4)经纯化制备的抗体在常用的缓冲液中是稳定的。其DH应保持在中性左右。如果pH在7-8之间,即使保存多年,对抗体也无损害。多数情况下,盐浓度适于保持在0-150mmol/L之间,但在长期存放的抗体中,盐溶液浓度高达500mmol/L时,对
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep
产品类型 一抗
研究领域 肿瘤 细胞生物 免疫学
蛋白分子量 predicted molecular weight: 27kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human FGF8 C-terminus
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
成纤维细胞生长因子8抗体产品介绍 Keratinocyte growth factor precursor (KGF) (Fibroblast growth factor 8; FGF-8; HBGF-8). Stimulates growth of the cells in an autocrine manner. Mediates hormonal action on the growth of cancer cells. Cooperates with Wnt-1 in mouse mammary tumor virus-induced murine mammary tumorigenesis. [Subcellular location] Secreted. [Tissue specificity] Absent in normal mammary glands and detected only in adult testis and ovary and in midgestational embryos. [Induction] By androgens. Belongs to the heparin-binding growth factors family.
Function : Stimulates growth of the cells in an autocrine manner. Mediates hormonal action on the growth of cancer cells.
Subunit : Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors.
Subcellular Location : Secreted.
DISEASE : Defects in FGF8 are the cause of Kallmann syndrome type 6 (KAL6) [MIM:612702]. Kallmann syndrome is a disorder that associates hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. In some patients other developmental anomalies can be present, which include renal agenesis, cleft lip and/or palate, selective tooth agenesis, and bimanual synkinesis. In some cases anosmia may be absent or inconspicuous.
Similarity : Belongs to the heparin-binding growth factors family.
Database links :
UniProtKB/Swiss-Prot: P55075.1
Entrez Gene: 2253 Human
Omim: 600483 Human
SwissProt: P55075 Human
Unigene: 57710 Human
有研究者认为:纤维母细胞生长因子8 FGF8 对癌症具有一定的抑制作用。
早期研究FGF8,认为是自分泌生长因子,它在前列腺癌、乳癌和卵巢癌中常常表现过量表达 (over-expressed),并与前列腺癌的格里森分数 (Gleason score) 相关联。经过各种细胞和动物模型的实验均支持FGF8在抑制肿瘤形成和肿瘤细胞成活方面的作用。
此外,还有学者认为:FGF8 还在实验中显示出,他有改善因胶原蛋白引发的关节炎性的改变,如:风湿性关节炎和类风湿性关节炎及退行性骨性关节病。