生长停滞特异性蛋白6抗体

生长停滞特异性蛋白6抗体英文名称  Anti-GAS 6 

中文名称  生长停滞特异性蛋白6抗体 

别    名  AXL receptor tyrosine kinase ligand; AXL stimulatory factor; AXLLG; AXS F; AXSF; Gas 6; GAS6; Growth-arrest-specific protein 6; Growth-arrest-specific protein 6 precursor. 

浓    度  1mg/1ml 

规 格  0.2ml/200μg     

纯化的抗体可通过不同的途径获取，有些生长停滞特异性蛋白6抗体可通过下述方法制备或从商家购买。从商家购买的抗体，通常附有正确的储存方法。

1）工作液应在4℃下融化并存放，可能稳定达数月。

2）如果没有特殊原因而避免使用叠氮钠，亦可加入叠氮钠，浓度为0．02％。将纯化的抗体样本分装成合适的体积，于-20℃保存。

3）纯化的抗体溶液应以较高的浓度(如lmg／m1)在中性pH下保存。：常用的抗体储存浓度高达l0mg/ml。较低浓度的抗体冻存前应浓缩。所有标准的浓缩方法(如超滤法)，皆可使用。还有一个简单的方法是用蛋白A或蛋白G亲和柱来浓缩溶液。如果纯化的抗体不是用于标记，可将它们以较低浓度储存于加有1％BSA的溶液中。

4）经纯化制备的抗体在常用的缓冲液中是稳定的。其DH应保持在中性左右。如果pH在7-8之间，即使保存多年，对抗体也无损害。多数情况下，盐浓度适于保持在0-150mmol/L之间，但在长期存放的抗体中，盐溶液浓度高达500mmol/L时，对生长停滞特异性蛋白6抗体能有损害。如果没有其他说明．律议用PBS或50mmol/LTris(DH8．0)溶液长期存放抗体。  

抗体来源  Rabbit  

克隆类型  polyclonal 

交叉反应  Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse

产品类型  一抗    

研究领域  肿瘤 心血管 信号转导 干细胞 细胞凋亡 转录调节因子 激酶和磷酸酶  

蛋白分子量  predicted molecular weight: 76kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated synthetic peptide derived from human GAS 6 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 

产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

生长停滞特异性蛋白6抗体产品介绍 GAS 6 is a ligand for the tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS 6 regulates chondrogenic differentiation from mesenchymal cells and also plays a role in thrombosis by amplifying platelet aggregation and secretion in response to known agonists. It is over expressed in human gliomas and regulate the phagocytic function of sertoli cells.

Function : Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses.

Subunit : Heterodimer and heterotetramer with AXL.

Subcellular Location : Secreted.

Tissue Specificity : Plasma. Isoform 1 and isoform 2 are widely expressed. Isoform 1 is the predominant form in spleen.

Post-translational modifications : Isoform 1 is proteolytically processed after secretion to yield a N-terminal 36 kDa protein and a C-terminal 50 kDa protein including the laminin G-like domains which activates AXL. 

Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.

Similarity : Contains 4 EGF-like domains.

Contains 1 Gla (gamma-carboxy-glutamate) domain.

Contains 2 laminin G-like domains.

Database links : UniProtKB/Swiss-Prot: Q14393.2

GAS6在基因转录调节中起重要的调节作用,参与对细胞存活、增殖、粘附、迁移及抗凋亡的调节，Axl属于受体酪氨酸激酶(receptor tyrosine kinases,RTK)家族成员,为Axl的生物配体。经研究发现；Gas6与多种肿瘤细胞的增殖、存活、血管发生、侵袭有关，Gas6系统具有广泛的生物学作用，Gas6属维生素K依赖的蛋白质,广泛表达于各类细胞。