去整合素样金属蛋白酶12

英文名称  Anti-ADAM12/MLTN 

中文名称  去整合素样金属蛋白酶12 

别    名  A disintegrin and metalloproteinase domain 12; A disintegrin and metalloproteinase domain 12; ADA12_HUMAN; ADAM 12; ADAM metallopeptidase domain 12; ADAM12; Disintegrin and metalloproteinase domain-containing protein 12; MCMP; MCMPMltna; MCMPMltna; Meltrin alpha; Meltrin-alpha; MLTN; MLTNA; OTTHUMP00000046766. 

浓    度  1mg/1ml 

规 格  0.2ml/200μg  

抗体来源  Rabbit  

克隆类型  polyclonal 

去整合素样金属蛋白酶12交叉反应  Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit   

产品类型  一抗    

研究领域  肿瘤 细胞生物 免疫学 信号转导 细胞粘附分子 细胞骨架 细胞外基质 泛素 

蛋白分子量  predicted molecular weight: 77kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated synthetic peptide derived from human ADAM12/MLTN 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 

产品应用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  Flow-Cyt=1:100-500  ICC=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 癈 for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20癈. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 癈. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

去整合素样金属蛋白酶12产品介绍 ADAM (a disintegrin and metalloprotease) proteins are a family of over 30 membrane-anchored, glycosylated, Zn2+ dependent proteases that are involved in cell-cell, cell-matrix interface related processes including fertilization, muscle fusion, secretion of TNF?(tumor necrosis factor ?, and modulation of the neurogenic function of Notch and Delta (1-3). ADAM proteins possess a signal-domain, a pro-domain, a metalloprotease domain, a disintegrin domain (Integrin ligand), a cysteine-rich region, an epidermal growth factor-like domain, a transmembrane domain and a cytoplasmic tail (1-3). ADAMs are expressed in brain, testis, epididymis, ovary, breast, placenta, liver, heart, lung, bone, and muscle, and catalyze proteolysis, adhesion, fusion, and intracellular signaling (3). ADAM 12 (Meltrin-a) is produced as 2 differentially spliced isoforms, a 718 amino acid secreted form (ADAM12S) and a 881 amino acid membrane-bound form (ADAM12L), and is involved in egg-sperm fusion (4-6). 

Function : Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors (By similarity). 

Subunit : Interacts with alpha-actinin-2 and with syndecans (By similarity). Interacts with SH3PXD2A. Interacts with FST3. Interacts with GNB2L1/RACK1; the interaction is required for PKC-dependent translocation of ADAM12 to the cell membrane. 

Subcellular Location : Isoform 1: Cell membrane; Single-pass type I membrane protein.

Isoform 2: Secreted.

Isoform 3: Secreted (Potential).

Isoform 4: Secreted (Potential).

Tissue Specificity : Isoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas. 

Post-translational modifications : The precursor is cleaved by a furin endopeptidase (By similarity). 

Similarity : Contains 1 disintegrin domain.

Contains 1 EGF-like domain.

Contains 1 peptidase M12B domain. 

Database links : UniProtKB/Swiss-Prot: O43184.3