核酸外切酶1抗体

英文名称  Anti-Exonuclease 1

中文名称  核酸外切酶1抗体

别    名  exo1; EXO1_HUMAN; ExoI; Exonuclease 1; Exonuclease I; Exonuclease1; HEX1; hExo I; hExo1; hExoI; Rad2 nuclease family member homolog of S. cerevisiae exonuclease 1.

核酸外切酶1抗体浓    度  1mg/1ml

规 格  0.2ml/200μg    

抗体来源  Rabbit  

克隆类型  polyclonal

交叉反应  Human, Mouse, Rat, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster, Orangutan  

产品类型  一抗    

研究领域  细胞生物 表观遗传学  

蛋白分子量  predicted molecular weight: 94kDa

性    状  Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human Exonuclease 1

亚    型  IgG

纯化方法  affinity purified by Protein A

储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

产品应用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500

（石蜡切片需做抗原修复）

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品介绍 Comparative evaluation of the expression patterns of the human and mouse genes, combined with previous biochemical and yeast genetic studies, indicate that the Exo1 (Exonuclease I) proteins are important contributors to chromosome processing during mammalian DNA repair and recombination. In mice, the Exo1 gene maps to distal chromosome 1, consistent with the recent mapping of the orthologous human HEX1/EXO1 gene to chromosome 1q43. Exo1 is expressed prominently in testis, an area of active homologous recombination, and spleen, a prominent lymphoid tissue. In both mammalian and yeast systems, Exo1 is a 5'-3' double stranded DNA exonuclease that has previously been implicated in DNA mismatch repair (MMR). The MMR system ensures genome integrity by removing mispaired and unpaired bases that originate during replication. In humans, Exo1 interacts with MSH2 and MLH1 and has been proposed to be a redundant exonuclease in MMR. In both mammalian and yeast systems, Exo1 plays a structural role in MMR and stabilizes multiprotein complexes containing a number of MMR proteins.

Function : 5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis.

Subunit : Interacts with the MLH1-PMS2 heterodimer via MLH1. Interacts with MSH3. Interacts with the MSH2-MSH6 heterodimer via MSH2, and this interaction may increase the processivity of the 5'->3' exonuclease activity. Interacts with PCNA, and this interaction may both stimulate the cryptic 3'->5' exonuclease activity and suppress the 5'->3' exonuclease activity. Interacts with WRN, and this interaction stimulates both the 5'->3' exonuclease activity and cleavage of 5'-overhanging flap structures. Interacts with RECQL/RECQ1, and this interaction stimulates cleavage of 5'-overhanging flap structures.

Subcellular Location : Nucleus. Colocalizes with PCNA to discrete nuclear foci in S-phase.

Tissue Specificity : Highly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach.

Post-translational modifications : Phosphorylated upon DNA damage and in response to agents stalling DNA replication, probably by ATM or ATR. Phosphorylation at Ser-454, Thr-621 and Ser-714 is induced upon DNA-damage caused by treatment with hydroxyurea (HU) but not upon IR treatment. The HU-induced EXO1 triple phosphorylation facilitates destabilisation/degradation of the protein.

Similarity : Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily.

Database links : UniProtKB/Swiss-Prot: Q9UQ84.2

半个多世纪以来，抗体一直作为常用的研究工具，因此对其使用和储存已有丰富的经验。这些经验让人们知道如何对抗体进行操作，由于抗体的稳定性好，使用并不困难。抗体较其他蛋白不易变性，易于存放、纯化和标记。蛋白G和蛋白A等抗体结合蛋白的发现使得这项工作更具可行性。

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J00982 人羟赖氨酸(Hyl)ELISA试剂盒 Human hydroxylysine,Hyl ELISA Kit 96T/48T

J00983 人GTP酶活化蛋白(GAP)ELISA试剂盒 Human GTPase activating protein,GAP ELISA Kit 96T/48T

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J00985 人过氧化物酶体增殖物激活受体α(PPAR-α)ELISA试剂盒 Human peroxisome proliferators activator receptors alpha,PPAR-α ELISA Kit 96T/48T

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J00987 人羟基胶原吡啶交联(OH-PYD)ELISA试剂盒 Human Hydroxy-Pyridinium crosslinks,OH-PYD ELISA Kit 96T/48T

J00988 人脱氧胶原吡啶交联(DPD)ELISA试剂盒 Human Deoxypyridinoline crosslinks,DPD ELISA Kit 96T/48T

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J00991 人刀豆素A(ConA)ELISA试剂盒 Human concanavalin A,ConA ELISA Kit 96T/48T

J00992 人游离原卟啉(FEP)ELISA试剂盒 Human Free erythrocyte proloporphyrin,FEP ELISA Kit 96T/48T

J00993 人脂多糖结合蛋白(LBP)ELISA试剂盒 Human lipolysaccharide binding protein,LBP ELISA Kit 96T/48T

J00994 人谷胱甘肽过氧化酶(GSH-Px)ELISA试剂盒 Human Glutathione peroxidase,GSH-Px ELISA Kit 96T/48T

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J00996 人蛋白脂质蛋白抗体(PLP)ELISA试剂盒 Human proteolipid protein,PLP ELISA Kit 96T/48T

J00997 人垂草扁桃酸(VMA) ELISA试剂盒 Human vanillylmandelic acid,VMA ELISA Kit 96T/48T

J00998 人葡萄糖激酶调节蛋白(GKRP)ELISA试剂盒 Human glucokinase regulatory protein,GKRP ELISA Kit 96T/48T

J00999 人骨退化特异标志物(CTX-2)ELISA试剂盒 Human CTX-2 ELISA Kit 96T/48T

J01000 人钙结合蛋白(CR)ELISA试剂盒 Human Calretinin,CR ELISA Kit 96T/48T

J01001 人骨形成蛋白6(BMP-6)ELISA试剂盒 Human Bone Morphogenetic Protein 6,BMP-6 ELISA Kit 96T/48T

J01002 人脂肪酸结合蛋白(FABP)ELISA试剂盒 Human fatty acid-binding protein,FABP ELISA Kit 96T/48T

J01003 人腺苷三磷酸结合盒转运体A1(ABCA1)ELISA试剂盒 Human ATP-binding cassette transporter A1,ABCA1 ELISA Kit 96T/48T

J01004 人羟脯氨酸糖蛋白(HRGP)ELISA试剂盒 Human Hydroxyproline-Rich Glycoprotein,HRGP ELISA Kit 96T/48T

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J01007 人骨涎蛋白(BSP)ELISA试剂盒 Human bone sialoprotein,BSP ELISA Kit 96T/48T

J01008 人1,3-二磷酸甘油酸(1,3-DPG)ELISA试剂盒 Human 1,3-Disphosphoglycerate,1,3-DPG ELISA Kit 96T/48T

J01009 人1,5-脱水葡萄糖醇/1,5-脱水山梨(1,5-AG)ELISA试剂盒 Human 1,5-anhydroglucitol,1,5-AG ELISA Kit 96T/48T

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J01011 人低密度脂蛋白受体相关蛋白6(LRP-6)ELISA试剂盒 Human low-density lipoprotein-receptor-related protein,LRP-6 ELISA Kit 96T/48T

J01012 人极低密度脂蛋白受体(VLDLR)ELISA试剂盒 Human very low density lipoprotein receptor,VLDLR ELISA Kit 96T/48T

J01013 人低密度脂蛋白受体(LDLR)ELISA试剂盒 Human very low density lipoprotein receptor,VLDLR ELISA Kit 96T/48T

J01014 人过氧化物酶体增殖因子活化受体γ( PPAR-γ)ELISA试剂盒 Human Peroxisome Proliferator-activated receptor γ,PPAR-γ ELISA Kit 96T/48T

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J01016 人15脂加氧酶(15-LO/LOX)ELISA试剂盒 Human 15-lipoxygenase,15-LO/LOX ELISA Kit 96T/48T

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J01018 人糖原磷酸化酶同工酶II(GP-II)ELISA试剂盒 Human Glycogen phosphorylase II,GP-II ELISA Kit 96T/48T

J01019 人糖原磷酸化酶同工酶MM(GP-MM)ELISA试剂盒 Human Glycogen phosphorylase MM,GP-MM ELISA Kit 96T/48T

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J01026 人内皮脂肪酶(EL)ELISA试剂盒 Human Endothelial lipase,EL ELISA Kit 96T/48T

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