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着丝粒蛋白K

供货周期：一周

品牌：

规格： 0.2ml/200μg

货号：

CAS号：

报价： ¥1

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产品介绍

着丝粒蛋白K英文名称 Anti-FKSG14/Centromere protein K

中文名称着丝粒蛋白K

别名 AF5 alpha; AF5alpha; B130045K24Rik; BB232189; C530004N04Rik; CENP K; CENPK; Centromere protein K; Centromeric protein K; FKSG 14; ICEN 37; ICEN37; Interphase centromere complex protein 37; Leucine zipper protein FKSG14; OTTHUMP00000161888; P33; Protein AF 5alpha; Protein AF5alpha; Solt; Solzt; SoxLZ/Sox6 binding protein Solt; SoxLZ/Sox6 leucine zipper binding protein; CENPK_HUMAN.

浓度 1mg/1ml

规格 0.2ml/200μg

纯化的抗体可通过不同的途径获取，有些着丝粒蛋白K可通过下述方法制备或从商家购买。从商家购买的抗体，通常附有正确的储存方法。

1）工作液应在4℃下融化并存放，可能稳定达数月。

2）如果没有特殊原因而避免使用叠氮钠，亦可加入叠氮钠，浓度为0．02％。将纯化的抗体样本分装成合适的体积，于-20℃保存。

3）纯化的抗体溶液应以较高的浓度(如lmg／m1)在中性pH下保存。：常用的抗体储存浓度高达l0mg/ml。较低浓度的抗体冻存前应浓缩。所有标准的浓缩方法(如超滤法)，皆可使用。还有一个简单的方法是用蛋白A或蛋白G亲和柱来浓缩溶液。如果纯化的抗体不是用于标记，可将它们以较低浓度储存于加有1％BSA的溶液中。

4）经纯化制备的抗体在常用的缓冲液中是稳定的。其DH应保持在中性左右。如果pH在7-8之间，即使保存多年，对抗体也无损害。多数情况下，盐浓度适于保持在0-150mmol/L之间，但在长期存放的抗体中，盐溶液浓度高达500mmol/L时，对着丝粒蛋白K可能有损害。如果没有其他说明．律议用PBS或50mmol/LTris(DH8．0)溶液长期存放抗体。

抗体来源 Rabbit

克隆类型 polyclonal

交叉反应 Human, Mouse, Rat, Pig, Cow, Horse, Sheep

产品类型一抗

研究领域细胞生物免疫学表观遗传学

蛋白分子量 predicted molecular weight: 32kDa

性状 Lyophilized or Liquid

免疫原 KLH conjugated synthetic peptide derived from human FKSG14/Centromere protein K

亚型 IgG

纯化方法 affinity purified by Protein A

储存液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200

（石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

着丝粒蛋白K产品介绍 FKSG14, also known as CENPK (centromere protein K) is a component of the CENPA-CAD (nucleosome distal) complex. It may be involved in incorporation of CENPA into centromeres and is required for proper kinetochore function, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with CASC5/KNL1 to recruit the NDC80 complex to the outer kinetochore. FKSG14 constitutively localized to centromeres throughout the cell cycle. There are 3 isoforms produced by alternative splicing.

Function : Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with CASC5/KNL1 to recruit the NDC80 complex to the outer kinetochore.

Subunit : Component of the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. The CENPA-CAD complex interacts with the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and MLF1IP/CENPU. Interacts directly with CENPH.

Subcellular Location : Nuclear. Centromere. Kinetochore. Localizes exclusively in the centromeres. Note=Localizes exclusively in the centromeres. The CENPA-CAD complex is probably recruited on centromeres by the CENPA-NAC complex.

Tissue Specificity : Detected in several fetal organs with highest levels in fetal liver. In adults, it is weakly expressed in lung and placenta.

DISEASE : Note=Chromosomal aberrations involving CENPK are a cause of acute leukemias. Translocation t(5;11)(q12;q23) with MLL.

Similarity : Belongs to the CENPK family.

Database links : UniProtKB/Swiss-Prot: Q9BS16.1

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上海基免实业有限公司

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2013-03-11

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着丝粒蛋白K由上海基免实业有限公司为您提供，货号，规格：0.2ml/200μg ，CAS号：，如您想了解更多关于着丝粒蛋白K价格、着丝粒蛋白K结构式、批发、用途等信息，欢迎咨询。除供应着丝粒蛋白K外，还可为您提供虾淀粉酶α(Amyα)检测试剂盒、植物S-鸟氨酸转氨酶(OTC)检测试剂盒、赭曲霉毒素A(OTA)检测试剂盒等试剂，公司有专业的客户服务团队，是您值得信赖的合作伙伴，上海基免客户服务电话，售前、售后均可联系。

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