胃泌素抑制肽抗体

胃泌素抑制肽抗体英文名称  Anti-GIP 

中文名称  胃泌素抑制肽抗体 

别    名  Gastric Inhibitory polypeptide; Gastric inhibitory polypeptide precursor; GIP; Glucose dependent insulinotropic polypeptide. 

浓    度  1mg/1ml 

规 格  0.1ml/100μg  0.2ml/200μg  1ml/1mg   

纯化的抗体可通过不同的途径获取，有些胃泌素抑制肽抗体可通过下述方法制备或从商家购买。从商家购买的抗体，通常附有正确的储存方法。

1）工作液应在4℃下融化并存放，可能稳定达数月。

2）如果没有特殊原因而避免使用叠氮钠，亦可加入叠氮钠，浓度为0．02％。将纯化的抗体样本分装成合适的体积，于-20℃保存。

3）纯化的抗体溶液应以较高的浓度(如lmg／m1)在中性pH下保存。：常用的抗体储存浓度高达l0mg/ml。较低浓度的抗体冻存前应浓缩。所有标准的浓缩方法(如超滤法)，皆可使用。还有一个简单的方法是用蛋白A或蛋白G亲和柱来浓缩溶液。如果纯化的抗体不是用于标记，可将它们以较低浓度储存于加有1％BSA的溶液中。

4）经纯化制备的抗体在常用的缓冲液中是稳定的。其DH应保持在中性左右。如果pH在7-8之间，即使保存多年，对抗体也无损害。多数情况下，盐浓度适于保持在0-150mmol/L之间，但在长期存放的抗体中，盐溶液浓度高达500mmol/L时，对胃泌素抑制肽抗体能有损害。如果没有其他说明．律议用PBS或50mmol/LTris(DH8．0)溶液长期存放抗体。  

抗体来源  Rabbit  

克隆类型  polyclonal 

交叉反应  Human, Mouse, Rat, Pig, Cow

产品类型  一抗    

研究领域  信号转导 生长因子和激素 转录调节因子 糖尿病  

蛋白分子量  predicted molecular weight: 16kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated synthetic peptide derived from human GIP C-terminus 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide 

产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

胃泌素抑制肽抗体产品介绍 GIP (Gastric Inhibitory polypeptide ) belongs to the glucagon superfamily. The encoded protein is important in maintaining glucose homeostasis as it is a potent stimulator of insulin secretion from pancreatic beta-cells following food ingestion and nutrient absorption. This gene stimulates insulin secretion via its G protein-coupled receptor activation of adenylyl cyclase and other signal transduction pathways. It is a relatively poor inhibitor of gastric acid secretion. Mature GIP is a highly conserved 42 amino acid polypeptide belonging to the glucagon family. GIP is highly expressed as a pre-pro-protein in the duodenum and the jejunum. The mature secreted polypeptide acts as a potent stimulator of insulin secretion and a poor inhibitor of gastric acid secretion.

Function : Potent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion.

Subcellular Location : Secreted.

Similarity : Belongs to the glucagon family.

胃泌素抑制肽（GIP）是胃肠道的一种由42个氨基酸组成的多肽激素，又称为葡萄糖依赖性促胰岛素多肽（glucose-dependent insulinotropic peptide，GIP）他强烈抑制胃分泌和运动，也可调节胰岛素的释放。GIP属于Glucagon家族成员。