磷酸化HER4抗体

英文名称  Anti-Phospho-HER4 (Tyr1258)

中文名称  磷酸化HER4抗体

别    名  ErbB 4 (phospho Y1258); p-ErbB 4 (phospho Y1258); ErbB4; HER 4; HER4; Avian erythroblastic leukemia viral oncogene homolog 4; Avian erythroblastic leukemia viral v erb b2 oncogene homolog 4; MGC138404; P180erbB4; Receptor protein tyrosine kinase erbB 4 precursor; Receptor tyrosine protein kinase erbB 4;Tyrosine kinase type cell surface receptor HER4; v erb a avian erythroblastic leukemia viral oncogene homolog like 4; v erb a erythroblastic leukemia viral oncogene homolog 4; Verba avian erythroblastic leukemia viral oncogene homolog like 4; Verba erythroblastic leukemia viral oncogene homolog 4; VERBB2.

磷酸化HER4抗体浓    度  1mg/1ml

规 格  0.1ml/100μg    

抗体来源  Rabbit  

克隆类型  polyclonal

交叉反应  Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, Guinea Pig

产品类型  一抗  磷酸化抗体  

研究领域  肿瘤 细胞生物 免疫学 神经生物学 信号转导 激酶和磷酸酶 细胞膜受体  

蛋白分子量  predicted molecular weight: 142kDa

性    状  Lyophilized or Liquid

免 疫 原  KLH conjugated Synthesised phosphopeptide derived from human HER4 around the phosphorylation site of Tyr1258 [PD(p-Y)LQ ]

亚    型  IgG

纯化方法  affinity purified by Protein A

储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

产品应用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500

（石蜡切片需做抗原修复）

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品介绍 This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008].

Function : Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins and EGF family members and regulates development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. Required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. Required for normal development of the embryonic central nervous system, especially for normal neural crest cell migration and normal axon guidance. Required for mammary gland differentiation, induction of milk proteins and lactation. Acts as cell-surface receptor for the neuregulins NRG1, NRG2, NRG3 and NRG4 and the EGF family members BTC, EREG and HBEGF. Ligand binding triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Ligand specificity and signaling is modulated by alternative splicing, proteolytic processing, and by the formation of heterodimers with other ERBB family members, thereby creating multiple combinations of intracellular phosphotyrosines that trigger ligand- and context-specific cellular responses. Mediates phosphorylation of SHC1 and activation of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Isoform JM-A CYT-1 and isoform JM-B CYT-1 phosphorylate PIK3R1, leading to the activation of phosphatidylinositol 3-kinase and AKT1 and protect cells against apoptosis. Isoform JM-A CYT-1 and isoform JM-B CYT-1 mediate reorganization of the actin cytoskeleton and promote cell migration in response to NRG1. Isoform JM-A CYT-2 and isoform JM-B CYT-2 lack the phosphotyrosine that mediates interaction with PIK3R1, and hence do not phosphorylate PIK3R1, do not protect cells against apoptosis, and do not promote reorganization of the actin cytoskeleton and cell migration. Proteolytic processing of isoform JM-A CYT-1 and isoform JM-A CYT-2 gives rise to the corresponding soluble intracellular domains (4ICD) that translocate to the nucleus, promote nuclear import of STAT5A, activation of STAT5A, mammary epithelium differentiation, cell proliferation and activation of gene expression. The ERBB4 soluble intracellular domains (4ICD) colocalize with STAT5A at the CSN2 promoter to regulate transcription of milk proteins during lactaction. The ERBB4 soluble intracellular domains can also translocate to mitochondria and promote apoptosis.

Subunit : Monomer in the absence of bound ligand. Homodimer or heterodimer with another ERBB family member upon ligand binding, thus forming heterotetramers. Interacts with EGFR and ERBB2. Interacts with CBFA2T3 (By similarity). Interacts with DLG2 (via its PDZ domain), DLG3 (via its PDZ domain), DLG4 (via its PDZ domain) and SNTB2 (via its PDZ domain). Interacts with MUC1. Interacts (via its PPxy motifs) with WWOX. Interacts (via the PPxY motif 3 of isoform JM-A CYT-2) with YAP1 (via the WW domain 1 of isoform 1). Interacts (isoform JM-A CYT-1 and isoform JM-B CYT-1) with WWP1. Interacts (via its intracellular domain) with TRIM28. Interacts (via the intracellular domains of both CYT-1 and CYT-2 isoforms) with KAP1; the interaction does not phosphorylate KAP1 but represses ERBB4-mediated transcriptional activity. Interacts with PRPU, DDX23, MATR3, RBM15, ILF3, KAP1, U5S1, U2SURP, ITCH, HNRPU, AP2A1, NULC, LEO1, WWP2, IGHG1, HXK1, GRB7 AND ARS2. Interacts (phosphorylated isoform JM-A CYT-1 and isoform JM-B CYT-1) with PIK3R1. Interacts with SHC1. Interacts with GRB2. Interacts (soluble intracellular domain) with STAT5A. Interacts (soluble intracellular domain) with BCL2. Interacts (phosphorylated) with STAT1.

Subcellular Location : Cell membrane; Single-pass type I membrane protein. Note=In response to NRG1 treatment, the activated receptor is internalized. ERBB4 intracellular domain: Nucleus. Mitochondrion. Note=Following proteolytical processing E4ICD (E4ICD1 or E4ICD2 generated from the respective isoforms) is translocated to the nucleus. Significantly more E4ICD2 than E4ICD1 is found in the nucleus. E4ICD2 colocalizes with YAP1 in the nucleus.

Tissue Specificity : Expressed at highest levels in brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis and breast. Lower levels in thymus, lung, salivary gland, and pancreas. Isoform JM-A CYT-1 and isoform JM-B CYT-1 are expressed in cerebellum, but only the isoform JM-B is expressed in the heart.

Post-translational modifications : Isoform JM-A CYT-1 and isoform JM-A CYT-2 are processed by ADAM17. Proteolytic processing in response to ligand or 12-O-tetradecanoylphorbol-13-acetate stimulation results in the production of 120 kDa soluble receptor forms and intermediate membrane-anchored 80 kDa fragments (m80HER4), which are further processed by a presenilin-dependent gamma-secretase to release a cytoplasmic intracellular domain (E4ICD; E4ICD1/s80Cyt1 or E4ICD2/s80Cyt2, depending on the isoform). Membrane-anchored 80 kDa fragments of the processed isoform JM-A CYT-1 are more readily degraded by the proteasome than fragments of isoform JM-A CYT-2, suggesting a prevalence of E4ICD2 over E4ICD1. Isoform JM-B CYT-1 and isoform JM-B CYT-2 lack the ADAM17 cleavage site and are not processed by ADAM17, precluding further processing by gamma-secretase.

Similarity : Belongs to the protein kinase superfamily.

Tyr protein kinase family.

EGF receptor subfamily.

Contains 1 protein kinase domain.

Database links : UniProtKB/Swiss-Prot: Q15303.1

半个多世纪以来，抗体一直作为常用的研究工具，因此对其使用和储存已有丰富的经验。这些经验让人们知道如何对抗体进行操作，由于抗体的稳定性好，使用并不困难。抗体较其他蛋白不易变性，易于存放、纯化和标记。蛋白G和蛋白A等抗体结合蛋白的发现使得这项工作更具可行性。

J04659 兔子凝血因子Ⅱ(FⅡ)ELISA Kit，48T/96T

J04660 鱼类超敏C反应蛋白(hs-CRP)ELISA Kit，48T/96T

J04661 人超敏C反应蛋白(hs-CRP)ELISA Kit，48T/96T

J04662 小鼠超敏C反应蛋白(hs-CRP)ELISA Kit，48T/96T

J04663 猴超敏C反应蛋白(hs-CRP)ELISA Kit，48T/96T

J04664 大鼠超敏C反应蛋白(hs-CRP)ELISA Kit，48T/96T

J04665 兔超敏C反应蛋白(hs-CRP)ELISA Kit，48T/96T

J04666 鸡热休克蛋白60(Hsp-60)ELISA Kit，48T/96T

J04667 人热休克蛋白60(Hsp-60)ELISA Kit，48T/96T

J04668 小鼠热休克蛋白60(Hsp-60)ELISA Kit，48T/96T

J04669 大鼠热休克蛋白60(Hsp-60)ELISA Kit，48T/96T

J04670 人热休克蛋白糖蛋白96(HSP gp96)ELISA Kit，48T/96T

J04671 小鼠热休克蛋白糖蛋白96(HSP gp96)ELISA Kit，48T/96T

J04672 猴子热休克蛋白糖蛋白96(HSP gp96)ELISA Kit，48T/96T

J04673 大鼠热休克蛋白糖蛋白96(HSP gp96)ELISA Kit，48T/96T

J04674 牛可溶性白细胞分化抗原86(B7-2/sCD86)ELISA Kit，48T/96T

J04675 人可溶性白细胞分化抗原86(B7-2/sCD86)ELISA Kit，48T/96T

J04676 小鼠可溶性白细胞分化抗原86(B7-2/sCD86)ELISA Kit，48T/96T

J04677 大鼠可溶性白细胞分化抗原86(B7-2/sCD86)ELISA Kit，48T/96T

J04678 人缺血修饰白蛋白(IMA)ELISA Kit，48T/96T

J04679 小鼠缺血修饰白蛋白(IMA)ELISA Kit，48T/96T

J04680 人凝血酶受体(TR)ELISA Kit，48T/96T

J04681 小鼠凝血酶受体(TR)ELISA Kit，48T/96T

J04682 大鼠凝血酶受体(TR)ELISA Kit，48T/96T

J04683 兔子凝血酶受体(TR)ELISA Kit，48T/96T

J04684 蛇毒因子(CVF)ELISA Kit，48T/96T

J04685 人抗凝血酶受体(ATR)ELISA Kit，48T/96T

J04686 小鼠抗凝血酶受体(ATR)ELISA Kit，48T/96T

J04687 猪抗凝血酶受体(ATR)ELISA Kit，48T/96T

J04688 大鼠抗凝血酶受体(ATR)ELISA Kit，48T/96T

J04689 牛C反应蛋白(CRP)ELISA Kit，48T/96T

J04690 鸡C反应蛋白(CRP)ELISA Kit，48T/96T

J04691 人C反应蛋白(CRP)ELISA Kit，48T/96T

J04692 小鼠C反应蛋白(CRP)ELISA Kit，48T/96T

J04693 猪C反应蛋白(CRP)ELISA Kit，48T/96T

J04694 大鼠C反应蛋白(CRP)ELISA Kit，48T/96T

J04695 兔子C反应蛋白(CRP)ELISA Kit，48T/96T

J04696 犬促卵泡素(FSH)ELISA Kit，48T/96T

J04697 鸡促卵泡素(FSH)ELISA Kit，48T/96T

J04698 豚鼠促卵泡素(FSH)ELISA Kit，48T/96T

J04699 人促卵泡素(FSH)ELISA Kit，48T/96T

J04700 小鼠促卵泡素(FSH)ELISA Kit，48T/96T

J04701 猴子促卵泡素(FSH)ELISA Kit，48T/96T

J04702 猪促卵泡素(FSH)ELISA Kit，48T/96T

J04703 大鼠促卵泡素(FSH)ELISA Kit，48T/96T

J04704 兔子促卵泡素(FSH)ELISA Kit，48T/96T

J04705 人凝血酶抗凝血酶复合物(TAT)ELISA Kit，48T/96T

J04706 小鼠凝血酶抗凝血酶复合物(TAT)磷酸化HER4抗体ELISA Kit，48T/96T

J04707 大鼠凝血酶抗凝血酶复合物(TAT)ELISA Kit，48T/96T

J04708 豚鼠纤溶酶抗纤溶酶复合物(PAP)ELISA Kit，48T/96T

J04709 人纤溶酶抗纤溶酶复合物(PAP)ELISA Kit，48T/96T

J04710 小鼠纤溶酶抗纤溶酶复合物(PAP)ELISA Kit，48T/96T

J04711 大鼠纤溶酶抗纤溶酶复合物(PAP)ELISA Kit，48T/96T

J04712 兔纤溶酶抗纤溶酶复合物(PAP)ELISA Kit，48T/96T

J04713 人血管性血友病因子/瑞斯托霉素辅因子(VWF)ELISA Kit，48T/96T

J04714 小鼠血管性血友病因子/瑞斯托霉素辅因子(vWF)

更多磷酸化HER4抗体价格、批发价、品牌、厂家及抗体详细说明书，都可以免费咨询及索取。