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脂肪堆积和肥胖相关蛋白抗体

供货周期：一周

品牌：

规格： 0.2ml/200μg

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CAS号：

报价： ¥1

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产品介绍

脂肪堆积和肥胖相关蛋白抗体英文名称 Anti-FTO/Protein fatso

中文名称脂肪堆积和肥胖相关蛋白抗体

别名 Alpha-ketoglutarate-dependent dioxygenase FTO; Fat mass and obesity-associated protein; Fto; FTO_HUMAN; Protein fatso.

浓度 1mg/1ml

规格 0.2ml/200μg

纯化的抗体可通过不同的途径获取，有些脂肪堆积和肥胖相关蛋白抗体可通过下述方法制备或从商家购买。从商家购买的抗体，通常附有正确的储存方法。

1）工作液应在4℃下融化并存放，可能稳定达数月。

2）如果没有特殊原因而避免使用叠氮钠，亦可加入叠氮钠，浓度为0．02％。将纯化的抗体样本分装成合适的体积，于-20℃保存。

3）纯化的抗体溶液应以较高的浓度(如lmg／m1)在中性pH下保存。：常用的抗体储存浓度高达l0mg/ml。较低浓度的抗体冻存前应浓缩。所有标准的浓缩方法(如超滤法)，皆可使用。还有一个简单的方法是用蛋白A或蛋白G亲和柱来浓缩溶液。如果纯化的抗体不是用于标记，可将它们以较低浓度储存于加有1％BSA的溶液中。

4）经纯化制备的抗体在常用的缓冲液中是稳定的。其DH应保持在中性左右。如果pH在7-8之间，即使保存多年，对抗体也无损害。多数情况下，盐浓度适于保持在0-150mmol/L之间，但在长期存放的抗体中，盐溶液浓度高达500mmol/L时，对脂肪堆积和肥胖相关蛋白抗体可能有损害。如果没有其他说明．律议用PBS或50mmol/LTris(DH8．0)溶液长期存放抗体。

抗体来源 Rabbit

克隆类型 polyclonal

交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep

产品类型一抗

研究领域心血管细胞生物免疫学神经生物学糖尿病内分泌病

蛋白分子量 predicted molecular weight: 58kDa

性状 Lyophilized or Liquid

免疫原 KLH conjugated synthetic peptide derived from human Protein fatso

亚型 IgG

纯化方法 affinity purified by Protein A

储存液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

脂肪堆积和肥胖相关蛋白抗体产品介绍 Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation.

Tissue specificity: Ubiquitously expressed, with relatively high expression in adrenal glands and brain; especially in hypothalamus and pituitary.

Function : Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation.

Subunit : Monomer. May also exist as homodimer.

Subcellular Location : Nucleus.

Tissue Specificity : Ubiquitously expressed, with relatively high expression in adrenal glands and brain; especially in hypothalamus and pituitary.

DISEASE : Defects in FTO are the cause of growth retardation developmental delay coarse facies and early death (GDFD) [MIM:612938]. A severe polymalformation syndrome characterized by postnatal growth retardation, microcephaly, severe psychomotor delay, functional brain deficits and characteristic facial dysmorphism. In some patients, structural brain malformations, cardiac defects, genital anomalies, and cleft palate are observed. Early death occurs by the age of 3 years.

Similarity : Belongs to the fto family.

Database links : UniProtKB/Swiss-Prot: Q9C0B1.3

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2013-03-11

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脂肪堆积和肥胖相关蛋白抗体由上海基免实业有限公司为您提供，货号，规格：0.2ml/200μg ，CAS号：，如您想了解更多关于脂肪堆积和肥胖相关蛋白抗体价格、脂肪堆积和肥胖相关蛋白抗体结构式、批发、用途等信息，欢迎咨询。除供应脂肪堆积和肥胖相关蛋白抗体外，还可为您提供赭曲霉毒素A(OTA)检测试剂盒、植物N-甲基腐胺氧化酶(MPO)检测试剂盒、呕吐毒素(vomitoxin)检测试剂盒等试剂，公司有专业的客户服务团队，是您值得信赖的合作伙伴，上海基免客户服务电话，售前、售后均可联系。

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