效率的因素主要有5个方面：①抗体对抗原的亲合性；②抗体对抗原的特异性；③抗原表位结构在该技术中是如何被改变的；④抗体与抗原结合的易接近性；⑤二级试剂的质量和类型
英文名称 Anti-EphA5/Eph receptor A5
中文名称 酪氨酸蛋白激酶A5受体抗体
别 名 bsk; CEK 7; Eck like sequence 1; EHK 1; Els 1; Eph homology kinase 1; EphA5; Ephrin type A receptor 5; HEK 7; Receptor protein tyrosine kinase HEK 7; Rek 7; TYRO 4; Tyrosine protein kinase receptor EHK 1.
酪氨酸蛋白激酶A5受体抗体浓 度 1mg/1ml
规 格 0.1ml/100μg 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Pig, Cow, Rabbit, Guinea Pig
产品类型 一抗
研究领域 心血管 细胞生物 发育生物学 神经生物学 信号转导 激酶和磷酸酶
蛋白分子量 predicted molecular weight: 114kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human EphA5/Eph receptor A5
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 EphA5, also known as Ehk1, Bsk, Cek7, Hek7, and Rek7, is a member of the Eph receptor family, which binds members of the Ephrin ligand family. Two classes of receptors exist, designated A and B, that have an extracellular domain made up of a globular domain, a cysteine-rich domain, and two fibronectin type III domains, followed by the transmembrane region and cytoplasmic region. The cytoplasmic region is a juxtamembrane region with two tyrosines, the major autophosphorylation sites, along with a kinase domain, and a conserved sterile alpha motif (SAM) in the C-terminus, the latter including one conserved tyrosine.
Subunit : Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses.
Subcellular Location : Cell membrane; Single-pass type I membrane protein. Cell projection, axon. Cell projection, dendrite.
Tissue Specificity : Almost exclusively expressed in the nervous system in cortical neurons, cerebellar Purkinje cells and pyramidal neurons within the cortex and hippocampus. Display an increasing gradient of expression from the forebrain to hindbrain and spinal cord.
Similarity : Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 1 Eph LBD (Eph ligand-binding) domain.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain.
半个多世纪以来，抗体一直作为常用的研究工具，因此对其使用和储存已有丰富的经验。这些经验让人们知道如何对抗体进行操作，由于抗体的稳定性好，使用并不困难。抗体较其他蛋白不易变性，易于存放、纯化和标记。蛋白G和蛋白A等抗体结合蛋白的发现使得这项工作更具可行性。
选择合适的抗体对免疫化学技术的成功极为重要。没有任何一种来源的抗体能够满足本书所述的各种方法的要求。为了研究某一重要的抗原，应选用具有不同特性的多种抗体。在没有一套良好特性的抗体可用于研究机体中每一种蛋白之前，选择及制备适合各种技术要求的抗体非常必要。
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J12350 染色框同源物3(CBX3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Chromobox Homolog 3 (CBX3) 48T/96T Mus musculus (Mouse)
J12351 Cathelicidin抗菌肽(CAMP)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Cathelicidin Antimicrobial Peptide (CAMP) 48T/96T Homo sapiens (Human)
J12352 核仁磷酸蛋白(NPM)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Nucleophosmin (NPM) 48T/96T Mus musculus (Mouse)
J12353 2',5'-寡腺苷酸合成酶1(OAS1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for 2',5'-Oligoadenylate Synthetase 1 (OAS1) 48T/96T Mus musculus (Mouse)
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J12355 戊糖素(PTD)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Pentosidine (PTD) 48T/96T General
J12356 血管抑素(ANG)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Angiostatin (ANG) 48T/96T Mus musculus (Mouse)
J12357 密封蛋白(OCLN)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Occludin (OCLN) 48T/96T Homo sapiens (Human)
J12358 封闭蛋白1(CLDN1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Claudin 1 (CLDN1) 48T/96T Homo sapiens (Human)
J12359 周期素依赖性激酶抑制因子1A(CDKN1A)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) 48T/96T Mus musculus (Mouse)
J12360 封闭蛋白4(CLDN4)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Claudin 4 (CLDN4) 48T/96T Homo sapiens (Human)
J12361 成纤维细胞生长因子7(FGF7)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Fibroblast Growth Factor 7 (FGF7) 48T/96T Mus musculus (Mouse)
J12362 血管活性肠肽受体1(VIPR1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Vasoactive Intestinal Peptide Receptor 1 (VIPR1) 48T/96T Homo sapiens (Human)
J12363 失调蛋白1(ATXN1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Ataxin 1 (ATXN1) 48T/96T Homo sapiens (Human)
J12364 Ⅵ型胶原α1(COL6α1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Collagen Type VI Alpha 1 (COL6a1) 48T/96T Homo sapiens (Human)
J12365 中脑星形胶质细胞来源神经营养因子(MANF)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) 48T/96T Homo sapiens (Human)
J12366 酸性葡萄糖苷酶β(GβA)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Glucosidase Beta, Acid (GbA) 48T/96T Homo sapiens (Human)
J12367 Syncollin蛋白(SYCN)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Syncollin (SYCN) 48T/96T Homo sapiens (Human)
J12368 Thy1细胞表面抗原(Thy1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Thy1 Cell Surface Antigen (Thy1) 48T/96T Mus musculus (Mouse)
J12369 α1-酸性糖蛋白(α1AGP)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Alpha-1-Acid Glycoprotein (a1AGP) 48T/96T Sus scrofa; Porcine (Pig)
J12370 P-选择素糖蛋白配体1(PSGL1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for P-Selectin Glycoprotein Ligand 1 (PSGL1) 48T/96T Mus musculus (Mouse)
J12371 肿瘤坏死因子配体超家族成员7(TNFSF7)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tumor Necrosis Factor Ligand Superfamily, Member 7 (TNFSF7) 48T/96T Homo sapiens (Human)
J12372 成纤维细胞生长因子受体2(FGFR2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Fibroblast Growth Factor Receptor 2 (FGFR2) 48T/96T Homo sapiens (Human)
J12373 羧肽酶A2(CPA2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Carboxypeptidase A2, Pancreatic (CPA2) 48T/96T Rattus norvegicus (Rat)
J12374 羧肽酶A1(CPA1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Carboxypeptidase A1, Pancreatic (CPA1) 48T/96T Rattus norvegicus (Rat)
J12375 间皮素(MSLN)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Mesothelin (MSLN) 48T/96T Homo sapiens (Human)
J12376 V-Ets骨髓成红细胞增多症病毒E26癌基因同源物1(ETS1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1) 48T/96T Mus musculus (Mouse)
J12377 颗粒体蛋白(GRN)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Granulin (GRN) 48T/96T Homo sapiens (Human)
J12378 分泌型卷曲相关蛋白4(SFRP4)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Secreted Frizzled Related Protein 4 (SFRP4) 48T/96T Mus musculus (Mouse)
J12379 视锥蛋白样蛋白1(VSNL1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Visinin Like Protein 1 (VSNL1) 48T/96T Homo sapiens (Human)
J12380 丝甘蛋白聚糖(SRGN)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Serglycin (SRGN) 48T/96T Homo sapiens (Human)
J12381 Ⅷ型胶原α1(COL8α1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Collagen Type VIII Alpha 1 (COL8a1) 48T/96T Homo sapiens (Human)
J12382 甲状旁腺激素(PTH)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Parathyroid Hormone (PTH) 48T/96T Canis familiaris; Canine (Dog)
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J12389 Gremlin 1蛋白(GREM1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Gremlin 1 (GREM1) 48T/96T Homo sapiens (Human)
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J12391 骺蛋白聚糖(EPYC)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Epiphycan (EPYC) 48T/96T Homo sapiens (Human)
J12392 生长激素2(GH2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Growth Hormone 2 (GH2) 48T/96T Homo sapiens (Human)
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J12394 神经纤维网蛋白1(NRP1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Neuropilin 1 (NRP1) 48T/96T Rattus norvegicus (Rat)
J12395 线粒体解偶联蛋白2(UCP2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Uncoupling Protein 2, Mitochondrial (UCP2) 48T/96T Homo sapiens (Human)
J12396 保护素(CD59)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Protectin (CD59) 48T/96T Homo sapiens (Human)
J12397 吻素1(KISS1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Kisspeptin 1 (KISS1) 48T/96T Rattus norvegicus (Rat)
J12398 胰高血糖素样肽2(GLP2)酪氨酸蛋白激酶A5受体抗体检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Glucagon Like Peptide 2 (GLP2) 48T/96T Homo sapiens (Human)
J12399 Ⅰ型胶原α1(COL1α1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Collagen Type I Alpha 1 (COL1a1) 48T/96T Homo sapiens (Human)
J12400 Ⅺ型胶原α1(COL11α1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Collagen Type XI Alpha 1 (COL11a1) 48T/96T Homo sapiens (Human)
J12401 透明质酸结合蛋白2(HABP2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Hyaluronan Binding Protein 2 (HABP2) 48T/96T Homo sapiens (Human)
J12402 癌胚抗原相关细胞粘附分子7(CEACAM7)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Carcinoembryonic Antigen Related Cell Adhesion Molecule 7 (CEACAM7) 48T/96T Homo sapiens (Human)
更多酪氨酸蛋白激酶A5受体抗体价格、批发价、品牌、厂家及抗体详细说明书，都可以免费咨询及索取。
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