胰岛素诱导基因1抗体

以下为您提供的是胰岛素诱导基因1抗体的说明书,了解更多关于胰岛素诱导基因1抗体的英文名称,胰岛素诱导基因1抗体别名,胰岛素诱导基因1抗体规格,请看以下详细介绍.

英文名称  Anti-Insig1

中文名称  胰岛素诱导基因1抗体

别    名  CL 6; CL6; INSIG 1; INSIG 1 membrane protein; INSIG1 membrane protein; Insulin induced gene 1; Insulin induced gene 1 protein; insulin induced gene-1; insulin induced gene1; MGC1405; INSI1_HUMAN.

浓    度  1mg/1ml

规 格  0.2ml/200μg    

抗体来源  Rabbit  

克隆类型  polyclonal

交叉反应  Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit

胰岛素诱导基因1抗体的多样性：

抗体的异质性。抗体的组成极为复杂，是由成千上万、多种多样的免疫球蛋白（Ig）分子所组成。这些Ig分子在形状、大小、结构以及氨基酸的组成和排列上，既相似，又有差别。由于有差别，它们的电泳活性就有很大的变化。

因为抗体具有与抗原决定簇相对应的结合部位（抗原结合簇），所以抗体与抗原的结合具有特异性。另一方面，抗体本身是一种蛋白质，具有本身的氨基酸组成、排列和立体结构，对异种动物来说，它又是抗原。各类Ig都具有可用血清学方法检出的抗原特异性，它们表现出不同的血清学类型。

胰岛素诱导基因1抗体的相关产品:

Anti-TRF1   端粒结合蛋白1抗体 0.2ml

Anti-TrK A   酪氨酸激酶A抗体 0.1ml

Anti-TrK A.B.C   酪氨酸激酶A.B.C抗体 0.2ml

Anti-Trk B   酪氨酸激酶B抗体 0.1ml

Anti-Trk C   酪氨酸激酶C抗体 0.2ml

产品类型  一抗    

研究领域  肿瘤 细胞生物 免疫学 信号转导 细胞凋亡 转录调节因子 糖尿病  

蛋白分子量  predicted molecular weight: 30kDa

性    状  Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human Insig1

亚    型  IgG

纯化方法  affinity purified by Protein A

储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500

（石蜡切片需做抗原修复）

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品介绍 Oxysterols regulate cholesterol homeostasis through liver X receptor (LXR) and sterol regulatory element binding protein (SREBP) mediated signaling pathway. Insig1 is an insulin-induced gene. It encodes an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. Insig1 binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins. It may also play a role in growth and differentiation of tissues involved in metabolic control, and may play a regulatory role during G0/G1 transition of cell growth.

Function : Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs). Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Initiates the sterol-mediated ubiquitin-mediated endoplasmic reticulum-associated degradation (ERAD) of HMGCR via recruitment of the reductase to the ubiquitin ligase, AMFR/gp78. May play a role in growth and differentiation of tissues involved in metabolic control. May play a regulatory role during G0/G1 transition of cell growth.

Subunit : Binds to the SCAP-SREBF2 complex only in the presence of sterols. Interacts with RNF139. Interacts with HMGCR (via its SSD); the interaction, accelerated by sterols, leads to the recruitment of HMGCR to AMFR/gp78 for its ubiquitination by the sterol-mediated ERAD pathway. Interacts with AMFR/gp78 (via its membrane domain); the interaction recruits HMCR at the ER membrane for its ubiquitination and degradation by the sterol-mediated ERAD pathway.

Subcellular Location : Endoplasmic reticulum membrane; Multi-pass membrane protein.

Tissue Specificity : Expressed in all tissues tested with highest expression in the liver.

Post-translational modifications : Ubiquitinated. Subsequent to sterol deprivation, the SCAP-SREBF2 complex becomes dissociated from INSIG1, is then ubiquitinated and degraded in proteasomes. Although ubiquitination is required for rapid INSIG1 胰岛素诱导基因1抗体degradation, it is not required for release of the SCAP-SREBP complex. Ubiquitinated by RNF139.

Similarity : Belongs to the INSIG family.

胰岛素诱导基因1在参与代谢控制的组织生长和分化中发挥作用，在细胞生长的G0/G1期的转变过程中发挥调节作用.