英文名称 Anti-ASAP1/DDEF1
中文名称 发育分化增强因子1抗体
别 名 AMAP 1; AMAP1; AMAP-1; ARF GTPase activating protein 1; ASAP 1; Centaurin beta 4; CentB4; DDEF 1; DDEF1 protein; PAG 2; PAG2; PAG-2; PAP; PIP2 dependent ARF1 GAP; ZG 14P; ZG14P; Development and differentiation enhancing factor 1; 130 kDa phosphatidylinositol 4 5 biphosphate dependent ARF1 GTPase activating protein; ADP ribosylation factor directed GTPase activating protein 1; ASAP1_HUMAN.
浓 度 1mg/1ml
规 格 0.1ml/100μg 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Dog, Cow, Horse, Rabbit
发育分化增强因子1抗体产品类型 一抗
研究领域 免疫学 信号转导 转录调节因子
蛋白分子量 predicted molecular weight: 125kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human ASAP1 (154-190aa)
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
发育分化增强因子1抗体产品介绍 ADP-ribosylation factor (Arf) family of small GTP-binding proteins plays a central role in membrane trafficking and cytoskeletal remodeling. ASAP1 (Arf-GAP containing SH3, ankyrin repeats and PH domain) is a phospholipid-dependent Arf GTPase-activating protein that binds to the protein-tyrosine kinases Src and focal adhesion kinase. ASAP1 may act as an oncogene in cancer, and it may be a useful diagnostic marker and therapeutic target.
Function : Possesses phosphatidylinositol 4,5-bisphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types (By similarity). Plays a role in ciliogenesis.
Subunit : Homodimer. Interacts with SRC and CRK. Interacts with RAB11FIP3. Interacts with PTK2B/PYK2. Interacts with CTTN. Interacts (via SH3 domain) with APC.
Subcellular Location : Cytoplasm. Membrane. Note=Predominantly cytoplasmic. Partially membrane-associated
Post-translational modifications : Phosphorylated on tyrosine residues by SRC
Similarity : Contains 2 ANK repeats.
Contains 1 Arf-GAP domain.
Contains 1 PH domain.
Contains 1 SH3 domain.
Database links : UniProtKB/Swiss-Prot: Q9ULH1.4
抗体应在(-20℃)中冷冻干燥或浓缩的液体形式,直到需要,避免反复冷冻/解冻周期,降低抗体含量和浓度。相反,冻结在单次使用的等分试样重组抗体或添加防冻剂(甘油或乙二醇),从而使料液可以保持在-20℃下为液体形式,以使移液没有“解冻”。