葡萄糖转运蛋白3抗体英文名称 Anti-GLUT3
中文名称 葡萄糖转运蛋白3抗体
别 名 brain; facilitated glucose transporter member 3; Glucose Transporter GLUT3 ; FLJ90380; Glucose Transporter Type 3; Glucose transporter type 3 brain; GLUT 3; GLUT-3; GLUT3; GTR3_HUMAN; SLC2A3; Solute Carrier Family 2 (Facilitated Glucose Transporter) Member 3; Solute carrier family 2.
浓 度 1mg/1ml
规 格 0.1ml/100μg 0.2ml/200μg
纯化的抗体可通过不同的途径获取,有些葡萄糖转运蛋白3抗体可通过下述方法制备或从商家购买。从商家购买的抗体,通常附有正确的储存方法。
1)工作液应在4℃下融化并存放,可能稳定达数月。
2)如果没有特殊原因而避免使用叠氮钠,亦可加入叠氮钠,浓度为0.02%。将纯化的抗体样本分装成合适的体积,于-20℃保存。
3)纯化的抗体溶液应以较高的浓度(如lmg/m1)在中性pH下保存。:常用的抗体储存浓度高达l0mg/ml。较低浓度的抗体冻存前应浓缩。所有标准的浓缩方法(如超滤法),皆可使用。还有一个简单的方法是用蛋白A或蛋白G亲和柱来浓缩溶液。如果纯化的抗体不是用于标记,可将它们以较低浓度储存于加有1%BSA的溶液中。
4)经纯化制备的抗体在常用的缓冲液中是稳定的。其DH应保持在中性左右。如果pH在7-8之间,即使保存多年,对抗体也无损害。多数情况下,盐浓度适于保持在0-150mmol/L之间,但在长期存放的抗体中,盐溶液浓度高达500mmol/L时,对葡萄糖转运蛋白3抗体能有损害。如果没有其他说明.律议用PBS或50mmol/LTris(DH8.0)溶液长期存放抗体。
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, Guinea Pig
产品类型 一抗
研究领域 免疫学
蛋白分子量 predicted molecular weight: 54kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human GLUT3
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
葡萄糖转运蛋白3抗体产品介绍 may act as a glucose transporter in neurons; may mediate increasd glucose uptake in response to neuronal injury. Glucose is fundamental to the metabolism of mammalian cells. Several glucose transporter protein (Glut) isoforms have been identified and shown to function in response to insulin and IGF1 induced signaling. GLUT3 is detectable in a few normal cell type spermatids in testis with active spermatogenesis, placental trophoblast membranes, and neurons in brain. GLUT3 staining is also detectable in human cancers including those of the ovary, lung, and testis. Alternative names: FLJ90380; Glucose Transporter Type 3; Glucose transporter type 3 brain; GLUT 3; GLUT3; SLC2A3; Solute Carrier Family 2 (Facilitated Glucose Transporter) Member 3.
Function : Facilitative glucose transporter. Probably a neuronal glucose transporter.
Subcellular Location : Cell membrane; Multi-pass membrane protein. Melanosome.
Tissue Specificity : Highly expressed in brain. Expressed in many tissues.
Similarity : Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
交换和转运(Trafficking and Transport)
GLUT-3属于溶质运载蛋白家族成员(solute carrier family),主要功能是转载葡萄糖进入上皮细胞。
目前主要用于糖尿病肾病和视网膜病变的研究,也是肾小球系膜细胞上的主要葡萄糖转运体。GLUT3的功能状态直接影响系膜细胞的糖代谢及功能变化。
近期,研究人员也用来区别一些良、恶性肿瘤的鉴别。