacking IRAK4 are impaired in their responses to viral and bacterial challenges. Members in IRAK/Pelle family play a central role in IL1R/TLR mediated inflammatory responses and in innate immunity.

Function : Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.

Subunit : Associates with MYD88 and IRAK2 to form a ternary complex called the Myddosome. Once phosphorylated, IRAK4 dissociates from the receptor complex and then associates with the TNF receptor-associated factor 6 (TRAF6), IRAK1, and PELI1; this intermediate complex is required for subsequent NF-kappa-B activation. Interacts with IL1RL1.

Subcellular Location : Cytoplasm.

Post-translational modifications : Phosphorylated.

DISEASE : Defects in IRAK4 are the cause of recurrent isolated invasive pneumococcal disease type 1 (IPD1) [MIM:610799]. Recurrent invasive pneumococcal disease (IPD) is defined as two episodes of IPD occurring at least 1 month apart, whether caused by the same or different serotypes or strains. Recurrent IPD occurs in at least 2% of patients in most series, making IPD the most important known risk factor for subsequent IPD.

Defects in IRAK4 are the cause of IRAK4 deficiency (IRAK4D) [MIM:607676]. IRAK4 deficiency causes extracellular pyogenic bacterial and fungal infections in otherwise healthy children.

磷酸化白介素-1受体相关激酶4抗体Similarity : Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.

Contains 1 death domain.

Contains 1 protein kinase domain.