葡萄糖调节蛋白78抗体(热休克蛋白70蛋白5)英文名称 Anti-GRP78/BIP/HSPA5
中文名称 葡萄糖调节蛋白78抗体(热休克蛋白70蛋白5)
别 名 glucose regulated protein 78; 78 kDa glucose regulated protein; BIP; Endoplasmic reticulum lumenal Ca2+ binding protein grp78; FLJ26106; Glucose Regulated Protein 78kDa; GRP 78; GRP78; Heat Shock 70kDa Protein 5; HSPA 5; HSPA-5; HSPA5; Immunoglobulin Heavy Chain Binding Protein; MIF2; GRP78_HUMAN.
浓 度 1mg/1ml
规 格 0.1ml/100μg 0.2ml/200μg
纯化的抗体可通过不同的途径获取,有些葡萄糖调节蛋白78抗体(热休克蛋白70蛋白5)可通过下述方法制备或从商家购买。从商家购买的抗体,通常附有正确的储存方法。
1)工作液应在4℃下融化并存放,可能稳定达数月。
2)如果没有特殊原因而避免使用叠氮钠,亦可加入叠氮钠,浓度为0.02%。将纯化的抗体样本分装成合适的体积,于-20℃保存。
3)纯化的抗体溶液应以较高的浓度(如lmg/m1)在中性pH下保存。:常用的抗体储存浓度高达l0mg/ml。较低浓度的抗体冻存前应浓缩。所有标准的浓缩方法(如超滤法),皆可使用。还有一个简单的方法是用蛋白A或蛋白G亲和柱来浓缩溶液。如果纯化的抗体不是用于标记,可将它们以较低浓度储存于加有1%BSA的溶液中。
4)经纯化制备的抗体在常用的缓冲液中是稳定的。其DH应保持在中性左右。如果pH在7-8之间,即使保存多年,对抗体也无损害。多数情况下,盐浓度适于保持在0-150mmol/L之间,但在长期存放的抗体中,盐溶液浓度高达500mmol/L时,对葡萄糖调节蛋白78抗体(热休克蛋白70蛋白5)能有损害。如果没有其他说明.律议用PBS或50mmol/LTris(DH8.0)溶液长期存放抗体。
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat
产品类型 一抗
研究领域 肿瘤 细胞生物 免疫学
蛋白分子量 predicted molecular weight: 78kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human GRP78 C-terminus
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
葡萄糖调节蛋白78抗体(热休克蛋白70蛋白5)产品介绍 The 78 kDa glucose regulated protein/BiP (GRP78) belongs to the family of ~70 kDa heat shock proteins (HSP 70). GRP78 is a resident protein of the endoplasmic reticulum (ER) and may associate transiently with a variety of newly synthesized secretory and membrane proteins or permanently with mutant or defective proteins that are incorrectly folded, thus preventing their export from the ER lumen. GRP78 is a highly conserved protein that is essential for cell viability. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C terminus of GRP78 and other resident ER proteins including glucose regulated protein 94 (GRP 94) and protein disulfide isomerase (PDI). The presence of carboxy terminal KDEL appears to be necessary for retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.
Function : Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.
Subcellular Location : Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
DISEASE : Note=Autoantigen in rheumatoid arthritis.
Similarity : Belongs to the heat shock protein 70 family.
Database links :
Entrez Gene: 3309 Human
Entrez Gene: 14828 Mouse
Entrez Gene: 25617 Rat
Omim: 138120 Human
SwissProt: P11021 Human
SwissProt: P20029 Mouse
SwissProt: P06761 Rat
Unigene: 605502 Human
Unigene: 743241 Human
Unigene: 330160 Mouse
Unigene: 470180 Mouse
Unigene: 11088 Rat
葡萄糖调节蛋白78是热休克蛋白70家族的成员之一又称:热休克蛋白70蛋白5,作为一种分子伴侣在蛋白质的折叠和转运过程及内质网应激反应中发挥重要作用.它在多种肿瘤组织中呈高表达.