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轴抑制蛋白2抗体

供货周期：一周

品牌：

规格： 0.2ml/200μg

货号：

CAS号：

报价： ¥1

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产品介绍

轴抑制蛋白2抗体勿使用无霜冷冻箱，因为会冷冻循环，这样会损伤抗体。

英文名称 Anti-Axin 2

中文名称轴抑制蛋白2抗体

别名 Axil; Axin like protein; Axin-2; Axin-like protein; Axin2; Axis inhibition protein 2; AXIN2_HUMAN; Conductin.

浓度 1mg/1ml

规格 0.2ml/200μg

抗体来源 Rabbit

克隆类型 polyclonal

交叉反应 Human, Mouse, Rat, Dog, Horse

轴抑制蛋白2抗体产品类型一抗

研究领域肿瘤细胞生物免疫学神经生物学信号转导干细胞细胞周期蛋白转录调节因子

蛋白分子量 predicted molecular weight: 93kDa

性状 Lyophilized or Liquid

免疫原 KLH conjugated synthetic peptide derived from human Axin 2 corresponding to amino acids 756-795

亚型 IgG

纯化方法 affinity purified by Protein A

储存液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

轴抑制蛋白2抗体产品介绍 Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B.

Involvement in disease:Defects in AXIN2 are involved in colorectal cancer (CRC). They appear to be specifically associated with defective mismatch repair.

Defects in AXIN2 are the cause of oligodontia-colorectal cancer syndrome (ODCRCS). Affected individuals manifest severe tooth agenesis and colorectal cancer or precancerous lesions of variable types.

Function : Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B (By similarity).

Subunit : Interacts with glycogen synthase kinase-3 beta (GSK3B) and beta-catenin. The interaction between axin and beta-catenin occurs via the armadillo repeats contained in beta-catenin (By similarity). Interacts with SMAD7 and RNF111. Interacts with ANKRD6.

Subcellular Location : Cytoplasm.

Tissue Specificity : Expressed in brain and lymphoblast.

Post-translational modifications : Probably phosphorylated by GSK3B and dephosphorylated by PP2A (By similarity).

ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination and subsequent activation of the Wnt signaling pathway.

Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription.

DISEASE : Defects in AXIN2 are involved in colorectal cancer (CRC) [MIM:114500]. They appear to be specifically associated with defective mismatch repair.

Defects in AXIN2 are the cause of oligodontia-colorectal cancer syndrome (ODCRCS) [MIM:608615]. Affected individuals manifest severe tooth agenesis and colorectal cancer or precancerous lesions of variable types.

Similarity : Contains 1 DIX domain.

Contains 1 RGS domain.

Database links : UniProtKB/Swiss-Prot: Q9Y2T1.1

我们应该如何储存抗体且最大限度地提高保质期，简单地说：

抗体应在（-20℃）中冷冻干燥或浓缩的液体形式，直到需要，避免反复冷冻/解冻周期，降低抗体含量和浓度。相反，冻结在单次使用的等分试样重组抗体或添加防冻剂（甘油或乙二醇），从而使料液可以保持在-20℃下为液体形式，以使移液没有“解冻”。

在4℃下存放，避免抗体（或其他蛋白质溶液）超过几天浓缩存量（> 0.5毫克）通常是稳定的，轴抑制蛋白2抗体微生物添加剂，如叠氮化钠。不要试图保存稀释（<0.1mg/ml时）抗体溶液稳定，除非通过添加“载体”蛋白质或特殊添加剂。