G蛋白偶联受体70抗体英文名称 Anti-GPR70/T1R1
中文名称 G蛋白偶联受体70抗体
别 名 GPCR TAS1R1; G protein coupled receptor 70; G-protein coupled receptor 70; gm 148; gm148; GPR 70; GPR70; Krueppel related zinc finger protein 3 (HKR3 protein); Sweet taste receptor T1r; T1R1; TAS1 R1; TAS1R1; Taste receptor type 1 member 1; TR 1; TR1; TS1R1_HUMAN.
浓 度 1mg/1ml
规 格 0.2ml/200μg
纯化的抗体可通过不同的途径获取,有些G蛋白偶联受体70抗体可通过下述方法制备或从商家购买。从商家购买的抗体,通常附有正确的储存方法。
1)工作液应在4℃下融化并存放,可能稳定达数月。
2)如果没有特殊原因而避免使用叠氮钠,亦可加入叠氮钠,浓度为0.02%。将纯化的抗体样本分装成合适的体积,于-20℃保存。
3)纯化的抗体溶液应以较高的浓度(如lmg/m1)在中性pH下保存。:常用的抗体储存浓度高达l0mg/ml。较低浓度的抗体冻存前应浓缩。所有标准的浓缩方法(如超滤法),皆可使用。还有一个简单的方法是用蛋白A或蛋白G亲和柱来浓缩溶液。如果纯化的抗体不是用于标记,可将它们以较低浓度储存于加有1%BSA的溶液中。
4)经纯化制备的抗体在常用的缓冲液中是稳定的。其DH应保持在中性左右。如果pH在7-8之间,即使保存多年,对抗体也无损害。多数情况下,盐浓度适于保持在0-150mmol/L之间,但在长期存放的抗体中,盐溶液浓度高达500mmol/L时,对G蛋白偶联受体70抗体能有损害。如果没有其他说明.律议用PBS或50mmol/LTris(DH8.0)溶液长期存放抗体。
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Horse
产品类型 一抗
研究领域 神经生物学 信号转导 G蛋白偶联受体
蛋白分子量 predicted molecular weight: 90kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human GPR70/T1R1
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
G蛋白偶联受体70抗体产品介绍 GPCR TAS1R1; G protein coupled receptor 70; G-protein coupled receptor 70; gm 148; gm148; GPR 70; GPR70; Krueppel related zinc finger protein 3 (HKR3 protein); Sweet taste receptor T1r; T1R1; TAS1 R1; TAS1R1; Taste receptor type 1 member 1; TR 1; TR1; TS1R1_HUMAN.
Function : Putative taste receptor. TAS1R1/TAS1R3 responds to the umami taste stimulus (the taste of monosodium glutamate). Sequence differences within and between species can significantly influence the selectivity and specificity of taste responses.
Subunit : Forms heterodimers with TAS1R3.
Subcellular Location : Cell membrane; Multi-pass membrane protein.
Similarity : Belongs to the G-protein coupled receptor 3 family. TAS1R subfamily.