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当前位置: 上海沪峰 > 常用生化试剂 > 5,5'二硫代双(2-硝基苯甲酸)

5,5'二硫代双(2-硝基苯甲酸)

供货周期: 现货
品牌: AMRESCO
CAS号: FK3108
货号: FK3108
报价: 面议
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产品介绍
FK31085,5'二硫代双(2-硝基苯甲酸)98%1g

98%5g

98%25g

98%100g
Cas号69-78-3

MDL:

--MFCD00007140
Beilstein1896821

EINECS:

--200-714-4
分子式C14H8N2O8S2; [-SC6H3(NO2)CO2H]2
分子量396.35
别名5,5'二硫代双(2-硝基苯甲酸) ;双(3-羧基-4-硝基苯基)二硫,6,6ˊ-二硝基-3,3ˊ-二硫代苯甲酸 ;Ellman’s Reagent (DTNB)
分子结构式
备注:






性状Ellman's Reagent (DTNB) reacts with sulfhydryl groups to yield a colored product, providing a reliable method to measure reduced cysteines and other free sulfhydryls in solution.

Ellman's Reagent (5,5'-dithio-bis-[2-nitrobenzoic acid]) is used to estimate sulfhydryl groups in a sample by comparing to a standard curve of a sulfhydryl-containing compound such as cysteine. Alternatively, sulfhydryl groups can be assayed using the extinction coefficient of TNB (14,150M-1cm-1 at 412nm). Ellman's Reagent has been used for applications other than sulfhydryl estimation, such as for determining alkylthiols by HPLC using a pre-column derivatization procedure and to study thiols in the active site of several enzymes.

Highlights:
?Sulfhydryl assay – reacts quantitatively with free (reduced) sulfhydryl groups (–SH) to yield detectable TNB product
?Colorimetric detection – colored product enables spectrophotometric measurement in cuvettes or microplates (λmax = 412nm; ε = 14,150/M?cm)
?Proven method – well-characterized chemistry provides quantitation of peptide or protein sulfhydryl groups using extinction coefficient calculation or by comparison to a cysteine standard

Ellman's Reagent (DTNB) reacts with sulfhydryl groups to yield a colored product, providing a reliable method to measure reduced cysteines and other free sulfhydryls in solution. Ellman's Reagent (5,5'-dithio-bis-[2-nitrobenzoic acid]) is used to estimate sulfhydryl groups in a sample by comparing to a standard curve of a sulfhydryl-containing compound such as cysteine. Alternatively, sulfhydryl groups can be assayed using the extinction coefficient of TNB (14,150M-1cm-1 at 412nm). Ellman's Reagent has been used for applications other than sulfhydryl estimation, such as for determining alkylthiols by HPLC using a pre-column derivatization procedure and to study thiols in the active site of several enzymes. Highlights: ?Sulfhydryl assay – reacts quantitatively with free (reduced) sulfhydryl groups (–SH) to yield detectable TNB product ?Colorimetric detection – colored product enables spectrophotometric measurement in cuvettes or microplates (λmax = 412nm; ε = 14,150/M?cm) ?Proven method – well-characterized chemistry provides quantitation of peptide or protein sulfhydryl groups using extinction coefficient calculation or by comparison to a cysteine standard
备注:以上描述,仅作参考,并不表示您收到的产品,与此项描述完全相符,产品的出厂标准,请参见质量标准。
贮存阴凉干燥处保存
用途巯基测定,是测定蛋白质、多肽和组织中巯基的灵敏试剂。也用于有机磷农药中毒的监测(胆碱酯酶测定)。


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上海沪峰化工有限公司

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310112000876111

成立日期

2009-03-09

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50

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5,5'二硫代双(2-硝基苯甲酸)由上海沪峰化工有限公司为您提供,货号FK3108,规格:,CAS号:FK3108,如您想了解更多关于5,5'二硫代双(2-硝基苯甲酸)价格、5,5'二硫代双(2-硝基苯甲酸)结构式、批发、用途等信息,欢迎咨询。除供应5,5'二硫代双(2-硝基苯甲酸)外,还可为您提供L-(-)-甘露糖、N-甲基吗啉-N-氧化物、麦芽七糖等试剂,公司有专业的客户服务团队,是您值得信赖的合作伙伴,上海沪峰客户服务电话,售前、售后均可联系。

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