丝氨酸蛋白激酶1抗体

英文名称    CHEK2    

中文名称    丝氨酸蛋白激酶1抗体    

别    名    SPK1; CHEK2 homolog; CHK2 homolog; MEC2; RAD53; CHK2_HUMAN; Serine protein kinase 1; Serine-protein kinase 1; Serine/threonine-protein kinase RAD53; CDS1; CHK2; RAD53.    

丝氨酸蛋白激酶1抗体        

说 明 书    0.2ml      

研究领域    细胞生物  细胞周期蛋白  激酶和磷酸酶  表观遗传学      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    61kDa    

细胞定位    细胞膜     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human Rad53/SPK1    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

丝氨酸蛋白激酶1抗体产品介绍    background:
In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Function:
Controls S-phase checkpoint as well as G1 and G2 DNA damage checkpoints. Phosphorylates proteins on serine, threonine, and tyrosine. Prevents entry into anaphase and mitotic exit after DNA damage via regulation of the Polo kinase CDC5. Seems to be involved in the phosphorylation of RPH1.

Subunit:
Homodimer. Homodimerization is part of the activation process but the dimer may dissociate following activation. Interacts with PML. Interacts with TP53. Interacts with RB1; phosphorylates RB1. Interacts with BRCA1. Interacts (phosphorylated at Thr-68) with MDC1; requires ATM-mediated phosphorylation of CHEK2. Interacts with TP53BP1; modulates CHEK2 phosphorylation at Thr-68 in response to ionizing radiation. Interacts with CDC25A; phosphorylates CDC25A and mediates its degradation in response to ionizing radiation. Interacts with CUL1; mediates CHEK2 ubiquitination and regulation.

Tissue Specificity:
Nucleus.

Post-translational modifications:
Autophosphorylated.

Similarity:
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHEK2 subfamily.
Contains 2 FHA domains.
Contains 1 protein kinase domain.

Database links:

Entrez Gene: 11200 Human

Entrez Gene: 50883 Mouse

Entrez Gene: 114212 Rat

Omim: 604373 Human

SwissProt: O96017 Human

SwissProt: Q9Z265 Mouse

SwissProt: Q9R019 Rat

Unigene: 291363 Human

Unigene: 505297 Human

Unigene: 279308 Mouse

Unigene: 163213 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.    

丝氨酸蛋白激酶1抗体

hz-0652P Connexin-45(CX45)  间隙连接蛋白45(抗原)

hz-0709P Anti-Collagen II  II 型胶原(抗原)

hz-1009P CXCR1 (CXC-chhzokine receptor 1)  细胞表面趋化因子受体1抗原

hz-0732P COX-2 (Prostaglandin-endoperoxide synthase-2)  前列腺素氧化环化酶2(抗原)

hz-0697P c-phycocyanin(C-PC)  C-藻蓝素(藻蓝蛋白)

hz-0743P CTGF (connective tissue growth factor)  结缔组织生长因子(多肽抗原)

hz-0573P Cyclin E  周期素E/原癌基因抗原

hz-0497P Dnmt3a(cytosine-5)-methyltransferase 3A)  DNA甲基转移酶-3α(抗原)

hz-0282P Cyclin C  周期素 C(抗原)

hz-0791P CGRP (calcitonin gene related peptide, CGRP)  降钙素基因相关肽

hz-0614P CULLIN (Cdc53/CUL)  

hz-0461P CYP450(Cytochrome P450 monooxygenase)  细胞色素P450单氧化酶(多肽抗原)

hz-0488P CXorf36(uncharacterized protein Cxorf36 precursor)  脱羧酶蛋白体36 cxorf36抗原

hz-0399P Deltex-1  (DTX1) (Deltex protein 1)抗原

hz-0428P DISC1(disrupted in schizophrenia1)(NT)  DISC1 N端抗原

hz-0430P DISC1(disrupted in schizophrenia1)(CT)  DISC1 C端抗原

hz-0421P DR3/TRAMP (death receptor-3)  DR3 死亡受体（抗原）

hz-0714P E7 protein/HPV 16 (Human papillomavirus type 16)  人乳头瘤病毒16型E7蛋白抗原

hz-0165P EGF-R (Epidhzal growth factor receptor)  表皮生长因子受体（抗原）

hz-0613P Eukaryotic aspartyl protease(Rice)  天冬氨酸蛋白酶家族蛋白(水稻蛋白的一种)（抗原）

hz-0711P FAM3B/PANDER(PANcreatic DERived factor)  一种新型的胰岛细胞因子(抗原)

hz-0788P Fabp2 (fatty acid binding protein 2, intestinal)  脂肪酸结合蛋白2(抗原)

hz-0500P ECG2 (sophagus cancer-related gene-2)  食道癌相关基因（抗原）

hz-0666P Fibronectin (FN)  纤维连结蛋白(多肽抗原)

hz-0734P FGF-7/KGF (Keratinocyte growth factor precursor)  成纤维细胞生长因子-7/纤维母细胞生长因子 (抗原)

hz-0230P FGFR1 peptide  碱性成纤维细胞生长因子受体-1（多肽抗原）

hz-0675P FGFR2(Fibroblast Growth Factor Receptor 2)  成纤维细胞生长因子受体2抗原

hz-0735P FGF-8/HBGF-8 (Fibroblast growth factor 8)  成纤维细胞生长因子-8/纤维母细胞生长因子-8 (抗原)

hz-0400P GAD-65 (glutamate decarboxylase)  谷氨酸脱羧酶-65(C端多肽)

hz-0459P GAPDH(Ribbt Anti-glyceraldhzyde-3-phosphate Dhzydrogease )   3-磷酸甘油醛脱氢酶（抗原）

hz-0721P Galectin-3   半乳糖凝集素-3(抗原)

hz-0701P GCS (glucosylceramide synthase, GCS)  葡萄糖神经酰胺合成酶（抗原）

hz-0639P GFP  绿色荧光蛋白

hz-2194P Recombinant EGFP protein  重组增强型绿色荧光

hz-0725P ER-alpha  雌激素受体alpha