效率的因素主要有5个方面：①抗体对抗原的亲合性；②抗体对抗原的特异性；③抗原表位结构在该技术中是如何被改变的；④抗体与抗原结合的易接近性；⑤二级试剂的质量和类型
英文名称 Anti-Phospho-ELk1(Ser383)
中文名称 磷酸化细胞转录因子ELK1抗体
别 名 ELK1 (phospho S383); Elk-1(Phospho-Ser383); p-ELK1 (S383); p-Elk-1(Ser383); ELK 1; ELK1 member of ETS oncogene family; ELK1 protein; ELK2 member of ETS oncogene family; ETS domain containing protein Elk 1; ETS domain containing protein Elk1; ETS domain protein Elk1; Oncogene Elk1; ETS domain-containing protein Elk-1 isoform a; ETS domain-containing protein Elk-1; ETS-like gene 1; tyrosine kinase (ELK1) oncogene; ELK1_HUMAN.
磷酸化细胞转录因子ELK1抗体浓 度 1mg/1ml
规 格 0.1ml/100μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit
产品类型 一抗 磷酸化抗体
研究领域 肿瘤 转录调节因子 激酶和磷酸酶 表观遗传学
蛋白分子量 predicted molecular weight: 47kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthesised phosphopeptide derived from human ELk1 around the phosphorylation site of Ser383
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14. [provided by RefSeq, Mar 2012].
Function : Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.
Subunit : Interacts in its sumoylated form with PIAS2/PIASX which enhances its transcriptional activator activity. Interacts with MAD2L2; the interaction is direct and promotes phosphorylation by the kinases MAPK8 and/or MAPK9.
Subcellular Location : Nucleus.
Tissue Specificity : Lung and testis.
Post-translational modifications : Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Also phosphorylated on Ser-383 by MAPK8 and/or MAKP9. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity. Phosphorylated and activated by CAMK4, MAPK11, MAPK12 and MAPK14.
Similarity : Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Database links : UniProtKB/Swiss-Prot: P19419.2
半个多世纪以来，抗体一直作为常用的研究工具，因此对其使用和储存已有丰富的经验。这些经验让人们知道如何对抗体进行操作，由于抗体的稳定性好，使用并不困难。抗体较其他蛋白不易变性，易于存放、纯化和标记。蛋白G和蛋白A等抗体结合蛋白的发现使得这项工作更具可行性。
选择合适的抗体对免疫化学技术的成功极为重要。没有任何一种来源的抗体能够满足本书所述的各种方法的要求。为了研究某一重要的抗原，应选用具有不同特性的多种抗体。在没有一套良好特性的抗体可用于研究机体中每一种蛋白之前，选择及制备适合各种技术要求的抗体非常必要。
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J00742 人天门冬氨酸转氨酶/谷草转氨酶(GOT/GPT)ELISA试剂盒 Human Aspartate aminotransferase,GOT/GPT ELISA Kit 96T/48T
J00743 人纤维连接素相关抗原(FRA)ELISA试剂盒 Human fibronection related antigen,FRA ELISA Kit 96T/48T
J00744 人胃泌素细胞抗体(GCA)ELISA试剂盒 Human gastrin cell antibody,GCA ELISA Kit 96T/48T
J00745 人肝素辅因子Ⅱ(HCⅡ)ELISA试剂盒 Human heparin cofactor Ⅱ,HCⅡELISA Kit 96T/48T
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J00748 人胃泌素释放多肽(GRP)ELISA试剂盒 Human gastrin-reliasing peptide,GRP ELISA Kit 96T/48T
J00749 人胃泌素释放肽前体(ProGRP)ELISA试剂盒 Human pro-gastrin-releasing peptide, ProGRP ELISA Kit 96T/48T
J00750 人促胰液素/胰泌素(Secretin)ELISA试剂盒 Human Secretin ELISA Kit 96T/48T
J00751 人胰蛋白酶原激活肽(TAP)ELISA Human trypsinogen activation peptide,TAP ELISA Kit 96T/48T
J00752 人内皮型一氧化氮合成酶3(eNOS-3)ELISA试剂盒 Human α1-Acid glycoprotein,α1-AGP ELISA Kit 96T/48T
J00753 人胰淀素(Amylin)ELISA试剂盒 Human Amylin ELISA Kit 96T/48T
J00754 人血管活性肠肽(VIP)ELISA试剂盒 Human Motilin,MTL ELISA Kit 96T/48T
J00755 人NOGO-A抗体(Nogo-A Ab)ELISA试剂盒 Human anti-Nogo-A antibody,NOGO-A Ab ELISA Kit 96T/48T
J00756 人抗组织转谷氨酰胺酶抗体IgA(tTG-IgA)ELISA试剂盒 Human Anti-tissue tranGSlutaminase IgA,tTG-IgA ELISA Kit 96T/48T
J00757 人抗存活素抗体/生存蛋白(Surv)ELISA试剂盒 Human anti-Survivin antibody,Surv ELISA Kit 96T/48T
J00758 人粒细胞巨噬细胞集落刺激因子抗体(GM-CSF Ab)ELISA试剂盒 Human anti-Granulocyte-Macrophage Colony Stimulating Factor antibody,GM-CSF Ab ELISA Kit 96T/48T
J00759 人抗肌联蛋白抗体(TTN)ELISA试剂盒 Human Anti-titin Antibody,TTN ELISA Kit 96T/48T
J00760 人抗突触前膜抗体(PsmAb)ELISA试剂盒 Human anti-presynaptic membrane antibody,PsmAb ELISA Kit 96T/48T
J00761 人血管紧张素Ⅱ受体2抗体(AT2R-Ab)ELISA试剂盒 Human Angiotensin Ⅱ Receptor 2 antibody,AT2R-Ab ELISA Kit 96T/48T
J00762 人血管紧张素Ⅱ受体1抗体(ATⅡR1)ELISA试剂盒 Human angiotension Ⅱ receptor 1 Antibody,ATⅡR1 Ab ELISA Kit 96T/48T
J00763 人血管紧张素Ⅰ受体抗体(ANG-ⅠR)ELISA试剂盒 Human angiotension I receptor Antibody,ANG-ⅠR antibody ELISA Kit 96T/48T
J00764 人抗钙调素特异抗体(CAM-ab)ELISA试剂盒 Human anti-calmodulin specific antibody,CaM-ab ELISA Kit 96T/48T
J00765 人甲状腺非肽激素抗体(THAA)ELISA试剂盒 Human thyroid hormone autoantibodies,THAA ELISA Kit 96T/48T
J00766 人抗类固醇生成细胞抗体(SCA)ELISA试剂盒 Human steroid producing cell autoantibody,SCA ELISA Kit 96T/48T
J00767 人抗信号识别颗粒抗体(SRP)ELISA试剂盒 Human signal recognization particle antibody,SRP ELISA Kit 96T/48T
J00768 人抗细胞膜DNA抗体(cmDNA)ELISA试剂盒 Human anti-cell membrane DNA antibody,cmDNA ELISA Kit 96T/48T
J00769 人抗钙蛋白酶抑素抗体(ACAST-DⅣ)ELISA试剂盒 Human autoantibodies against the C-terminal domain Ⅳ,ACAST-DⅣ ELISA Kit 96T/48T
J00770 人抗核仁纤维蛋白抗体(AFA/snoRNP/U3RNP)ELISA试剂盒 Human anti-fibrillarin antibody,AFA/snoRNP/U3RNP ELISA Kit 96T/48T
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J00772 人抗神经节苷脂抗体(GM1)ELISA试剂盒 Human anti-ganglioside antibody,GM1 ELISA Kit 96T/48T
J00773 人抗髓鞘相关糖蛋白抗体(MAG Ab)ELISA试剂盒 Human anti-myelin associated glycoprotein antibody,MAG Ab ELISA Kit 96T/48T
J00774 人抗中性粒细胞抗体(ANA)ELISA试剂盒 Human anti-neutrophil antibody,ANA ELISA Kit 96T/48T
J00775 人抗胃壁细胞抗体(AGPA/PCA)ELISA试剂盒 Human anti-gastric parietal cell antibody,AGPA/PCA ELISA Kit 96T/48T
J00776 人抗网硬蛋白抗体(ARA)ELISA试剂盒 Human anti-Reticulin antibody,ARA ELISA Kit 96T/48T
J00777 人抗突变型瓜氨酸波形蛋白抗体(MCV)ELISA试剂盒 Human anti-myelin antibody IgA,AMA IgA ELISA Kit 96T/48T
J00778 人抗髓磷脂抗体IgA(AMA IgA)ELISA试剂盒 Human anti-myelin antibody IgA,AMA IgA ELISA Kit 96T/48T
J00779 人抗腮腺管抗体(anti-parotid duct Ab)ELISA试剂盒 Human anti-parotid duct antibody ELISA Kit 96T/48T
J00780 人抗软骨抗体(anti-cartilage-Ab)ELISA试剂盒 Human anti-cartilage-antibody ELISA Kit 96T/48T
J00781 人抗人绒毛膜促性腺激素抗体(AhCGAb)ELISA试剂盒 Human anti-chorionic gonadotropin-antibody,AhCGAb ELISA Kit 96T/48T
J00782 人抗染色体抗体(anti-chromosome Ab)ELISA试剂盒 Human anti-chorionic gonadotropin-antibody,AhCGAb ELISA Kit 96T/48T
J00783 人抗脑组织抗体(ABAb)ELISA试剂盒 Human anti-brain tissue antibody,ABAb ELISA Kit 96T/48T
J00784 人抗麦胶蛋白/麦醇溶蛋白抗体(AGA)ELISA试剂盒 Human anti-gliadin antibody,AGA ELISA Kit 96T/48T
J00785 人抗磷脂酰丝氨酸抗体(APSA)ELISA试剂盒 Human Anti-phosphatidyl serine antibody,APSA ELISA Kit 96T/48T
J00786 人抗磷壁酸抗体(TA)ELISA试剂盒 Human anti-teichoic acid antibody,TA ELISA Kit 96T/48T
J00787 人抗淋巴细胞毒抗体(ALA/LCA)ELISA试剂盒 磷酸化细胞转录因子ELK1抗体Human anti-lymphocytotoxic antibody,ALA/LCA ELISA Kit 96T/48T
J00788 人抗巨噬细胞抗体(anti-macrophage Ab)ELISA试剂盒 Human anti-macrophage antibody ELISA Kit 96T/48T
J00789 人抗甲状腺过氧化物酶抗体(TPO-Ab)ELISA试剂盒 Human anti-Thyroid-Peroxidase antibody,TPO-Ab ELISA Kit 96T/48T
J00790 人抗红细胞抗体(RBC)ELISA试剂盒 Human anti-red cell antibody ELISA Kit 96T/48T
J00791 人28S抗核糖体抗体(28S rRNP)ELISA试剂盒 Human 28S ribosome RNP antibody,28S rRNP ELISA Kit 96T/48T
更多磷酸化细胞转录因子ELK1抗体价格、批发价、品牌、厂家及抗体详细说明书，都可以免费咨询及索取。
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