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当前位置: 上海基免 > 抗体/抗原 > 腺病毒早期E1A蛋白抗体

腺病毒早期E1A蛋白抗体

供货周期: 7天
品牌:
型号: 0.2ml/200μg
货号:
报价: ¥1
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产品介绍
腺病毒早期E1A蛋白抗体勿使用无霜冷冻箱,因为会冷冻循环,这样会损伤抗体。
英文名称  Anti-Adenovirus 5 E1A 
中文名称  腺病毒早期E1A蛋白抗体 
别    名  Adenovirus early E1A protein; Control protein E1A; E1A; Early E1A protein; Human adenovirus type 5; Human adenovirus C; pfam02703; E1A_ADE05. 
浓    度  1mg/1ml 
规 格  0.2ml/200μg
抗体来源  Rabbit  
克隆类型  polyclonal 
交叉反应  adenovirus5  
产品类型  一抗    
腺病毒早期E1A蛋白抗体研究领域  免疫学 细菌及病毒  
蛋白分子量  predicted molecular weight: 32kDa 
性    状  Lyophilized or Liquid 
免 疫 原  KLH conjugated synthetic peptide derived from human Adenovirus 5 E1A 
亚    型  IgG 
纯化方法  affinity purified by Protein A 
储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide 
产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500 
(石蜡切片需做抗原修复) 
 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user.  
保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 
Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
腺病毒早期E1A蛋白抗体产品介绍 The early region (E1) of the adenovirus genome, responsible for transforming activity, is localized within the left most 11% of the viral genome and consists of two transcriptional units E1A and E1B. E1A is sufficient for partial transformation and immortalization of primary cells. E1A gene products are necessary for normal levels of transcription of the other early regions of the adenovirus genome during productive infection and are able to either activate or repress the transcription of specific cellular genes. E1A forms specific complexes with cellular proteins including p105 causing inhibition of the cell cycle inducing arresting function of p105.
Function : E1A protein has both transforming and trans-activating activities. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Disrupts the function of host retinoblastoma protein RB1/pRb and isoform early E1A 26 kDa protein stabilizes TP53, which are key regulators of the cell cycle. Induces the disassembly of the E2F1 transcription factors from RB1 by direct competition for the same binding site on RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Inactivation of the ability of RB1 to arrest the cell cycle is critical for cellular transformation, uncontrolled cellular growth and proliferation induced by viral infection. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. Interaction with RBX1 and CUL1 inhibits ubiquitination of the proteins targeted by SCF(FBW7) ubiquitin ligase complex, and may be linked to unregulated host cell proliferation. The tumorigenesis-restraining activity of E1A may be related to the disruption of the host CtBP-CtIP complex through the CtBP binding motif.
Subunit : Interacts with host UBE2I; this interaction interferes with polySUMOylation (Probable). Interaction with host RB1 induces the aberrant dissociation of RB1-E2F1 complex thereby disrupting RB1's activity. Interacts with host ATF7; the interaction enhances ATF7-mediated viral transactivation activity which requires the zinc binding domains of both proteins. Isoform early E1A 32 kDa protein and isoform early E1A 26 kDa protein interact (via N-terminus) with CUL1 and E3 ubiquitin ligase RBX1; these interactions inhibit RBX1-CUL1-dependent elongation reaction of ubiquitin chains. Interacts (via N-terminus) with the SCF(FBW7) complex; this interaction results in inhibition of the ubiquitin ligase activity of the SCF(FBW7) complex, ubiquitination of SCF(FBW7) target proteins being diminished. Interacts (via PXLXP motif) with host ZMYND11/BS69 (via MYND-type zinc finger); this interaction inhibits E1A mediated transactivation.
Subcellular Location : Host nucleus (Potential).
Similarity : Belongs to the adenoviridae E1A protein family.
Database links : UniProtKB/Swiss-Prot: P03255.1
我们应该如何储存抗体且最大限度地提高保质期,简单地说:
抗体应在(-20℃)中冷冻干燥或浓缩的液体形式,直到需要,避免反复冷冻/解冻周期,降低抗体含量和浓度。相反,冻结在单次使用的等分试样重组抗体或添加防冻剂(甘油或乙二醇),从而使料液可以保持在-20℃下为液体形式,以使移液没有“解冻”。
在4℃下存放,避免抗体(或其他蛋白质溶液)超过几天浓缩存量(> 0.5毫克)通常是稳定的,腺病毒早期E1A蛋白抗体微生物添加剂,如叠氮化钠。不要试图保存稀释(<0.1mg/ml时)抗体溶液稳定,除非通过添加“载体”蛋白质或特殊添加剂。
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上海基免实业有限公司

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310116002801941

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2013-03-11

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腺病毒早期E1A蛋白抗体由上海基免实业有限公司为您提供,货号,规格:0.2ml/200μg ,CAS号:,如您想了解更多关于腺病毒早期E1A蛋白抗体价格、腺病毒早期E1A蛋白抗体结构式、批发、用途等信息,欢迎咨询。除供应腺病毒早期E1A蛋白抗体外,还可为您提供安普霉素(Apramycin)检测试剂盒、植物S-鸟氨酸转氨酶(OTC)检测试剂盒、微囊藻毒素(MC)检测试剂盒等试剂,公司有专业的客户服务团队,是您值得信赖的合作伙伴,上海基免客户服务电话,售前、售后均可联系。
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