4)经纯化制备的抗体在常用的缓冲液中是稳定的。其DH应保持在中性左右。如果pH在7-8之间,即使保存多年,对抗体也无损害。多数情况下,盐浓度适于保持在0-150mmol/L之间,但在长期存放的抗体中,盐溶液浓度高达500mmol/L时,对
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit, Sheep
产品类型 一抗
研究领域 信号转导 细胞膜受体 t-淋巴细胞
蛋白分子量 predicted molecular weight: 51kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human FKBP51
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 癈 for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20癈. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 癈.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
类固醇受体复合物蛋白FKBP5抗体产品介绍 The immunophilins are a highly conserved family of cis-trans peptidyl-prolyl isomerases that bind to and mediate the effects of immunosuppressive drugs, such as cyclosporin, FK506 and rapamycin (1). Several related immunophilins, FKBP12, FKBP51 and FKBP52, are characterized as cytosolic FK506-binding proteins, and following ligand binding, they functionally inhibit the phosphatase activity of calcineurin (2,3). The ubiquitously expressed FKBP12 also associates with the cytoplasmic domain of the TGF?type I receptor, where it stabilizes the inactive conformation of the receptor and blocks the activation of the TGF?pathway (4). FKBP51 and FKBP52 are two highly related proteins (5,6). FKBP51 is predominantly expressed in T cells and is induced by glucocorticoids (5). FKBP51 mediates the effects of FK506 and rapamycin by inhibiting intracellular calcineurin activity, and by blocking T-cell activation and proliferation (7). FKBP52, known also as FKBP-59 or heat shock protein 56, is expressed in a variety of tissues and can also associate with the heat shock protein (hsp90) in mature steroid receptor complexes (6,8).
Function : Interacts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP.
Subcellular Location : Cytoplasm. Nucleus.
Tissue Specificity : Widely expressed, enriched in testis compared to other tissues.
Post-translational modifications : Phosphorylated upon DNA damage, probably by ATM or ATR.
Similarity : Contains 2 PPIase FKBP-type domains. Contains 3 TPR repeats.
Database links : UniProtKB/Swiss-Prot: Q13451.2