α增强子结合蛋白1抗体

英文名称  Anti-ATBF1 

中文名称  α增强子结合蛋白1抗体 

别    名  Alpha fetoprotein enhancer binding protein; AT binding transcription factor 1; AT motif binding factor; ATBT; ZFHX3; ZFHX3_HUMAN. 

浓    度  1mg/1ml 

规 格  0.2ml/200μg  

抗体来源  Rabbit  

克隆类型  polyclonal 

交叉反应  Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit, Sheep   

产品类型  一抗    

研究领域  肿瘤 神经生物学 转录调节因子 锌指蛋白 表观遗传学  

α增强子结合蛋白1抗体蛋白分子量  predicted molecular weight: 404kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated synthetic peptide derived from human ATBF1 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 

产品应用   ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

α增强子结合蛋白1抗体产品介绍 AT-motif binding factor 1 (ATBF1) binds to the AT-rich core sequence element in the human a-fetoprotein enhancer (1). Alternative splicing generates the ATBF1-A and ATBF1-B (2,3). While ATBF1-A contains a 920-amino acid extension at the N-terminus, both ATBF1-A and ATBF1-B contain 4 DNA-binding homeobox domains (2,3). Additionally, ATBF1-A contains 23 zinc finger motifs while ATBF1-B contains 18 zinc finger motifs (1–3). The N-terminal extension unique to ATBF1-A has transcriptional repressor activity (4). In the small intestine, ATBF1-A inhibits expression of the brushborder enzyme aminopeptidase-N through direct binding to the AT motif element (5). Besides functioning in transcription regulation, ATBF1 also functions in ATPase activity (6). ATPase activity associated with ATBF1-A is DNA/RNA-dependent and requires both homeobox domains and zinc finger motifs (6). ATBF1 is highly expressed in spleen and brain tissues (7). The gene encoding human ATBF1 maps to chromosome 16q22.3-q23.1 (8).

Function : ATBF1 is a transcription factor that negatively regulates alpha fetoprotein and MYB, transactivates CDKN1A, and may be a tumor suppressor. Loss of ATBF1 is one mechanism that defines the absence of growth control in prostate cancer. Presence of the isoform ABTF1-A is correlated with better prognosis in breast cancer and may also serve as a marker of endocrine responsiveness. 

Subunit : Interacts with FNBP3 (By similarity). Interacts with PIAS3.

Subcellular Location : Nuclear

Tissue Specificity : Not found in normal gastric mucosa but found in gastric carcinoma cells (at protein level).

Post-translational modifications : Phosphorylated upon DNA damage, probably by ATM or ATR.

Similarity : Contains 22 C2H2-type zinc fingers.

Contains 4 homeobox DNA-binding domains. 

Database links : UniProtKB/Swiss-Prot: Q15911.2