生长停滞特异性蛋白6抗体英文名称 Anti-GAS 6
中文名称 生长停滞特异性蛋白6抗体
别 名 AXL receptor tyrosine kinase ligand; AXL stimulatory factor; AXLLG; AXS F; AXSF; Gas 6; GAS6; Growth-arrest-specific protein 6; Growth-arrest-specific protein 6 precursor.
浓 度 1mg/1ml
规 格 0.2ml/200μg
纯化的抗体可通过不同的途径获取,有些生长停滞特异性蛋白6抗体可通过下述方法制备或从商家购买。从商家购买的抗体,通常附有正确的储存方法。
1)工作液应在4℃下融化并存放,可能稳定达数月。
2)如果没有特殊原因而避免使用叠氮钠,亦可加入叠氮钠,浓度为0.02%。将纯化的抗体样本分装成合适的体积,于-20℃保存。
3)纯化的抗体溶液应以较高的浓度(如lmg/m1)在中性pH下保存。:常用的抗体储存浓度高达l0mg/ml。较低浓度的抗体冻存前应浓缩。所有标准的浓缩方法(如超滤法),皆可使用。还有一个简单的方法是用蛋白A或蛋白G亲和柱来浓缩溶液。如果纯化的抗体不是用于标记,可将它们以较低浓度储存于加有1%BSA的溶液中。
4)经纯化制备的抗体在常用的缓冲液中是稳定的。其DH应保持在中性左右。如果pH在7-8之间,即使保存多年,对抗体也无损害。多数情况下,盐浓度适于保持在0-150mmol/L之间,但在长期存放的抗体中,盐溶液浓度高达500mmol/L时,对生长停滞特异性蛋白6抗体能有损害。如果没有其他说明.律议用PBS或50mmol/LTris(DH8.0)溶液长期存放抗体。
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse
产品类型 一抗
研究领域 肿瘤 心血管 信号转导 干细胞 细胞凋亡 转录调节因子 激酶和磷酸酶
蛋白分子量 predicted molecular weight: 76kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human GAS 6
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
生长停滞特异性蛋白6抗体产品介绍 GAS 6 is a ligand for the tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS 6 regulates chondrogenic differentiation from mesenchymal cells and also plays a role in thrombosis by amplifying platelet aggregation and secretion in response to known agonists. It is over expressed in human gliomas and regulate the phagocytic function of sertoli cells.
Function : Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses.
Subunit : Heterodimer and heterotetramer with AXL.
Subcellular Location : Secreted.
Tissue Specificity : Plasma. Isoform 1 and isoform 2 are widely expressed. Isoform 1 is the predominant form in spleen.
Post-translational modifications : Isoform 1 is proteolytically processed after secretion to yield a N-terminal 36 kDa protein and a C-terminal 50 kDa protein including the laminin G-like domains which activates AXL.
Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
Similarity : Contains 4 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 2 laminin G-like domains.
Database links : UniProtKB/Swiss-Prot: Q14393.2
GAS6在基因转录调节中起重要的调节作用,参与对细胞存活、增殖、粘附、迁移及抗凋亡的调节,Axl属于受体酪氨酸激酶(receptor tyrosine kinases,RTK)家族成员,为Axl的生物配体。经研究发现;Gas6与多种肿瘤细胞的增殖、存活、血管发生、侵袭有关,Gas6系统具有广泛的生物学作用,Gas6属维生素K依赖的蛋白质,广泛表达于各类细胞。