α1,4-N-乙酰葡糖胺转移酶α4Gn-T抗体

英文名称  Anti-A4GNT 

中文名称  α1,4-N-乙酰葡糖胺转移酶α4Gn-T抗体 

别    名  4-N-acetylglucosaminyltransferase; A4GCT_HUMAN; A4GNT; Alpha 1 4 N acetylglucosaminyltransferase; Alpha-1; Alpha4GnT; EC 2.4.1.; MGC149493. 

浓    度  1mg/1ml 

规 格  0.2ml/200μg

抗体来源  Rabbit  

克隆类型  polyclonal 

交叉反应  Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep   

产品类型  一抗    

α1,4-N-乙酰葡糖胺转移酶α4Gn-T抗体研究领域  肿瘤 细胞生物 信号转导  

蛋白分子量  predicted molecular weight: 39kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated synthetic peptide derived from human A4GNT (131-180aa) 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 

产品应用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 癈 for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20癈. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 癈. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

α1,4-N-乙酰葡糖胺转移酶α4Gn-T抗体产品介绍 Alpha 1,4-N-acetylflucosaminyltransferase (Alpha4Gn-T) mediates the biosynthesis of mucin type glycoprotein (O-glycan). Alpha4Gn-T acts as the key enzyme for the formation of the unique glycan GlcNAcalpha1-4Galbeta1-R, and most efficiently transfers N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans. Alpha4Gn-T is a single-pass type II membrane protein associated with the Golgi apparatus and contains the conserved DXD motif involved in catalytic activity. It is expressed in stomach and pancreas, as well as in gastric cancer cells. Alpha4Gn-T is not expressed in peripheral blood cells, making it a useful biomarker for pancreatic cancer. Alpha4Gn-T and Mucin 6 expression is upregulated in the gastric mucosa of H.pylori infected patients, which suggest the involvement of ?Gn-T in defense against H. pylori infection.

Function : Necessary for the synthesis of type III mucin. Catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans.

Subcellular Location : Golgi apparatus membrane.

Tissue Specificity : Detected in stomach and pancreas.

Similarity : Belongs to the glycosyltransferase 32 family.

Database links : UniProtKB/Swiss-Prot: Q9UNA3.1