人类成纤维细胞系;CNLMG-B5537SKIN
形态特性 成纤维细胞样
生长特性 贴壁生长
特征特性 该细胞属专利保藏,其特征特性尚未公开。
培养条件 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle's Balanced Salts) 10%FBS
传代方法 1:3传代,3-4天传1次
传代情况 C6
冻存条件 基础培养基+5%DMSO+20%FBS
运输需要注意的问题:短途运输问题不大。尤其是长途天热时,我们要做好相应的运输安全措施。
人类成纤维细胞系;CNLMG-B5537SKIN复苏要快速溶解,一般从液氮罐中取出立即置于38度左右温水中快速摇动,溶解过程控制在一分钟之内最好。如果你放到保温瓶30分钟恐怕复苏的存活率很低。建议你买活的细胞!
活细胞的运输方法其实很简单,我曾经拿了一瓶细胞在路上耽搁了一个多小时回来细胞状态仍然很好。
具体做法是:
1)将该瓶细胞装满培养基,这样做的目的是让所有细胞都始终有营养供给。
2)用酒精擦拭培养瓶,瓶口用封口膜包好。
3)将培养瓶竖放到干净的塑料手套或纸带中。
4)细胞取回后,半量换液。
如果是由于意外断电,需要步行20分钟将细胞转移到另一个实验室,就没条件将培养瓶灌满培养基,建议你将瓶口用封口膜包好,瓶身外面用酒精擦拭后平放到铝饭盒(用来装物品消毒的,细胞实验室常见),水平端到目的地就没问题!不用过于担心,只要瓶口封好了一般不会污染;细胞置于培养箱外20分钟问题不大。
公司拥有完整的细胞培养实验室,并代理ATCC、IST-Banca、AllCells等品牌人类成纤维细胞系;CNLMG-B5537SKIN产品,各种属、各品牌细胞株,我们优势提供。
A09891 白介素25(IL25)酶联免疫试剂盒 ,英文名: ELISA Kit for Interleukin 25 (IL25) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09892 白介素26(IL26)酶联免疫试剂盒 ,英文名: ELISA Kit for Interleukin 26 (IL26) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09893 血管内皮生长因子165(VEGF165)酶联免疫试剂盒 ,英文名: ELISA Kit for Vascular Endothelial Growth Factor 165 (VEGF165) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09894 α1-抗胰蛋白酶(α1AT)酶联免疫试剂盒 ,英文名: ELISA Kit for Alpha-1-Antitrypsin (a1AT) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09895 乳酸脱氢酶B(LDHB)酶联免疫试剂盒 ,英文名: ELISA Kit for Lactate Dehydrogenase B (LDHB) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09896 乳酸脱氢酶B(LDHB)酶联免疫试剂盒 ,英文名: ELISA Kit for Lactate Dehydrogenase B (LDHB) ,适用种属: Rattus norvegicus (Rat) ,规格: 48T/96T
A09897 乳酸脱氢酶B(LDHB)酶联免疫试剂盒 ,英文名: ELISA Kit for Lactate Dehydrogenase B (LDHB) ,适用种属: Mus musculus (Mouse) ,规格: 48T/96T
A09898 血管生成素样蛋白3(ANGPTL3)酶联免疫试剂盒 ,英文名: ELISA Kit for Angiopoietin Like Protein 3 (ANGPTL3) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09899 白介素17B(IL17B)酶联免疫试剂盒 ,英文名: ELISA Kit for Interleukin 17B (IL17B) ,适用种属: Rattus norvegicus (Rat) ,规格: 48T/96T
A09900 WAP四二硫化物核心域蛋白1(WFDC1)酶联免疫试剂盒 ,英文名: ELISA Kit for WAP Four Disulfide Core Domain Protein 1 (WFDC1) ,适用种属: Mus musculus (Mouse) ,规格: 48T/96T
A09901 胰淀素(IAPP)酶联免疫试剂盒 ,英文名: ELISA Kit for Islet Amyloid Polypeptide (IAPP) ,适用种属: Oryctolagus cuniculus (Rabbit) ,规格: 48T/96T
A09902 核糖核酸酶A3(RNASE3)酶联免疫试剂盒 ,英文名: ELISA Kit for Ribonuclease A3 (RNASE3) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09903 TATA盒结合蛋白(TBP)酶联免疫试剂盒 ,英文名: ELISA Kit for TATA Binding Protein (TBP) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09904 肿瘤坏死因子α(TNFα)酶联免疫试剂盒 ,英文名: ELISA Kit for Tumor Necrosis Factor Alpha (TNFa) ,适用种属: Canis familiaris; Canine (Dog) ,规格: 48T/96T
A09905 粒细胞巨噬细胞集落刺激因子(GMCSF)酶联免疫试剂盒 ,英文名: ELISA Kit for Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) ,适用种属: Canis familiaris; Canine (Dog) ,规格: 48T/96T
A09906 髓分化因子88(MyD88)酶联免疫试剂盒 ,英文名: ELISA Kit for Myeloid Differentiation Factor 88 (MyD88) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09907 法尼基二磷酸法尼基转移酶1(FDFT1)酶联免疫试剂盒 ,英文名: ELISA Kit for Farnesyl Diphosphate Farnesyltransferase 1 (FDFT1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09908 T-细胞白血病/淋巴瘤蛋白1A(TCL1A)酶联免疫试剂盒 ,英文名: ELISA Kit for T-Cell Leukemia/Lymphoma Protein 1A (TCL1A) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09909 糖盏蛋白(GC)酶联免疫试剂盒 ,英文名: ELISA Kit for Glycocalicin (GC) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09910 Kruppel样因子5(KLF5)酶联免疫试剂盒 ,英文名: ELISA Kit for Kruppel Like Factor 5, Intestinal (KLF5) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09911 卷曲同源物8(FZD8)酶联免疫试剂盒 ,英文名: ELISA Kit for Frizzled Homolog 8 (FZD8) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09912 信号传导转录激活因子1(STAT1)酶联免疫试剂盒 ,英文名: ELISA Kit for Signal Transducer And Activator Of Transcription 1 (STAT1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09913 转化生长因子β3(TGFβ3)酶联免疫试剂盒 ,英文名: ELISA Kit for Transforming Growth Factor Beta 3 (TGFb3) ,适用种属: Sus scrofa; Porcine (Pig) ,规格: 48T/96T
A09914 蛋白激酶Bβ(PKBβ)酶联免疫试剂盒 ,英文名: ELISA Kit for Protein Kinase B Beta (PKBb) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09915 白介素2受体α(IL2Rα)酶联免疫试剂盒 ,英文名: ELISA Kit for Interleukin 2 Receptor Alpha (IL2Ra) ,适用种属: Sus scrofa; Porcine (Pig) ,规格: 48T/96T
A09916 丝裂原激活蛋白激酶激酶6(MAP2K6)酶联免疫试剂盒 ,英文名: ELISA Kit for Mitogen Activated Protein Kinase Kinase 6 (MAP2K6) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09917 血清/糖皮质激素调节激酶2(SGK2)酶联免疫试剂盒 ,英文名: ELISA Kit for Serum/Glucocorticoid Regulated Kinase 2 (SGK2) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09918 表面活性物质关联蛋白D(SPD)酶联免疫试剂盒 ,英文名: ELISA Kit for Surfactant Associated Protein D (SPD) ,适用种属: Rattus norvegicus (Rat) ,规格: 48T/96T
A09919 吡哆醛激酶(PDXK)酶联免疫试剂盒 ,英文名: ELISA Kit for Pyridoxal Kinase (PDXK) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09920 CD163分子(CD163)酶联免疫试剂盒 ,英文名: ELISA Kit for Cluster Of Differentiation (CD163) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09921 信号传导转录激活因子5B(STAT5B)酶联免疫试剂盒 ,英文名: ELISA Kit for Signal Transducer And Activator Of Transcription 5B (STAT5B) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09922 生长分化因子2(GDF2)酶联免疫试剂盒 ,英文名: ELISA Kit for Growth Differentiation Factor 2 (GDF2) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09923 癌蛋白诱导转录物3(OIT3)酶联免疫试剂盒 ,英文名: ELISA Kit for Oncoprotein Induced Transcript 3 (OIT3) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09924 B-细胞淋巴瘤因子3(Bcl3)酶联免疫试剂盒 ,英文名: ELISA Kit for B-Cell CLL/Lymphoma 3 (Bcl3) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09925 胱天蛋白酶4(CASP4)酶联免疫试剂盒 ,英文名: ELISA Kit for Caspase 4 (CASP4) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09926 激活STAT蛋白抑制因子1(PIAS1)酶联免疫试剂盒 ,英文名: ELISA Kit for Protein Inhibitor Of Activated STAT 1 (PIAS1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09927 信号传导转录激活因子3(STAT3)酶联免疫试剂盒 ,英文名: ELISA Kit for Signal Transducer And Activator Of Transcription 3 (STAT3) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09928 泛素D(UBD)酶联免疫试剂盒 ,英文名: ELISA Kit for Ubiquitin D (UBD) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09929 血小板型磷酸果糖激酶(PFKP)酶联免疫试剂盒 ,英文名: ELISA Kit for Phosphofructokinase, Platelet (PFKP) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09930 己糖激酶3(HK3)酶联免疫试剂盒 ,英文名: ELISA Kit for Hexokinase 3, White Cell (HK3) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09931 色氨酰tRNA合成酶(WARS)酶联免疫试剂盒 ,英文名: ELISA Kit for Tryptophanyl tRNA Synthetase (WARS) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09932 二氢嘧啶酶样蛋白3(DPYSL3)酶联免疫试剂盒 ,英文名: ELISA Kit for Dihydropyrimidinase Like Protein 3 (DPYSL3) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09933 肿瘤坏死因子配体超家族成员13(TNFSF13)酶联免疫试剂盒 ,英文名: ELISA Kit for Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09934 接触蛋白4(CNTN4)酶联免疫试剂盒 ,英文名: ELISA Kit for Contactin 4 (CNTN4) ,适用种属: Rattus norvegicus (Rat) ,规格: 48T/96T
A09935 Ⅰ类主要组织相容性复合体E(MHCE)酶联免疫试剂盒 ,英文名: ELISA Kit for Major Histocompatibility Complex Class I E (MHCE) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09936 谷氨酸受体相互作用蛋白1(GRIP1)酶联免疫试剂盒 ,英文名: ELISA Kit for Glutamate Receptor Interacting Protein 1 (GRIP1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09937 干扰素调节因子4(IRF4)酶联免疫试剂盒 ,英文名: ELISA Kit for Interferon Regulatory Factor 4 (IRF4) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09938 表皮转谷氨酰铵酶(TGM3)酶联免疫试剂盒 ,英文名: ELISA Kit for Transglutaminase 3, Epidermal (TGM3) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09939 肌动蛋白束蛋白(FSCN)酶联免疫试剂盒 ,英文名: ELISA Kit for Fascin (FSCN) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09940 核糖核酸酶A3(RNASE3)酶联免疫试剂盒 ,英文名: ELISA Kit for Ribonuclease A3 (RNASE3) ,适用种属: Mus musculus (Mouse) ,规格: 48T/96T
A09941 人类成纤维细胞系;CNLMG-B5537SKIN羟甲基戊二酰辅酶A合酶(HMGCS)酶联免疫试剂盒 ,英文名: ELISA Kit for Hydroxymethylglutaryl Coenzyme A Synthase (HMGCS) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09942 胰腺再生蛋白Iα(REG1α)酶联免疫试剂盒 ,英文名: ELISA Kit for Regenerating Islet Derived Protein 1 Alpha (REG1a) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T