4)经纯化制备的抗体在常用的缓冲液中是稳定的。其DH应保持在中性左右。如果pH在7-8之间,即使保存多年,对抗体也无损害。多数情况下,盐浓度适于保持在0-150mmol/L之间,但在长期存放的抗体中,盐溶液浓度高达500mmol/L时,对
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse
产品类型 一抗
研究领域 肿瘤 细胞生物 免疫学 神经生物学
蛋白分子量 predicted molecular weight: 78kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human Fbxw7/CDC4
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Fbxw7蛋白抗体产品介绍 The F-box protein family is characterized by an approximately 40 amino acid motif known as the F-box. F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. One family member, Cdc4, also known as AGO, FBW7, FBXW7, FBX30, SEL10, and FLJ11071, maps to human chromosome 4q31.3. Alternative splicing of this gene generates four transcript variants. In addition to an F-box, Cdc4 contains seven tandem WD40 repeats. Cdc4 binds directly to cyclin E and targets cyclin E for ubiquitin-mediated degradation. Mutations of the Cdc4 gene are detected in ovarian and breast cancer cell lines, suggesting that the gene may be involved in the pathogenesis of human cancers.
Function : Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins. Involved in the degradation of cyclin-E, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1.
Subunit : Component of the SCF(FBXW7) complex consisting of CUL1, RBX1, SKP1 and FBXW7. Interacts with PSEN1, cyclin E, NOTCH1 NICD, NOTCH4 NICD and SKP1. Interacts with MYC (when phosphorylated). Isoform 1 interacts with USP28, leading to counteract ubiquitination of MYC. Isoform 4 interacts (via WD repeats) with SV40 large T antigen (via CPD region). Forms a trimeric complex with NOTCH1 and SGK1.
Subcellular Location : Isoform 1: Nucleus, nucleoplasm. Isoform 2: Cytoplasm. Isoform 4: Nucleus, nucleolus. Nucleus.
Tissue Specificity : Isoform 1 is widely expressed. Isoform 4 is expressed in brain.
Post-translational modifications : Phosphorylated upon DNA damage, probably by ATM or ATR.
Similarity : Contains 1 F-box domain.
Contains 7 WD repeats.
Database links : UniProtKB/Swiss-Prot: Q969H0.1