鸟嘌呤核苷酸结合蛋白X抗体

鸟嘌呤核苷酸结合蛋白X抗体英文名称  Anti-GNRPX/PLEKHJ1 

中文名称  鸟嘌呤核苷酸结合蛋白X抗体 

别    名  Guanine nucleotide releasing protein x; Guanine nucleotide-releasing protein x; Likely ortholog of mouse guanine nucleotide releasing protein x; PH domain containing family J member 1; PH domain-containing family J member 1; PKHJ1_HUMAN; Pleckstrin homology domain containing family J member 1; Pleckstrin homology domain-containing family J member 1; PLEKHJ 1; PLEKHJ1; 9530063M10Rik; FLJ10297. 

浓    度  1mg/1ml 

规 格  0.2ml/200μg     

纯化的抗体可通过不同的途径获取，有些鸟嘌呤核苷酸结合蛋白X抗体可通过下述方法制备或从商家购买。从商家购买的抗体，通常附有正确的储存方法。

1）工作液应在4℃下融化并存放，可能稳定达数月。

2）如果没有特殊原因而避免使用叠氮钠，亦可加入叠氮钠，浓度为0．02％。将纯化的抗体样本分装成合适的体积，于-20℃保存。

3）纯化的抗体溶液应以较高的浓度(如lmg／m1)在中性pH下保存。：常用的抗体储存浓度高达l0mg/ml。较低浓度的抗体冻存前应浓缩。所有标准的浓缩方法(如超滤法)，皆可使用。还有一个简单的方法是用蛋白A或蛋白G亲和柱来浓缩溶液。如果纯化的抗体不是用于标记，可将它们以较低浓度储存于加有1％BSA的溶液中。

4）经纯化制备的抗体在常用的缓冲液中是稳定的。其DH应保持在中性左右。如果pH在7-8之间，即使保存多年，对抗体也无损害。多数情况下，盐浓度适于保持在0-150mmol/L之间，但在长期存放的抗体中，盐溶液浓度高达500mmol/L时，对鸟嘌呤核苷酸结合蛋白X抗体能有损害。如果没有其他说明．律议用PBS或50mmol/LTris(DH8．0)溶液长期存放抗体。  

抗体来源  Rabbit  

克隆类型  polyclonal 

交叉反应  Human, Mouse, Rat, Pig, Cow, Sheep

产品类型  一抗    

研究领域  细胞生物 免疫学 信号转导 G蛋白信号  

蛋白分子量  predicted molecular weight: 18kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated synthetic peptide derived from human GNRPX/PLEKHJ1 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 

产品应用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20°C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4°C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

鸟嘌呤核苷酸结合蛋白X抗体产品介绍 PLEKHJ1 is a 149 amino acid phosphoprotein that contains one PH (pleckstrin homology) domain and is expressed in testis and liver. The gene that encodes PLEKHJ1 maps to human chromosome 19, which consists of approximately 63 million bases and makes up over 2% of human genomic DNA. Chromosome 19 contains the greatest gene density of the human chromosomes and is the genetic home for a number of immunoglobulin superfamily members, including killer cell and leukocyte Ig-like receptors, ICAMs, the CEACAM and PSG families, and Fc?receptors. Key genes for eye color and hair color also map to chromosome 19. Peutz-Jeghers syndrome, spinocerebellar ataxia type 6, the stroke disorder CADASIL, hypercholesterolemia and insulin-dependent diabetes are also linked to chromosome 19.

Tissue Specificity : Expressed in testis and liver.

Similarity : Contains 1 PH domain. 

Database links : UniProtKB/Swiss-Prot: Q9NW61.1