自噬相关蛋白18抗体

供货周期： 7天

品牌：

型号： 0.2ml/200μg

货号：

报价： ¥1

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产品介绍

自噬相关蛋白18抗体勿使用无霜冷冻箱，因为会冷冻循环，这样会损伤抗体。

英文名称 Anti-ATG18/WIPI1

中文名称自噬相关蛋白18抗体

别名 ATG 18; ATG18; Atg18 protein homolog; ATG18A; WD repeat domain phosphoinositide interacting 1; WD repeat domain phosphoinositide interacting protein 1; WD40 repeat protein interacting with phosphoInositides of 49kDa; WIPI 1 alpha; WIPI 1; WIPI 49; WIPI 49 kDa ; WIPI49.

浓度 1mg/1ml

规格 0.2ml/200μg

抗体来源 Rabbit

克隆类型 polyclonal

交叉反应 Human, Mouse, Rat, Pig

自噬相关蛋白18抗体产品类型一抗

研究领域肿瘤细胞生物免疫学细胞凋亡转录调节因子

蛋白分子量 predicted molecular weight: 49kDa

性状 Lyophilized or Liquid

免疫原 KLH conjugated synthetic peptide derived from mouse WIPI1

亚型 IgG

纯化方法 affinity purified by Protein A

储存液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

自噬相关蛋白18抗体产品介绍 Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI1 (WD repeat domain, phosphoinositide interacting 1), have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids. WIPI1 moved through the same set of endosomal membranes as that followed by mannose-6-phosphate receptor (MPR). Consistent with this, WIPI1 was enriched in clathrin-coated vesicles. Overexpression of WIPI1 disrupted the function of the MPR pathway, whereas expression of a double point mutant unable to bind phosphoinositides had no effect. Suppression of WIPI1 by interfering RNA indicated that WIPI1 is required for normal endosomal organization and distribution of IGF2R.

我们应该如何储存抗体且最大限度地提高保质期，简单地说：

抗体应在（-20℃）中冷冻干燥或浓缩的液体形式，直到需要，避免反复冷冻/解冻周期，降低抗体含量和浓度。相反，冻结在单次使用的等分试样重组抗体或添加防冻剂（甘油或乙二醇），从而使料液可以保持在-20℃下为液体形式，以使移液没有“解冻”。

在4℃下存放，避免抗体（或其他蛋白质溶液）超过几天浓缩存量（> 0.5毫克）通常是稳定的，自噬相关蛋白18抗体微生物添加剂，如叠氮化钠。不要试图保存稀释（<0.1mg/ml时）抗体溶液稳定，除非通过添加“载体”蛋白质或特殊添加剂。