腺样癌特异性相关抗原EMC1抗体

在免疫化学技术中影响腺样癌特异性相关抗原EMC1抗体效率的因素主要有5个方面：①抗体对抗原的亲合性；②抗体对抗原的特异性；③抗原表位结构在该技术中是如何被改变的；④抗体与抗原结合的易接近性；⑤二级试剂的质量和类型
英文名称 Anti-EMC1
中文名称 腺样癌特异性相关抗原EMC1抗体
别 名 EMC1_HUMAN; ER membrane protein complex subunit 1; ER membrane protein complex subunit 1 precursor; 2700016F22Rik; C230096C10Rik; Emc1; Hypothetical protein LOC23065; K0090_HUMAN; KIAA0090; mKIAA0090; OTTHUMP00000215527; PSEC0263; RIKEN cDNA C230096C10 gene; RP1-43E13.1; RP23-371E13.1; Uncharacterized protein KIAA0090.
腺样癌特异性相关抗原EMC1抗体浓 度 1mg/1ml
规 格 0.1ml/100μg 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Dog, Rabbit
产品类型 一抗
研究领域 肿瘤 细胞生物 免疫学
蛋白分子量 predicted molecular weight: 107kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human EMC1
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 This gene encodes a single-pass type I transmembrane protein, which is a subunit of the endoplasmic reticulum membrane protein complex (EMC). Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2012].
Subunit : Component of the ER membrane protein complex (EMC).
Subcellular Location : Membrane; Single-pass type I membrane protein.
Similarity : Belongs to the EMC1 family.
Database links : UniProtKB/Swiss-Prot: Q8N766.1
半个多世纪以来，抗体一直作为常用的研究工具，因此对其使用和储存已有丰富的经验。这些经验让人们知道如何对抗体进行操作，由于抗体的稳定性好，使用并不困难。抗体较其他蛋白不易变性，易于存放、纯化和标记。蛋白G和蛋白A等抗体结合蛋白的发现使得这项工作更具可行性。
选择合适的抗体对免疫化学技术的成功极为重要。没有任何一种来源的抗体能够满足本书所述的各种方法的要求。为了研究某一重要的抗原，应选用具有不同特性的多种抗体。在没有一套良好特性的抗体可用于研究机体中每一种蛋白之前，选择及制备适合各种技术要求的抗体非常必要。