小鼠成纤维细胞包装细胞;pA317 C1
形态特性 成纤维细胞样
生长特性 贴壁生长
特征特性 该细胞属专利保藏,其特征特性尚未公开。
培养条件 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle's Balanced Salts) 10%FBS
传代方法 1:3传代,3-4天传1次
传代情况 C7
冻存条件 基础培养基+5%DMSO+20%FBS
运输需要注意的问题:短途运输问题不大。尤其是长途天热时,我们要做好相应的运输安全措施。
小鼠成纤维细胞包装细胞;pA317 C1复苏要快速溶解,一般从液氮罐中取出立即置于38度左右温水中快速摇动,溶解过程控制在一分钟之内最好。如果你放到保温瓶30分钟恐怕复苏的存活率很低。建议你买活的细胞!
活细胞的运输方法其实很简单,我曾经拿了一瓶细胞在路上耽搁了一个多小时回来细胞状态仍然很好。
具体做法是:
1)将该瓶细胞装满培养基,这样做的目的是让所有细胞都始终有营养供给。
2)用酒精擦拭培养瓶,瓶口用封口膜包好。
3)将培养瓶竖放到干净的塑料手套或纸带中。
4)细胞取回后,半量换液。
如果是由于意外断电,需要步行20分钟将细胞转移到另一个实验室,就没条件将培养瓶灌满培养基,建议你将瓶口用封口膜包好,瓶身外面用酒精擦拭后平放到铝饭盒(用来装物品消毒的,细胞实验室常见),水平端到目的地就没问题!不用过于担心,只要瓶口封好了一般不会污染;细胞置于培养箱外20分钟问题不大。
公司拥有完整的细胞培养实验室,并代理ATCC、IST-Banca、AllCells等品牌小鼠成纤维细胞包装细胞;pA317 C1产品,各种属、各品牌细胞株,我们优势提供。
A09688 细胞附着蛋白相互作用蛋白(CYTIP)酶联免疫试剂盒 ,英文名: ELISA Kit for Cytohesin Interacting Protein (CYTIP) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09689 B-细胞连接蛋白(BLNK)酶联免疫试剂盒 ,英文名: ELISA Kit for B-Cell Linker Protein (BLNK) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09690 B-淋巴细胞趋化因子1(BLC1)酶联免疫试剂盒 ,英文名: ELISA Kit for B-Lymphocyte Chemoattractant 1 (BLC1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09691 生长调节致癌基因β(GROβ)酶联免疫试剂盒 ,英文名: ELISA Kit for Growth Regulated Oncogene Beta (GROb) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09692 生长调节致癌基因γ(GROγ)酶联免疫试剂盒 ,英文名: ELISA Kit for Growth Regulated Oncogene Gamma (GROg) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09693 磷脂酰肌醇特异性磷酯酶Cβ3(PLCβ3)酶联免疫试剂盒 ,英文名: ELISA Kit for Phospholipase C Beta 3, Phosphoinositide Specific (PLCb3) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09694 糖磷脂酰肌醇(GPI)酶联免疫试剂盒 ,英文名: ELISA Kit for Glycophosphatidylinositol (GPI) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09695 胸肾表达趋化因子(BRAK)酶联免疫试剂盒 ,英文名: ELISA Kit for Breast And Kidney Expressed Chemokine (BRAK) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09696 皮肤T-细胞虏获趋化因子(CTACK)酶联免疫试剂盒 ,英文名: ELISA Kit for Cutaneous T-Cell Attracting Chemokine (CTACK) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09697 D-二聚体(D2D)酶联免疫试剂盒 ,英文名: ELISA Kit for D-Dimer (D2D) ,适用种属: Oryctolagus cuniculus (Rabbit) ,规格: 48T/96T
A09698 抑制素βC(INHβC)酶联免疫试剂盒 ,英文名: ELISA Kit for Inhibin Beta C (INHbC) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09699 Endoglin蛋白(ENG)酶联免疫试剂盒 ,英文名: ELISA Kit for Endoglin (ENG) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09700 肝脂酶(LIPC)酶联免疫试剂盒 ,英文名: ELISA Kit for Lipase, Hepatic (LIPC) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09701 肝脂酶(LIPC)酶联免疫试剂盒 ,英文名: ELISA Kit for Lipase, Hepatic (LIPC) ,适用种属: Rattus norvegicus (Rat) ,规格: 48T/96T
A09702 肝脂酶(LIPC)酶联免疫试剂盒 ,英文名: ELISA Kit for Lipase, Hepatic (LIPC) ,适用种属: Mus musculus (Mouse) ,规格: 48T/96T
A09703 钙/钙调蛋白依赖性蛋白激酶Ⅱγ(CAMK2γ)酶联免疫试剂盒 ,英文名: ELISA Kit for Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g) ,适用种属: Rattus norvegicus (Rat) ,规格: 48T/96T
A09704 血管细胞粘附分子1(VCAM1)酶联免疫试剂盒 ,英文名: ELISA Kit for Vascular Cell Adhesion Molecule 1 (VCAM1) ,适用种属: Rhesus monkey (Simian) ,规格: 48T/96T
A09705 肿瘤坏死因子α(TNFα)酶联免疫试剂盒 ,英文名: ELISA Kit for Tumor Necrosis Factor Alpha (TNFa) ,适用种属: Rhesus monkey (Simian) ,规格: 48T/96T
A09706 干扰素α(IFNα)酶联免疫试剂盒 ,英文名: ELISA Kit for Interferon Alpha (IFNa) ,适用种属: Rhesus monkey (Simian) ,规格: 48T/96T
A09707 α-内啡肽(αEP)酶联免疫试剂盒 ,英文名: ELISA Kit for Alpha-Endorphin (aEP) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09708 单核细胞趋化蛋白1(MCP1)酶联免疫试剂盒 ,英文名: ELISA Kit for Monocyte Chemotactic Protein 1 (MCP1) ,适用种属: Rhesus monkey (Simian) ,规格: 48T/96T
A09709 巨噬细胞移动抑制因子(MIF)酶联免疫试剂盒 ,英文名: ELISA Kit for Macrophage Migration Inhibitory Factor (MIF) ,适用种属: Rhesus monkey (Simian) ,规格: 48T/96T
A09710 胰岛素(INS)酶联免疫试剂盒 ,英文名: ELISA Kit for Insulin (INS) ,适用种属: Rhesus monkey (Simian) ,规格: 48T/96T
A09711 金属硫蛋白1(MT1)酶联免疫试剂盒 ,英文名: ELISA Kit for Metallothionein 1 (MT1) ,适用种属: Bos taurus; Bovine (Cattle) ,规格: 48T/96T
A09712 干扰素γ(IFNγ)酶联免疫试剂盒 ,英文名: ELISA Kit for Interferon Gamma (IFNg) ,适用种属: Rhesus monkey (Simian) ,规格: 48T/96T
A09713 白介素6(IL6)酶联免疫试剂盒 ,英文名: ELISA Kit for Interleukin 6 (IL6) ,适用种属: Rhesus monkey (Simian) ,规格: 48T/96T
A09714 磷酸烯醇式丙酮酸羧激酶1(PCK1)酶联免疫试剂盒 ,英文名: ELISA Kit for Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) ,适用种属: Mus musculus (Mouse) ,规格: 48T/96T
A09715 肾上腺髓质素(ADM)酶联免疫试剂盒 ,英文名: ELISA Kit for Adrenomedullin (ADM) ,适用种属: Rattus norvegicus (Rat) ,规格: 48T/96T
A09716 肾上腺髓质素(ADM)酶联免疫试剂盒 ,英文名: ELISA Kit for Adrenomedullin (ADM) ,适用种属: Mus musculus (Mouse) ,规格: 48T/96T
A09717 白介素2受体β(IL2Rβ)酶联免疫试剂盒 ,英文名: ELISA Kit for Interleukin 2 Receptor Beta (IL2Rb) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09718 白介素2受体β(IL2Rβ)酶联免疫试剂盒 ,英文名: ELISA Kit for Interleukin 2 Receptor Beta (IL2Rb) ,适用种属: Rattus norvegicus (Rat) ,规格: 48T/96T
A09719 白介素2受体β(IL2Rβ)酶联免疫试剂盒 ,英文名: ELISA Kit for Interleukin 2 Receptor Beta (IL2Rb) ,适用种属: Mus musculus (Mouse) ,规格: 48T/96T
A09720 转录因子20(TCF20)酶联免疫试剂盒 ,英文名: ELISA Kit for Transcription factor 20 (TCF20) ,适用种属: Mus musculus (Mouse) ,规格: 48T/96T
A09721 热休克转录因子1(HSF1)酶联免疫试剂盒 ,英文名: ELISA Kit for Heat Shock Transcription Factor 1 (HSF1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09722 热休克转录因子1(HSF1)酶联免疫试剂盒 ,英文名: ELISA Kit for Heat Shock Transcription Factor 1 (HSF1) ,适用种属: Mus musculus (Mouse) ,规格: 48T/96T
A09723 白介素18(IL18)酶联免疫试剂盒 ,英文名: ELISA Kit for Interleukin 18 (IL18) ,适用种属: Chicken (Gallus) ,规格: 48T/96T
A09724 干扰素α(IFNα)酶联免疫试剂盒 ,英文名: ELISA Kit for Interferon Alpha (IFNa) ,适用种属: Chicken (Gallus) ,规格: 48T/96T
A09725 抗载脂蛋白抗体(AAHA)酶联免疫试剂盒 ,英文名: ELISA Kit for Anti-Apolipoprotein Antibodies (AAHA) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09726 淋巴细胞胞浆蛋白2(LCP2)酶联免疫试剂盒 ,英文名: ELISA Kit for Lymphocyte Cytosolic Protein 2 (LCP2) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09727 T-细胞激活连接蛋白(LAT)酶联免疫试剂盒 ,英文名: ELISA Kit for Linker For Activation Of T-Cell (LAT) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09728 蛋白酪氨酸磷酸酶受体H(PTPRH)酶联免疫试剂盒 ,英文名: ELISA Kit for Protein Tyrosine Phosphatase Receptor Type H (PTPRH) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09729 神经氨酸酶(NEU)酶联免疫试剂盒 ,英文名: ELISA Kit for Neuraminidase (NEU) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09730 原钙黏素α1(PCDHα1)酶联免疫试剂盒 ,英文名: ELISA Kit for Protocadherin Alpha 1 (PCDHa1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09731 磷酯酶Cγ1(PLCγ1)酶联免疫试剂盒 ,英文名: ELISA Kit for Phospholipase C Gamma 1 (PLCg1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09732 Ras GTP酶激活蛋白1(RASA1)酶联免疫试剂盒 ,英文名: ELISA Kit for Ras GTPase Activating Protein 1 (RASA1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09733 GDP解离抑制因子1(GDI1)酶联免疫试剂盒 ,英文名: ELISA Kit for GDP Dissociation Inhibitor 1 (GDI1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09734 二铵氧化酶(DAO)酶联免疫试剂盒 ,英文名: ELISA Kit for Diamine Oxidase (DAO) ,适用种属: Mus musculus (Mouse) ,规格: 48T/96T
A09735 细胞间粘附分子5(ICAM5)酶联免疫试剂盒 ,英文名: ELISA Kit for Intercellular Adhesion Molecule 5 (ICAM5) ,适用种属: Oryctolagus cuniculus (Rabbit) ,规格: 48T/96T
A09736 血栓素B2(TXB2)酶联免疫试剂盒 ,英文名: ELISA Kit for Thromboxane B2 (TXB2) ,适用种属: Oryctolagus cuniculus (Rabbit) ,规格: 48T/96T
A09737 激肽释放酶10(KLK10)酶联免疫试剂盒 ,英文名: ELISA Kit for Kallikrein 10 (KLK10) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09738 激肽释放酶11(KLK11)酶联免疫试剂盒 ,英文名: ELISA Kit for Kallikrein 11 (KLK11) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09739 9kDa信号识别颗粒(SRP9)酶联免疫试剂盒 ,英文名: ELISA Kit for Signal Recognition Particle 9kDa (SRP9) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09740 粒细胞特异性抗核抗体(GSANA)酶联免疫试剂盒 ,英文名: ELISA Kit for Granulocyte Specific Anti-Nuclear Antibody (GSANA) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09741 甲状腺激素自身抗体(THAA)酶联免疫试剂盒 ,英文名: ELISA Kit for Thyroid Hormone Autoantibody (THAA) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09742 周期素依赖性激酶5(CDK5)酶联免疫试剂盒 ,英文名: ELISA Kit for Cyclin Dependent Kinase 5 (CDK5) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09743 小鼠成纤维细胞包装细胞;pA317 C1环加氧酶2(PTGS2)酶联免疫试剂盒 ,英文名: ELISA Kit for Prostaglandin Endoperoxide Synthase 2 (PTGS2) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09744 Dickkopf相关蛋白1(DKK1)酶联免疫试剂盒 ,英文名: ELISA Kit for Dickkopf Related Protein 1 (DKK1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A09745 羟赖氨酸(Hyl)酶联免疫试剂盒 ,英文名: ELISA Kit for Hydroxylysine (Hyl) ,适用种属: General ,规格: 48T/96T
A09746 金属硫蛋白3(MT3)酶联免疫试剂盒 ,英文名: ELISA Kit for Metallothionein 3 (MT3) ,适用种属: Bos taurus; Bovine (Cattle) ,规格: 48T/96T