效率的因素主要有5个方面：①抗体对抗原的亲合性；②抗体对抗原的特异性；③抗原表位结构在该技术中是如何被改变的；④抗体与抗原结合的易接近性；⑤二级试剂的质量和类型
英文名称 Anti-EDC4
中文名称 自身抗原EDC4抗体
别 名 autoantigen; Autoantigen Ge 1; Autoantigen Ge-1; Autoantigen Ge1; Autoantigen RCD 8; Autoantigen RCD-8; Autoantigen RCD8; edc4; EDC4_HUMAN; Enhancer of mRNA decapping 4; Enhancer of mRNA-decapping protein 4; Ge 1; Hedls; Human enhancer of decapping large subunit; RCD 8.
自身抗原EDC4抗体浓 度 1mg/1ml
规 格 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat
产品类型 一抗
研究领域 细胞生物 免疫学
蛋白分子量 predicted molecular weight: 151kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human EDC4
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 The major eukaryotic mRNA decay pathway occurs through deadenylation, decapping, and 5' to 3' degradation of the mRNA. Decapping is a critical control point in this decay pathway. Edc4 (enhancer of mRNA decapping 4), also known as human enhancer of decapping large subunit (HEDLS), RCD-8 or Ge-1, is a 1,401 amino acid protein belonging to the WD repeat EDC4 family that is involved in mRNA decapping during mRNA degradation. As part of the mRNA degradation process, Edc4 becomes part of a complex that also contains hDcp1a, hDcp2a, RCK and Edc3. Localizing to P-body and cytoplasm, Edc4 contains a nuclear localization sequence (NLS) which enables it to selectively enter the nucleus as well. Edc4 becomes phosphorylated upon DNA damage and exists as two alternatively spliced isoforms that are encoded by a gene that maps to human chromosome 16q22.1.
Function : In the process of mRNA degradation, seems to play a role in mRNA decapping. Component of a complex containing DCP2 and DCP1A which functions in decapping of ARE-containing mRNAs. Promotes complex formation between DCP1A and DCP2. Enhances the catalytic activity of DCP2 (in vitro).
Subunit : Part of a decapping complex consisting of DCP1A, DCP2, EDC3, EDC4 and probably DDX6. Part of a complex consisting of DCP1A, EDC3, EDC4 and DDX6. Part of a complex consisting of DCP1B, EDC3, EDC4 and DDX6. Interacts with DCP2.
Subcellular Location : Cytoplasm; P-body. Nucleus.
Post-translational modifications : Phosphorylated upon DNA damage, probably by ATM or ATR.
Similarity : Belongs to the WD repeat EDC4 family.
Contains 4 WD repeats.
Database links : UniProtKB/Swiss-Prot: Q6P2E9.1
半个多世纪以来，抗体一直作为常用的研究工具，因此对其使用和储存已有丰富的经验。这些经验让人们知道如何对抗体进行操作，由于抗体的稳定性好，使用并不困难。抗体较其他蛋白不易变性，易于存放、纯化和标记。蛋白G和蛋白A等抗体结合蛋白的发现使得这项工作更具可行性。
选择合适的抗体对免疫化学技术的成功极为重要。没有任何一种来源的抗体能够满足本书所述的各种方法的要求。为了研究某一重要的抗原，应选用具有不同特性的多种抗体。在没有一套良好特性的抗体可用于研究机体中每一种蛋白之前，选择及制备适合各种技术要求的抗体非常必要。
J02039 小鼠催乳素(PRL)ELISA试剂盒 mouse prolactin,PRL ELISA Kit 96T/48T
J02040 小鼠抵抗素(Resistin)ELISA试剂盒 Mouse Resistin ELISA Kit 96T/48T
J02041 小鼠乙酰胆碱受体抗体(AChRab)ELISA试剂盒 Mouse acetylcholine receptor antibody,AChRab ELISA Kit 96T/48T
J02042 小鼠骨钙素/骨谷氨酸蛋白(OT/BGP)ELISA试剂盒 Mouse Osteocalcin/Bone gla protein,OT/BGP ELISA Kit 96T/48T
J02043 小鼠γ氨基丁酸(GABA)ELISA试剂盒 Mouse Gamma-aminobutyric acid,GABA ELISA Kit 96T/48T
J02044 小鼠5羟色胺(5-HT)ELISA试剂盒 Mouse 5-Hydroxytryptamine,5HT ELISA Kit 96T/48T
J02045 小鼠P物质受体(SP-R)ELISA试剂盒 Mouse substance P receptor,SP-R ELISA Kit 96T/48T
J02046 小鼠P物质(SP)ELISA试剂盒 Mouse Substance P,SP ELISA Kit 96T/48T
J02047 小鼠血管活性肠肽(VIP)ELISA试剂盒 Mouse Vasoactive Intestinal Peptide,VIP ELISA Kit 96T/48T
J02048 小鼠甲状腺过氧化物酶(TPO)ELISA试剂盒 Mouse Thyroid-Peroxidase,TPO ELISA Kit 96T/48T
J02049 小鼠αL岩藻糖苷酶(AFU)ELISA试剂盒 Mouse alpha-L-fucosidase,AFU ELISA Kit 96T/48T
J02050 小鼠血管舒缓激肽(BK)ELISA试剂盒 Mouse bradykinin,BK ELISA Kit 96T/48T
J02051 小鼠胰岛素原(PI)ELISA试剂盒 Mouse Proinsulin,PI ELISA Kit 96T/48T
J02052 小鼠抗IgE受体抗体ELISA试剂盒 Mouse anti-IgE receptor antibody ELISA Kit 96T/48T
J02053 小鼠抗IVIgG抗体ELISA试剂盒 Mouse anti-IVIgG antibody ELISA Kit 96T/48T
J02054 小鼠抗心肌抗体(AMA)ELISA试剂盒 Mouse Anti-myocardial antibody,AMA ELISA Kit 96T/48T
J02055 小鼠铜蓝蛋白(CP/CER)ELISA试剂盒 Mouse Ceruloplasmin,CP/CER ELISA Kit 96T/48T
J02056 小鼠p53(p53)ELISA试剂盒 Mouse p53/tumor protein,p53/TP53 ELISA Kit 96T/48T
J02057 小鼠环磷酸鸟苷(cGMP)ELISA试剂盒 Mouse Cyclic guanosine monophosphate,cGMP ELISA Kit 96T/48T
J02058 小鼠一氧化氮合成酶(NOS)ELISA试剂盒 Mouse nitric oxide synthase,NOS ELISA Kit 96T/48T
J02059 小鼠诱导型一氧化氮合成酶(iNOS)ELISA试剂盒 Mouse inducible nitric oxide synthase,iNOS ELISA Kit 96T/48T
J02060 小鼠内皮型一氧化氮合成酶(eNOS)ELISA试剂盒 Mouse Endothelial nitric oxide synthase,eNOS ELISA Kit 96T/48T
J02061 小鼠组胺(HIS)ELISA试剂盒 Mouse Histamine,HIS ELISA Kit 96T/48T
J02062 小鼠脂蛋白相关磷脂酶A2(Lp-PL-A2)ELISA试剂盒 Mouse lipoprotein-associated phospholipase A2,Lp-PL-A2 ELISA Kit 96T/48T
J02063 小鼠热休克蛋白90(HSP-90)ELISA试剂盒 Mouse Heat Shock Protein 90,Hsp-90 ELISA Kit 96T/48T
J02064 小鼠热休克蛋白70(HSP-70)ELISA试剂盒 Mouse Heat Shock Protein 70,Hsp-70 ELISA Kit 96T/48T
J02065 小鼠脂联素(ADP)ELISA试剂盒 Mouse adiponectin,ADP ELISA Kit 96T/48T
J02066 小鼠热休克蛋白40(Hsp-40)ELISA试剂盒 Mouse Heat Shock Protein 40,Hsp-40 ELISA Kit 96T/48T
J02067 小鼠β淀粉样蛋白1-40(Aβ1-40)ELISA试剂盒 Mouse amyloid beta peptide 1-40,Aβ1-40 ELISA Kit 96T/48T
J02068 小鼠白介素8(IL-8/CXCL8)ELISA试剂盒 Mouse interleukin 8,IL-8 ELISA Kit 96T/48T
J02069 小鼠细胞周期素D3(Cyclin-D3)ELISA试剂盒 Mouse Cyclin-D3 ELISA Kit 96T/48T
J02070 小鼠细胞周期素D2(Cyclin-D2)ELISA试剂盒 Mouse Cyclin-D2 ELISA Kit 96T/48T
J02071 小鼠雌激素(E)ELISA试剂盒 Mouse estrogen,E ELISA Kit 96T/48T
J02072 小鼠细胞周期素D1(Cyclin-D1)ELISA试剂盒 Mouse Cyclin-D1 ELISA Kit 96T/48T
J02073 小鼠髓磷脂碱性蛋白(MBP)ELISA试剂盒 Mouse myelin basic protein,MBP ELISA Kit 96T/48T
J02074 小鼠转化生长因子α(TGF-α)ELISA试剂盒 Mouse transforming growth factor α,TGF-α ELISA Kit 96T/48T
J02075 小鼠甲种胎儿球蛋白/甲胎蛋白(AFP)ELISA试剂盒 Mouse Alpha-fetoprotein,AFP ELISA Kit 96T/48T
J02076 小鼠前列腺酸性磷酸酶(PAP)ELISA试剂盒 Mouse Prostatic Acid Phosphatase,PAP ELISA Kit 96T/48T
J02077 小鼠前列腺特异性抗原(PSA)ELISA试剂盒 Mouse prostate specific antigen,PSA ELISA Kit 96T/48T
J02078 小鼠卵巢癌标志物CA125ELISA试剂盒 Mouse ovarian cancer marker-CA125 ELISA Kit 96T/48T
J02079 小鼠乳腺癌标志物-CA153ELISA试剂盒 Mouse mammary carcinoma Marker-CA153 ELISA Kit 96T/48T
J02080 小鼠胃肠癌标志物CA199ELISA试剂盒 Mouse Gastrointestinalcancer Marker-CA199 ELISA Kit 96T/48T
J02081 小鼠血红素氧合酶1(HO-1)ELISA试剂盒 Mouse heme oxygenase 1,HO-1 ELISA Kit 96T/48T
J02082 小鼠磷脂酶A2(PL-A2)ELISA试剂盒 Mouse Phospholipidase A2,PL-A2 ELISA Kit 96T/48T
J02083 小鼠组织因子途径抑制物(TFPI)ELISA试剂盒 Mouse Tissue factor pathway inhibitor,TFPI ELISA Kit 96T/48T
J02084 小鼠水通道蛋白5(AQP-5)ELISA试剂盒 Mouse Aquaporin 5,AQP-5 ELISA Kit 96T/48T
J02085 小鼠水通道蛋白4(AQP-4)ELISA试剂盒 Mouse Aquaporin 4,AQP-4 ELISA Kit 96T/48T
J02086 小鼠水通道蛋白3(AQP-3)ELISA试剂盒 Mouse Aquaporin 3,AQP-3 ELISA Kit 96T/48T
J02087 小鼠水通道蛋白1(AQP-1)ELISA试剂盒 Mouse Aquaporin 1,AQP-1 ELISA Kit 96T/48T
J02088 小鼠水通道蛋白0(AQP-0)ELISA试剂盒 Mouse Aquaporin 0,AQP-0 ELISA Kit 96T/48T
J02089 小鼠水通道蛋白2(AQP-2)ELISA试剂盒 Mouse Aquaporin 2,AQP-2 ELISA Kit 96T/48T
J02090 小鼠甲状腺球蛋白(TG)ELISA试剂盒 Mouse Thyroglobulin,TG ELISA Kit 96T/48T
J02091 小鼠抑制素A(INH-A)ELISA试剂盒 Mouse Inhibin,INH-A ELISA Kit 96T/48T
J02092 小鼠绒毛膜促性腺激素β(β-CG)ELISA试剂盒 Mouse Chorionic Gonadotrophin β,β-CG ELISA Kit 96T/48T
J02093 小鼠性激素结合球蛋白(SHBG)ELISA试剂盒 Mouse sex hormone-binding globulin,SHBG ELISA Kit 96T/48T
J02094 小鼠脱氢表雄酮S7(DHEA-S7)ELISA试剂盒 自身抗原EDC4抗体Mouse Dehydroepiandrosterone S7,DHEA-S7 ELISA Kit 96T/48T
J02095 小鼠脱氢表雄酮(DHEA)ELISA试剂盒 Mouse Dehydroepiandrosterone,DHEA ELISA Kit 96T/48T
J02096 小鼠高迁移率族蛋白B1(HMGB-1)ELISA试剂盒 Mouse High mobility group protein B1,HMGB-1 ELISA Kit 96T/48T
J02097 小鼠内皮脂肪酶(EL)ELISA试剂盒 Mouse Endothelial lipase,EL ELISA Kit 96T/48T
J02098 小鼠碳酸酐酶(CA)ELISA试剂盒 Mouse carbonic anhydrase,CA ELISA Kit 96T/48T
更多自身抗原EDC4抗体价格、批发价、品牌、厂家及抗体详细说明书，都可以免费咨询及索取。
关注
已认证
