效率的因素主要有5个方面:①抗体对抗原的亲合性;②抗体对抗原的特异性;③抗原表位结构在该技术中是如何被改变的;④抗体与抗原结合的易接近性;⑤二级试剂的质量和类型
英文名称 Anti-phospho-Ephrin B(Ser287)
中文名称 磷酸化Ephrin B抗体
别 名 Ephrin B (phospho S287); p-Ephrin B (phospho S287); CEK5 ligand; EFNB1; ELK L; EphrinB; Ephrin-B; ELK ligand; ELKL; EPH related receptor tyrosine kinase ligand 2; Ephrin B Precursor; Ephrin B1; Ephrin B2; LERK 2; LERK2
磷酸化Ephrin B抗体浓 度 1mg/1ml
规 格 0.1ml/100μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Chicken, Xenopus laevis
产品类型 一抗 磷酸化抗体
研究领域 肿瘤 细胞生物 神经生物学 生长因子和激素
蛋白分子量 predicted molecular weight: 34kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human Ephrin B around the phosphorylation site of Ser287
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 Ephrin B proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development. Ephrin B molecules expressed at the membrane surface bind to the Ephrin B family receptors on target cells during cell to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing Ephrin B on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of Ephrin B is thought to be a critical component of this reverse signaling event. Recent work demonstrated that Tyr331 of Ephrin B was phosphorylated in HEK293 cells after stimulation by the soluble Ephrin B2 receptor tyrosine kinase.
Subunit : Interacts with GRIP1 and GRIP2. Binds to Nipah virus G protein.
Subcellular Location : Membrane; Single-pass type I membrane protein.
Tissue Specificity : Highly expressed in brain; expressed in embryonic floor plate, roof plate and hindbrain segments.
Similarity : Belongs to the ephrin family.
Contains 1 ephrin RBD (ephrin receptor-binding) domain.
Database links : UniProtKB/Swiss-Prot: P98172.1 human
UniProtKB/Swiss-Prot: P52799.1 human
半个多世纪以来,抗体一直作为常用的研究工具,因此对其使用和储存已有丰富的经验。这些经验让人们知道如何对抗体进行操作,由于抗体的稳定性好,使用并不困难。抗体较其他蛋白不易变性,易于存放、纯化和标记。蛋白G和蛋白A等抗体结合蛋白的发现使得这项工作更具可行性。
选择合适的抗体对免疫化学技术的成功极为重要。没有任何一种来源的抗体能够满足本书所述的各种方法的要求。为了研究某一重要的抗原,应选用具有不同特性的多种抗体。在没有一套良好特性的抗体可用于研究机体中每一种蛋白之前,选择及制备适合各种技术要求的抗体非常必要。
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