Streptavidin
Streptavidin
Catalog No. PRO-283
Introduction:
Streptavidin is a tetrameric protein secreted by Streptomyces avidinii which binds firmly to biotin. Streptavidin is wilyde used in molecular biology through its unique high affinity for the vitamin biotin. The dissociation constant (Kd) of the biotin-streptavidin complex is about ~10-15 mol/L. The strong affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. The streptavidin/biotin system has one of the biggest free energies of association of yet observed for noncovalent binding of a protein and small ligand in aqueous solution (K_assoc = 10**14). The complexes are also extremely stable over a wide range of temperature and pH.
Description:
The Streptavidin preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native streptavidin. Streptavidin has a molecular weight of 53 kDa.
Source:
Bacterium Streptomyces avidinii
Physical Appearance:
Sterile Filtered lyophilized powder.
Formulation:
Lyophilized in 10mM potassium phosphate buffer pH 6.5.
Solubility:
Gives a clear solution at 5mg/ml in 0.1M NaCl.
Stability:
Streptavidin although stable at 4°C for 3 weeks, should be stored desiccated below -18°C.
For longer storage in dissolved form add 1mM EDTA and/or 0.02 % NaN3 or pass the solution through a sterile filter.
Please prevent freeze-thaw cycles.
Application:
Streptavidin may be used to visualize biotin conjugated molecules in ELISA, blotting and histological technique.
Specific Activity:
Greater than 15.3 U/mg, 1 unit binds 1µg biotin.
Proteolytic Activity:
Less than 10-3 U/mg protein (Azocoll, 25°C, 24 h, pH 8.0).
Purity:
Greater than 95.0% as determined by SDS-PAGE.
Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Related Products
» Streptavidin, His
» Streptavidin-NC
更多
企业名称
上海高创化学科技有限公司
企业信息已认证
企业类型
信用代码
310228001353263
成立日期
2010-06-05
注册资本
10
经营范围
从事化学科技、计算机领域内技术开发、技术咨询、技术服务,从事生物科技领域内技术开发、技术咨询,化学试剂、化工原料及产品(除危险化学品、监控化学品、烟花爆竹、民用爆炸物品、易制毒化学品),实验室设备(除医疗器械),仪器仪表,Ⅰ类医疗器械,金属制品,电子产品,机电产品销售。【依法须经批准的项目,经相关部门批准后方可开展经营活动】
上海高创化学科技有限公司
公司地址
上海市涞亭南路888弄238号
客服电话