效率的因素主要有5个方面：①抗体对抗原的亲合性；②抗体对抗原的特异性；③抗原表位结构在该技术中是如何被改变的；④抗体与抗原结合的易接近性；⑤二级试剂的质量和类型
英文名称 Anti-EZH1
中文名称 组蛋白赖氨酸N-甲基EZH1抗体
别 名 Enhancer of zeste homolog 1(Drosophila); Enhancer of zeste homolog 1; ENX-2; ENX2; EZH1; EZH1: enhancer of zeste homolog 1 (Drosophila); EZH1_HUMAN; Histone-lysine N-methyltransferase EZH1; KIAA0388.
组蛋白赖氨酸N-甲基EZH1抗体浓 度 1mg/1ml
规 格 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Guinea Pig
产品类型 一抗
研究领域 肿瘤 染色质和核信号 肿瘤细胞生物标志物 表观遗传学
蛋白分子量 predicted molecular weight: 95kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human EZH1 (471-510aa)
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.
Sequence similarities
Belongs to the histone-lysine methyltransferase family. EZ subfamily. Contains 1 SET domain.
Function : Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.
Subunit : Component of the PRC2/EED-EZH1 complex, which includes EED, EZH1, SUZ12, RBBP4 and AEBP2. The PRC2/EED-EZH1 is less abundant than the PRC2/EED-EZH2 complex, has weak methyltransferase activity and compacts chromatin in the absence of the methyltransferase cofactor S-adenosyl-L-methionine (SAM).
Subcellular Location : Nucleus. Note=Colocalizes with trimethylated 'Lys-27' of histone H3.
Similarity : Belongs to the histone-lysine methyltransferase family. EZ subfamily.
Contains 1 CXC domain.
Contains 1 SET domain.
半个多世纪以来，抗体一直作为常用的研究工具，因此对其使用和储存已有丰富的经验。这些经验让人们知道如何对抗体进行操作，由于抗体的稳定性好，使用并不困难。抗体较其他蛋白不易变性，易于存放、纯化和标记。蛋白G和蛋白A等抗体结合蛋白的发现使得这项工作更具可行性。
选择合适的抗体对免疫化学技术的成功极为重要。没有任何一种来源的抗体能够满足本书所述的各种方法的要求。为了研究某一重要的抗原，应选用具有不同特性的多种抗体。在没有一套良好特性的抗体可用于研究机体中每一种蛋白之前，选择及制备适合各种技术要求的抗体非常必要。
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J11519 双调蛋白(AREG)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Amphiregulin (AREG) 48T/96T Sus scrofa; Porcine (Pig)
J11520 胰岛素样生长因子2(IGF2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Insulin Like Growth Factor 2 (IGF2) 48T/96T Bos taurus; Bovine (Cattle)
J11521 胰岛素样生长因子2(IGF2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Insulin Like Growth Factor 2 (IGF2) 48T/96T Capra hircus; Caprine (Goat)
J11522 胰岛素样生长因子2(IGF2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Insulin Like Growth Factor 2 (IGF2) 48T/96T Equus caballus; Equine (Horse)
J11523 胰岛素样生长因子2(IGF2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Insulin Like Growth Factor 2 (IGF2) 48T/96T Chicken (Gallus)
J11524 胰岛素样生长因子2(IGF2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Insulin Like Growth Factor 2 (IGF2) 48T/96T Cavia (Guinea pig )
J11525 胰岛素样生长因子结合蛋白1(IGFBP1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Insulin Like Growth Factor Binding Protein 1 (IGFBP1) 48T/96T Bos taurus; Bovine (Cattle)
J11526 胰岛素样生长因子结合蛋白1(IGFBP1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Insulin Like Growth Factor Binding Protein 1 (IGFBP1) 48T/96T Capra hircus; Caprine (Goat)
J11527 胰岛素样生长因子结合蛋白1(IGFBP1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Insulin Like Growth Factor Binding Protein 1 (IGFBP1) 48T/96T Danio rerio (Zebrafish)
J11528 内皮细胞TEK酪氨酸激酶(Tie2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for TEK Tyrosine Kinase, Endothelial (Tie2) 48T/96T Bos taurus; Bovine (Cattle)
J11529 内皮细胞TEK酪氨酸激酶(Tie2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for TEK Tyrosine Kinase, Endothelial (Tie2) 48T/96T Danio rerio (Zebrafish)
J11530 内皮细胞TEK酪氨酸激酶(Tie2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for TEK Tyrosine Kinase, Endothelial (Tie2) 48T/96T Mus musculus (Mouse)
J11531 组织金属蛋白酶抑制因子2(TIMP2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) 48T/96T Bos taurus; Bovine (Cattle)
J11532 组织金属蛋白酶抑制因子2(TIMP2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) 48T/96T Canis familiaris; Canine (Dog)
J11533 组织金属蛋白酶抑制因子2(TIMP2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) 48T/96T Equus caballus; Equine (Horse)
J11534 组织金属蛋白酶抑制因子2(TIMP2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) 48T/96T Danio rerio (Zebrafish)
J11535 组织金属蛋白酶抑制因子2(TIMP2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) 48T/96T Chicken (Gallus)
J11536 组织金属蛋白酶抑制因子2(TIMP2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) 48T/96T Cavia (Guinea pig )
J11537 组织金属蛋白酶抑制因子2(TIMP2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) 48T/96T Sus scrofa; Porcine (Pig)
J11538 组织金属蛋白酶抑制因子2(TIMP2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) 48T/96T Oryctolagus cuniculus (Rabbit)
J11539 组织金属蛋白酶抑制因子3(TIMP3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) 48T/96T Bos taurus; Bovine (Cattle)
J11540 组织金属蛋白酶抑制因子3(TIMP3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) 48T/96T Capra hircus; Caprine (Goat)
J11541 组织金属蛋白酶抑制因子3(TIMP3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) 48T/96T Equus caballus; Equine (Horse)
J11542 组织金属蛋白酶抑制因子3(TIMP3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) 48T/96T Danio rerio (Zebrafish)
J11543 组织金属蛋白酶抑制因子3(TIMP3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) 48T/96T Chicken (Gallus)
J11544 组织金属蛋白酶抑制因子3(TIMP3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) 48T/96T Sus scrofa; Porcine (Pig)
J11545 组织金属蛋白酶抑制因子3(TIMP3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) 48T/96T Oryctolagus cuniculus (Rabbit)
J11546 组织金属蛋白酶抑制因子3(TIMP3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) 48T/96T Rhesus monkey (Simian)
J11547 粘蛋白6(MUC6)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Mucin 6 (MUC6) 48T/96T Homo sapiens (Human)
J11548 突触足蛋白(SYNPO)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Synaptopodin (SYNPO) 48T/96T Homo sapiens (Human)
J11549 胰岛素样生长因子结合蛋白2(IGFBP2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Insulin Like Growth Factor Binding Protein 2 (IGFBP2) 48T/96T Bos taurus; Bovine (Cattle)
J11550 胰岛素样生长因子结合蛋白2(IGFBP2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Insulin Like Growth Factor Binding Protein 2 (IGFBP2) 48T/96T Sus scrofa; Porcine (Pig)
J11551 双调蛋白(AREG)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Amphiregulin (AREG) 48T/96T Oryctolagus cuniculus (Rabbit)
J11552 窖蛋白(CAV1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Caveolin 1 (CAV1) 48T/96T Bos taurus; Bovine (Cattle)
J11553 窖蛋白(CAV1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Caveolin 1 (CAV1) 48T/96T Canis familiaris; Canine (Dog)
J11554 窖蛋白(CAV1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Caveolin 1 (CAV1) 48T/96T Capra hircus; Caprine (Goat)
J11555 窖蛋白(CAV1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Caveolin 1 (CAV1) 48T/96T Equus caballus; Equine (Horse)
J11556 窖蛋白(CAV1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Caveolin 1 (CAV1) 48T/96T Chicken (Gallus)
J11557 窖蛋白(CAV1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Caveolin 1 (CAV1) 48T/96T Mus musculus (Mouse)
J11558 窖蛋白(CAV1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Caveolin 1 (CAV1) 48T/96T Sus scrofa; Porcine (Pig)
J11559 窖蛋白(CAV1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Caveolin 1 (CAV1) 48T/96T Oryctolagus cuniculus (Rabbit)
J11560 窖蛋白(CAV1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Caveolin 1 (CAV1) 48T/96T Rhesus monkey (Simian)
J11561 转化生长因子β诱导蛋白(TGFβI)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Transforming Growth Factor Beta Induced Protein (TGFbI) 48T/96T Bos taurus; Bovine (Cattle)
J11562 转化生长因子β3(TGFβ3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Transforming Growth Factor Beta 3 (TGFb3) 48T/96T Canis familiaris; Canine (Dog)
J11563 转化生长因子β3(TGFβ3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Transforming Growth Factor Beta 3 (TGFb3) 48T/96T Capra hircus; Caprine (Goat)
J11564 转化生长因子β3(TGFβ3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Transforming Growth Factor Beta 3 (TGFb3) 48T/96T Equus caballus; Equine (Horse)
J11565 转化生长因子β诱导蛋白(TGFβI)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Transforming Growth Factor Beta Induced Protein (TGFbI) 48T/96T Danio rerio (Zebrafish)
J11566 转化生长因子β3(TGFβ3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Transforming Growth Factor Beta 3 (TGFb3) 48T/96T Chicken (Gallus)
J11567 转化生长因子β3(TGFβ3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Transforming Growth Factor Beta 3 (TGFb3) 48T/96T Cavia (Guinea pig )
J11568 转化生长因子β诱导蛋白(TGFβI)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Transforming Growth Factor Beta Induced Protein (TGFbI) 48T/96T Sus scrofa; Porcine (Pig)
J11569 转化生长因子β3(TGFβ3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Transforming Growth Factor Beta 3 (TGFb3) 48T/96T Rattus norvegicus (Rat)
J11570 转化生长因子β诱导蛋白(TGFβI)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Transforming Growth Factor Beta Induced Protein (TGFbI) 48T/96T Oryctolagus cuniculus (Rabbit)
J11571 转化生长因子β3(TGFβ3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Transforming Growth Factor Beta 3 (TGFb3) 48T/96T Rhesus monkey (Simian)
J11572 组织金属蛋白酶抑制因子4(TIMP4)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tissue Inhibitors Of Metalloproteinase 4 (TIMP4) 48T/96T Bos taurus; Bovine (Cattle)
J11573 组织金属蛋白酶抑制因子4(TIMP4)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tissue Inhibitors Of Metalloproteinase 4 (TIMP4) 48T/96T Sus scrofa; Porcine (Pig)
J11574 组织金属蛋白酶抑制因子4(TIMP4)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tissue Inhibitors Of Metalloproteinase 4 (TIMP4) 48T/96T Oryctolagus cuniculus (Rabbit)
J11575 肿瘤坏死因子α(TNFα)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tumor Necrosis Factor Alpha (TNFa) 48T/96T Capra hircus; Caprine (Goat)
J11576 肿瘤坏死因子α(TNFα)组蛋白赖氨酸N-甲基EZH1抗体检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tumor Necrosis Factor Alpha (TNFa) 48T/96T Equus caballus; Equine (Horse)
J11577 肿瘤坏死因子β(TNFβ)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tumor Necrosis Factor Beta (TNFb) 48T/96T Bos taurus; Bovine (Cattle)
J11578 肿瘤坏死因子β(TNFβ)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tumor Necrosis Factor Beta (TNFb) 48T/96T Canis familiaris; Canine (Dog)
J11579 肿瘤坏死因子β(TNFβ)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tumor Necrosis Factor Beta (TNFb) 48T/96T Sus scrofa; Porcine (Pig)
J11580 肿瘤坏死因子β(TNFβ)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tumor Necrosis Factor Beta (TNFb) 48T/96T Oryctolagus cuniculus (Rabbit)
更多组蛋白赖氨酸N-甲基EZH1抗体价格、批发价、品牌、厂家及抗体详细说明书，都可以免费咨询及索取。
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