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当前位置: 上海基免 > 抗体/抗原 > 磷酸化DNA损伤修复基因XRCC9抗体

磷酸化DNA损伤修复基因XRCC9抗体

供货周期: 一周
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规格: 0.2ml/200μg
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CAS号:
报价: ¥1
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产品介绍
磷酸化DNA损伤修复基因XRCC9抗体英文名称  Anti-phospho-FANCG (Ser383) 
中文名称  磷酸化DNA损伤修复基因XRCC9抗体 
别    名  FANCG (phospho S383); p-FANCG (phospho S383); DNA repair protein XRCC9; DNA-repair protein XRCC9; FAG; Fanconi anaemia complementation group G; Protein FACG; X ray repair, complementing defective, in Chinese hamster cells 9; XRCC9. 
浓    度  1mg/1ml 
规 格  0.1ml/100μg     
纯化的抗体可通过不同的途径获取,有些磷酸化DNA损伤修复基因XRCC9抗体可通过下述方法制备或从商家购买。从商家购买的抗体,通常附有正确的储存方法。
1)工作液应在4℃下融化并存放,可能稳定达数月。
2)如果没有特殊原因而避免使用叠氮钠,亦可加入叠氮钠,浓度为0.02%。将纯化的抗体样本分装成合适的体积,于-20℃保存。
3)纯化的抗体溶液应以较高的浓度(如lmg/m1)在中性pH下保存。:常用的抗体储存浓度高达l0mg/ml。较低浓度的抗体冻存前应浓缩。所有标准的浓缩方法(如超滤法),皆可使用。还有一个简单的方法是用蛋白A或蛋白G亲和柱来浓缩溶液。如果纯化的抗体不是用于标记,可将它们以较低浓度储存于加有1%BSA的溶液中。
4)经纯化制备的抗体在常用的缓冲液中是稳定的。其DH应保持在中性左右。如果pH在7-8之间,即使保存多年,对抗体也无损害。多数情况下,盐浓度适于保持在0-150mmol/L之间,但在长期存放的抗体中,盐溶液浓度高达500mmol/L时,对磷酸化DNA损伤修复基因XRCC9抗体可能有损害。如果没有其他说明.律议用PBS或50mmol/LTris(DH8.0)溶液长期存放抗体。     
抗体来源  Rabbit  
克隆类型  polyclonal 
交叉反应  Human, Mouse, Rat
产品类型  一抗  磷酸化抗体   
研究领域  细胞生物 免疫学 染色质和核信号  
蛋白分子量  predicted molecular weight: 69kDa 
性    状  Lyophilized or Liquid 
免 疫 原  KLH conjugated Synthesised phosphopeptide derived from human FANCG around the phosphorylation site of Ser383 
亚    型  IgG 
纯化方法  affinity purified by Protein A 
储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide 
产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500 
(石蜡切片需做抗原修复) 
 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user.  
保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 
Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
磷酸化DNA损伤修复基因XRCC9抗体产品介绍 FANCG, involved in Fanconi anemia, confers resistance to both hygromycin and mitomycin C. FANCG contains a 5-prime GC-rich untranslated region characteristic of housekeeping genes. The putative 622-amino acid protein has a leucine-zipper motif at its N-terminus. Fanconi anemia is an autosomal recessive disorder with diverse clinical symptoms, including developmental anomalies, bone marrow failure, and early occurrence of malignancies. A minimum of 8 FA genes have been identified.
Function : DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Candidate tumor suppressor gene. 
Subunit : Belongs to the multisubunit FA complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM. The complex is not found in FA patients. In complex with FANCF, FANCA and FANCL, but not with FANCC, nor FANCE, interacts with HES1; this interaction may be essential for the stability and nuclear localization of FA core complex proteins. The complex with FANCC and FANCG may also include EIF2AK2 and HSP70. When phosphorylated at Ser-7, forms a complex with BRCA2, FANCD2 and XRCC3. 
Subcellular Location : Nucleus. Cytoplasm. Note=The major form is nuclear. The minor form is cytoplasmic. 
Tissue Specificity : Highly expressed in testis and thymus. Found in lymphoblasts. 
DISEASE : Defects in FANCG are a cause of Fanconi anemia complementation group G (FANCG) [MIM:614082]. A disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair. 
Similarity : Contains 4 TPR repeats. 
Database links : UniProtKB/Swiss-Prot: O15287.1
 

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2013-03-11

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